Detailed information NTG0302835

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DT536_RS06495
• Genome accession: NZ_CP031011
• Coordinates: 1352268..1353326 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain LXL_C1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1347268..1358326

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DT536_RS06480 (DT536_06480)	mraY	1347547..1348665 (+)	1119	WP_058870423.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
DT536_RS06485 (DT536_06485)	-	1348736..1349542 (+)	807	WP_058870422.1	putative RNA methyltransferase	-
DT536_RS06490 (DT536_06490)	ponA	1349574..1352102 (-)	2529	WP_114392656.1	penicillin-binding protein PBP1a	-
DT536_RS06495 (DT536_06495)	pilM	1352268..1353326 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
DT536_RS06500 (DT536_06500)	comN	1353326..1353982 (+)	657	WP_058870419.1	PilN domain-containing protein	Machinery gene
DT536_RS06505 (DT536_06505)	pilO	1353979..1354701 (+)	723	WP_058870418.1	type 4a pilus biogenesis protein PilO	Machinery gene
DT536_RS06510 (DT536_06510)	pilP	1354701..1355228 (+)	528	WP_058870417.1	pilus assembly protein PilP	Machinery gene
DT536_RS06515 (DT536_06515)	pilQ	1355248..1357311 (+)	2064	WP_058870416.1	type IV pilus secretin PilQ family protein	Machinery gene
DT536_RS06520 (DT536_06520)	aroK	1357349..1357882 (+)	534	WP_004978075.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=302835 DT536_RS06495 WP_058870420.1 1352268..1353326(+) (pilM) [Acinetobacter johnsonii strain LXL_C1]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=302835 DT536_RS06495 WP_058870420.1 1352268..1353326(+) (pilM) [Acinetobacter johnsonii strain LXL_C1]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAAGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCGCTG
GCAGTTCCGACATCTATGGTGATTACTAAAATTATCGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCAAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTTCCGAAGCATTGGAATTA
GGTGGTTTAACACCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATCGGTCATACCATGACCACCTTATCTGTCATGCAAAACAATAAAGTCA
TTTATACGCGTGAACAGGTGTTCGGTGGTAAACAACTGACTCAAGAAATTCAAAACCGCTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCTTGCCTGACGACTATGATATTGAAGTACTTGAACCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTCTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACCATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412