Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   DV131_RS01955 Genome accession   NZ_CP031246
Coordinates   340672..341133 (-) Length   153 a.a.
NCBI ID   WP_000250520.1    Uniprot ID   C1CA95
Organism   Streptococcus pneumoniae strain M26368     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 335672..346133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV131_RS01925 - 335976..336800 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  DV131_RS01930 rnpA 336775..337146 (-) 372 WP_000739246.1 ribonuclease P protein component -
  DV131_RS12050 - 337163..337294 (-) 132 WP_000768904.1 hypothetical protein -
  DV131_RS01935 - 337295..338485 (-) 1191 WP_000167766.1 acetate kinase -
  DV131_RS01940 comYH 338536..339489 (-) 954 WP_000345133.1 class I SAM-dependent methyltransferase Machinery gene
  DV131_RS01945 - 339550..340144 (-) 595 Protein_342 methyltransferase domain-containing protein -
  DV131_RS01950 comGG/cglG 340281..340694 (-) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  DV131_RS01955 comGF/cglF 340672..341133 (-) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  DV131_RS01960 comGE/cglE 341096..341398 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  DV131_RS01965 comGD/cglD 341361..341795 (-) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  DV131_RS01970 comGC/cglC 341758..342081 (-) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  DV131_RS01975 comGB/cglB 342083..343099 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  DV131_RS01980 comGA/cglA/cilD 343047..343988 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  DV131_RS01985 - 344063..344428 (-) 366 WP_000286415.1 DUF1033 family protein -
  DV131_RS01990 - 344579..345637 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17905.50 Da        Isoelectric Point: 8.0031

>NTDB_id=304684 DV131_RS01955 WP_000250520.1 340672..341133(-) (comGF/cglF) [Streptococcus pneumoniae strain M26368]
MVQNSCWQSKSHKVKAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=304684 DV131_RS01955 WP_000250520.1 340672..341133(-) (comGF/cglF) [Streptococcus pneumoniae strain M26368]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGATTGCCCTGAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1CA95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909

Multiple sequence alignment