Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DPR03_RS01165 Genome accession   NZ_CP030754
Coordinates   244501..246210 (-) Length   569 a.a.
NCBI ID   WP_071319780.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain H3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 239501..251210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPR03_RS01135 - 239606..239929 (+) 324 WP_004810087.1 pyrimidine/purine nucleoside phosphorylase -
  DPR03_RS01140 rlmB 240082..240831 (+) 750 WP_004810085.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DPR03_RS01145 - 240838..241761 (+) 924 WP_081405839.1 DMT family transporter -
  DPR03_RS01150 coaE 241782..242378 (-) 597 WP_071319778.1 dephospho-CoA kinase -
  DPR03_RS01155 pilD 242380..243240 (-) 861 WP_071319779.1 prepilin peptidase Machinery gene
  DPR03_RS01160 pilC 243240..244469 (-) 1230 WP_004810078.1 type II secretion system F family protein Machinery gene
  DPR03_RS01165 pilB 244501..246210 (-) 1710 WP_071319780.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DPR03_RS01170 tpiA 246493..247284 (+) 792 WP_071319781.1 triose-phosphate isomerase -
  DPR03_RS01175 secG 247298..247627 (+) 330 WP_004810073.1 preprotein translocase subunit SecG -
  DPR03_RS01205 rimP 248674..249198 (+) 525 WP_004810072.1 ribosome maturation factor RimP -
  DPR03_RS01210 nusA 249234..250718 (+) 1485 WP_004810069.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63523.71 Da        Isoelectric Point: 4.8758

>NTDB_id=301311 DPR03_RS01165 WP_071319780.1 244501..246210(-) (pilB) [Acinetobacter schindleri strain H3]
MTAIQGSPRFTGFIRRLVDEGVISAEDMQGALSSAKKDKIDIVPYLIEHHRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNSNILFIATSNPTNIEAIDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDIDNSDPNKDDEDDNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKPADIPRQSLLEMGFS
DDDLQNPEFSIFEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=301311 DPR03_RS01165 WP_071319780.1 244501..246210(-) (pilB) [Acinetobacter schindleri strain H3]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGGGTTATATCTGCAGAAGA
TATGCAGGGTGCTTTGAGCAGTGCCAAAAAAGATAAAATTGATATTGTTCCTTATCTGATTGAACATCACCGCCTCTCGC
CAACCAAAATCGCAGAAACGATTTCTCTTGAATTCGGTGAACCGCTATTTGACATCAGTGCTTATGATCCTGCCCTGATC
GTACGCGAAGATTTTGATGAAAAGATTATTACCAAACACCGCGTGCTACCAATCTTTAAAAATAGCAATATTCTGTTTAT
CGCGACCAGCAACCCGACCAATATCGAAGCAATTGATGCTGTCCGTTTTGCGACCAAACTCAATATTGAAACCATCATTG
TTGAACATAATAAACTCGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGACTTTGATGATGATTTTGAT
CTAGATGTTGATATCGATAACTCTGACCCGAATAAAGATGATGAGGATGATAATAAAGGTGATGAAGCACCCATTGTTAA
ATATATTAATAAGTTACTGATTGATGCAATCCGTATGGGTGCATCTGATTTACACTTTGAACCTTATGAAAAAATGTACC
GTGTGCGTTACCGTGTCGACGGGGTCTTGCGTCAAATCGCCACGCCACCTTTACAGCTTGCAACACGTCTGGCTTCACGT
CTTAAAGTTATGTCACAAATGGATATTTCCGAGAAGCGTGTGCCTCAAGATGGCCGGATCAAGTTAAAACTTTCTAAAAA
TAAGGCCATTGACTTCCGTGTCAACTCACTCCCAACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATCCATCGA
GTGCCATGCTGGGTATTGATGCTTTGGGGTATGAGCCTGATCAAAAAGCCTTATTTATGGAGGCACTAGATAAGCCTCAA
GGTATGCTGTTAATTACCGGTCCGACAGGCTCTGGTAAAACTGTATCTCTTTATACCGGTTTAAATATCCTGAATCGTGA
AGACACCAATATTTCAACTGCTGAAGATCCCGTTGAAATTAACTTGCAAGGGATCAACCAGGTTAACGTCAATAATAAAG
TCGGCCTAACCTTCTCTGCTGCCTTGAAATCATTTCTGCGCCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAC
CTGGAAACTGCAGAGATTGCAATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACACTACATACCAACAGCGCACC
AGAAACCCTGACCCGTTTACGTAATATGGGTGTGCCATCATTTAATATTGCCACCTCGGTGAATTTGGTGATTGCTCAGC
GTTTGGCGCGTCGCCTTTGTCCACATTGCAAAAAACCGGCAGATATTCCACGTCAGAGCCTGCTTGAAATGGGCTTTAGC
GATGATGATTTACAGAATCCGGAATTTAGTATTTTTGAACCTGTCGGATGTAACGAATGTCGTGAAGGGTATAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCAGAGATTTCACGTATTATTATGGAAGATGGCAATGCAATTGAGATTG
CCGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.649

100

0.798
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.037

94.903

0.494
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio cholerae strain A1552

48.501

99.649

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.192

97.891

0.482
  pilB Vibrio campbellii strain DS40M4

48.115

97.891

0.471
  pilF Thermus thermophilus HB27

37.173

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

34.804

100

0.374

Multiple sequence alignment