Detailed information NTG0303290

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: soil367_RS03445
• Genome accession: NZ_CP031093
• Coordinates: 755283..756353 (+)
• Length: 356 a.a.
• NCBI ID: WP_136546924.1
• Uniprot ID: A0A4P7XDX7
• Organism: Hydrocarboniclastica marina strain soil36-7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 750283..761353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
soil367_RS03430 (soil367_03440)	rpmE	750469..750690 (+)	222	WP_136546918.1	50S ribosomal protein L31	-
soil367_RS03435 (soil367_03445)	-	750995..752260 (+)	1266	WP_136546920.1	malic enzyme-like NAD(P)-binding protein	-
soil367_RS03440 (soil367_03450)	-	752404..754971 (-)	2568	WP_136546922.1	penicillin-binding protein 1A	-
soil367_RS03445 (soil367_03455)	pilM	755283..756353 (+)	1071	WP_136546924.1	pilus assembly protein PilM	Machinery gene
soil367_RS03450 (soil367_03460)	-	756353..756919 (+)	567	WP_136546926.1	PilN domain-containing protein	-
soil367_RS03455 (soil367_03465)	pilO	756916..757542 (+)	627	WP_136546928.1	type 4a pilus biogenesis protein PilO	-
soil367_RS03460 (soil367_03470)	-	757539..758096 (+)	558	WP_136546930.1	pilus assembly protein PilP	-
soil367_RS03465 (soil367_03475)	pilQ	758093..760264 (+)	2172	WP_136546932.1	type IV pilus secretin PilQ family protein	Machinery gene

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 38003.01 Da
• Isoelectric Point: 4.1668

>NTDB_id=303290 soil367_RS03445 WP_136546924.1 755283..756353(+) (pilM) [Hydrocarboniclastica marina strain soil36-7]
MFGFLTKKGSPVLGIDISSSSIKLLELSKQGDRYRVESYAVEPLPANSVVEKNIADVEAVGEAIKKVVGKAHASAKRAAV
AVSGSAVITKVIQMNAGMNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSETNPDQVDVLLAACRKENVDIREDALQV
AGLSARVVDVEAYAMERAFSMIESQLETTGEEQVVAIVDVGATMTTLSVLVDGETVYTREQLFGGRQLTEEIQRRYGLST
EEAGLAKKQGGGGLPDDYEGEVLAPFREAVVQQVARALQFFFGSSQYNTVDYVILAGGTASVTGLSEMVEAKVGTATMIA
NPFADMAVGSKVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1071 bp

>NTDB_id=303290 soil367_RS03445 WP_136546924.1 755283..756353(+) (pilM) [Hydrocarboniclastica marina strain soil36-7]
GTGTTCGGATTTCTAACGAAAAAAGGCAGTCCGGTTTTGGGCATCGACATCAGTTCGAGCTCGATCAAACTGCTTGAGTT
ATCAAAACAGGGCGACCGCTACCGCGTCGAAAGCTACGCGGTTGAGCCACTGCCCGCCAATTCCGTGGTGGAAAAGAACA
TTGCTGATGTGGAAGCTGTGGGTGAAGCCATAAAGAAAGTGGTTGGAAAAGCCCATGCGAGTGCTAAACGGGCCGCCGTC
GCGGTCTCGGGGTCAGCGGTCATCACCAAGGTCATCCAGATGAACGCGGGTATGAACGAGTTCGAAATGGAGGATCAGAT
TACCCTCGAAGCCGACCAGTACATTCCTTATCCGCTCGATGAAGTGGCGATAGATTTTGAGGTTCAGGGCCCGTCGGAAA
CCAATCCGGATCAGGTGGATGTCCTGTTGGCCGCCTGCCGCAAAGAGAACGTCGATATTCGTGAGGACGCGTTGCAGGTT
GCAGGGCTCTCGGCACGGGTGGTTGATGTGGAGGCTTATGCGATGGAACGGGCCTTCAGCATGATCGAGTCCCAGTTGGA
AACAACCGGCGAAGAGCAGGTTGTGGCCATCGTTGACGTGGGCGCGACTATGACCACGCTCAGCGTGCTGGTGGACGGCG
AGACCGTCTATACCCGGGAGCAGCTGTTCGGAGGCCGCCAGCTGACGGAGGAGATCCAGCGCCGGTACGGCTTATCTACA
GAAGAAGCTGGCCTGGCCAAAAAGCAGGGTGGAGGAGGCCTTCCCGACGACTACGAGGGTGAAGTTCTCGCGCCCTTCCG
GGAGGCCGTCGTCCAACAGGTTGCGCGCGCGTTGCAGTTCTTTTTCGGTTCGAGCCAATACAACACGGTCGACTACGTCA
TTCTCGCCGGCGGGACCGCATCGGTCACGGGCCTGAGCGAGATGGTCGAGGCCAAAGTGGGTACGGCGACCATGATTGCC
AATCCCTTTGCCGACATGGCGGTCGGCTCCAAAGTTAATGCGTCGGCCCTGAGTAATGATGCACCTTCCCTGATGATCGC
CTGCGGCTTGGCGATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-355)

3D structure

• AlphaFold DB: A0A4P7XDX7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	52.809	100	0.528
comM	Acinetobacter nosocomialis M2	52.809	100	0.528
comM	Acinetobacter baylyi ADP1	53.429	98.315	0.525
pilM	Legionella pneumophila strain ERS1305867	44.944	100	0.449