Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   soil367_RS03465 Genome accession   NZ_CP031093
Coordinates   758093..760264 (+) Length   723 a.a.
NCBI ID   WP_136546932.1    Uniprot ID   A0A4P7XEM7
Organism   Hydrocarboniclastica marina strain soil36-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 753093..765264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  soil367_RS03445 (soil367_03455) pilM 755283..756353 (+) 1071 WP_136546924.1 pilus assembly protein PilM Machinery gene
  soil367_RS03450 (soil367_03460) - 756353..756919 (+) 567 WP_136546926.1 PilN domain-containing protein -
  soil367_RS03455 (soil367_03465) pilO 756916..757542 (+) 627 WP_136546928.1 type 4a pilus biogenesis protein PilO -
  soil367_RS03460 (soil367_03470) - 757539..758096 (+) 558 WP_136546930.1 pilus assembly protein PilP -
  soil367_RS03465 (soil367_03475) pilQ 758093..760264 (+) 2172 WP_136546932.1 type IV pilus secretin PilQ family protein Machinery gene
  soil367_RS03470 (soil367_03480) - 760987..761796 (+) 810 WP_136546935.1 THxN family PEP-CTERM protein -
  soil367_RS03475 (soil367_03485) aroK 762023..762565 (+) 543 WP_136546937.1 shikimate kinase AroK -
  soil367_RS03480 (soil367_03490) aroB 762579..763676 (+) 1098 WP_136546939.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 723 a.a.        Molecular weight: 78003.10 Da        Isoelectric Point: 4.2216

>NTDB_id=303291 soil367_RS03465 WP_136546932.1 758093..760264(+) (pilQ) [Hydrocarboniclastica marina strain soil36-7]
MIDRNINAQKRFGLGLEMFSKLKSLIAVAVLGAGANLAWGVTLEDLDFASLPGDRIEVRLSFDGTPPAPSGYTIEQPARI
ALDLEGVDSGLSDRYHNLGVGNTQSMTVIDAKGRTRLIFNLSQLAPYDTRVEGNALIVEIGGGAGNDDSPSADEPLQAST
SPQQPEAQAQGQGESSAAASITGRSVNEVDFRRGADGDGQVVITLSDPRTSVDLSETAGNIRLRLADTQLPESERRRLNV
NDFATPVRQIETYTEGDAVIIDIDAEGDYDYLAYQSGNQFTVAVEELSQAESEERREEKFPYSGEKLSLNFQDIEVRSVL
QLIADFTGLNLVASDTVGGSLTLRLDSVPWDQALELILKTKGLDKRQVGNVLLVAPASEIADRERLELENNRQQRELAPV
RLDIVQVNYAKASDLVALLQQDAELISGRGFISSDARTNTISVRETAEKLEQIRRLVSTWDIPVRQVLIEARIVRAQTNI
ARDLGVQWGGGFAKQNGNSRISAGGSQTTLSEIDNAFTSGSPAQISFPGALAVDLGVSRASTSSFAIGYSNNDFLIDLEL
SALESDGKAEIVAQPKVVTADRQTAIIESGEEIPYQEATSSGAASISFKEAVLGLEVTPQITPDDNIIMDLVVRQDSRGE
VTAGIPSINTNEVETQVLVANGETVVLGGIFQSEIATTITKTPFLGDIPYLGWLFTRSEKIDERSELLIFITPKIVKSSL
TSN

