Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EW998_RS20985 Genome accession   NZ_CP035874
Coordinates   4322221..4322661 (+) Length   146 a.a.
NCBI ID   WP_000360896.1    Uniprot ID   -
Organism   Escherichia coli strain B2C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4317221..4327661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW998_RS20965 aroP 4318219..4319592 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EW998_RS20970 ampE 4319635..4320489 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EW998_RS20975 ampD 4320486..4321037 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EW998_RS20980 nadC 4321125..4322018 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EW998_RS20985 pilA 4322221..4322661 (+) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  EW998_RS20990 pilB 4322671..4324056 (+) 1386 WP_001025156.1 type II secretion system protein GspE Machinery gene
  EW998_RS20995 hofC 4324046..4325248 (+) 1203 WP_000157223.1 protein transport protein HofC -
  EW998_RS21000 guaC 4325283..4326326 (-) 1044 WP_001217351.1 GMP reductase -
  EW998_RS21005 - 4326482..4326526 (-) 45 WP_120795372.1 protein YacM -
  EW998_RS21010 coaE 4326551..4327171 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15647.84 Da        Isoelectric Point: 4.3938

>NTDB_id=306262 EW998_RS20985 WP_000360896.1 4322221..4322661(+) (pilA) [Escherichia coli strain B2C]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQEILNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=306262 EW998_RS20985 WP_000360896.1 4322221..4322661(+) (pilA) [Escherichia coli strain B2C]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAATTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363