Nucleotide


Download         Length: 2172 bp        

>NTDB_id=303291 soil367_RS03465 WP_136546932.1 758093..760264(+) (pilQ) [Hydrocarboniclastica marina strain soil36-7]
ATGATTGACCGCAACATTAACGCACAAAAACGCTTTGGATTGGGGCTAGAGATGTTCAGCAAATTGAAGAGCTTGATTGC
TGTTGCGGTATTGGGCGCGGGCGCGAACCTGGCCTGGGGTGTGACCCTGGAGGATCTGGATTTCGCATCGCTGCCCGGCG
ACCGCATTGAGGTCAGGCTGTCGTTTGACGGTACACCTCCGGCTCCGTCGGGCTACACCATCGAACAGCCCGCTCGTATC
GCGCTGGACCTGGAAGGTGTGGATAGTGGCCTGAGTGACCGGTACCACAACCTCGGTGTGGGTAACACGCAGAGCATGAC
GGTTATTGATGCAAAGGGGCGCACCCGGCTGATTTTCAACCTGTCTCAGCTTGCGCCGTATGACACGCGAGTAGAGGGCA
ACGCCCTGATCGTGGAAATCGGCGGCGGCGCGGGTAATGATGATAGCCCGAGCGCCGACGAGCCTCTGCAGGCCTCGACC
TCGCCGCAACAGCCTGAAGCCCAGGCACAGGGGCAGGGCGAGAGCTCCGCCGCCGCGTCGATCACCGGTCGCAGTGTCAA
CGAAGTTGATTTCCGCCGCGGCGCTGATGGTGATGGGCAGGTGGTCATTACGTTGTCTGACCCGAGAACTTCAGTTGATC
TCAGTGAAACAGCCGGCAACATTCGTCTGCGGCTCGCTGATACCCAGTTGCCGGAATCCGAGCGCCGTCGACTCAACGTC
AATGACTTCGCAACTCCGGTACGACAGATCGAAACCTATACCGAAGGCGATGCGGTCATCATTGATATCGACGCCGAAGG
CGATTACGACTACCTCGCCTACCAGTCGGGCAACCAGTTCACGGTAGCAGTAGAAGAGCTGAGCCAGGCCGAGAGCGAAG
AGCGCCGCGAGGAGAAGTTCCCGTACAGCGGAGAAAAGCTGTCACTCAATTTTCAGGATATCGAAGTGCGCTCGGTGCTC
CAGCTGATCGCTGATTTCACCGGTCTCAATCTGGTCGCATCCGACACGGTCGGCGGTAGCCTGACGTTGCGCCTGGACAG
CGTTCCATGGGACCAGGCCCTTGAGTTGATACTCAAGACCAAAGGCCTGGACAAACGCCAGGTCGGGAACGTTCTTCTAG
TCGCACCGGCGAGTGAGATCGCCGACCGCGAACGCCTGGAGCTGGAAAATAACCGCCAGCAGCGAGAGCTTGCGCCGGTT
CGCCTCGACATCGTACAGGTGAATTACGCCAAAGCGTCGGATCTTGTGGCTTTGCTGCAGCAGGACGCTGAACTGATTTC
CGGGCGGGGCTTTATTTCCTCCGACGCACGCACCAACACCATAAGTGTGCGGGAGACTGCTGAAAAGCTTGAGCAGATTC
GGCGACTTGTCAGTACCTGGGACATCCCGGTGCGCCAGGTTCTGATCGAGGCTCGTATCGTCCGTGCGCAGACCAACATT
GCCCGGGATCTGGGTGTGCAGTGGGGCGGCGGCTTCGCCAAGCAGAATGGCAACAGCCGTATCTCTGCGGGCGGTTCGCA
AACGACTCTGTCTGAAATCGACAATGCGTTTACCAGCGGCAGCCCTGCGCAAATCTCATTCCCGGGCGCATTGGCGGTAG
ACCTGGGTGTCAGCCGAGCCAGTACCTCAAGTTTTGCTATCGGCTACAGCAACAACGATTTTCTCATCGATCTGGAGTTG
TCTGCACTGGAAAGCGACGGTAAAGCCGAAATTGTGGCTCAACCAAAAGTGGTCACGGCCGACCGTCAAACCGCGATCAT
TGAGTCTGGTGAAGAGATTCCCTATCAGGAAGCGACCTCCAGCGGCGCAGCATCAATCTCCTTTAAAGAGGCTGTACTGG
GCCTGGAAGTAACGCCACAAATTACGCCGGATGACAACATCATCATGGACCTGGTGGTACGGCAGGACTCCCGTGGCGAA
GTTACCGCGGGGATCCCGAGTATCAACACCAACGAGGTTGAAACGCAGGTTCTGGTAGCCAACGGTGAAACGGTAGTGCT
CGGTGGAATCTTCCAGTCCGAGATAGCGACGACCATTACCAAGACGCCGTTCCTTGGTGATATCCCTTACCTCGGCTGGC
TATTCACGCGCTCCGAGAAAATCGATGAGCGGAGCGAGCTGCTTATCTTCATAACACCCAAGATCGTGAAAAGCAGCCTG
ACGAGCAATTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(330-376)

AMIN domain (AMIN)

(54-140)

Bacterial type II/III secretion system short domain (Secretin_N)

(404-466)

Bacterial type II and III secretion system protein (Secretin)

(562-717)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7XEM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.311

100

0.495
  pilQ Acinetobacter baumannii D1279779

39.648

100

0.405
  pilQ Acinetobacter baumannii strain A118

39.648

100

0.405
  comQ Acinetobacter baylyi ADP1

41.026

97.095

0.398
  pilQ Legionella pneumophila strain Lp02

37.431

100

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.431

100

0.375

Multiple sequence alignment