Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XapA_RS18330 Genome accession   NZ_CP030164
Coordinates   4071820..4073556 (-) Length   578 a.a.
NCBI ID   WP_174854896.1    Uniprot ID   -
Organism   Xanthomonas citri pv. punicae strain LMG7439     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066820..4078556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XapA_RS18305 (XapA_18235) - 4067567..4069180 (+) 1614 WP_005930973.1 HAMP domain-containing sensor histidine kinase -
  XapA_RS18310 (XapA_18240) pilR 4069509..4070903 (+) 1395 WP_005930970.1 sigma-54 dependent transcriptional regulator Regulator
  XapA_RS18320 (XapA_18250) pilB 4071388..4071507 (-) 120 WP_005915819.1 hypothetical protein Machinery gene
  XapA_RS18330 (XapA_18260) pilB 4071820..4073556 (-) 1737 WP_174854896.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XapA_RS18335 (XapA_18265) pilA 4073619..4074053 (-) 435 WP_033480656.1 pilin Machinery gene
  XapA_RS18340 (XapA_18270) pilE 4074130..4074570 (-) 441 WP_005934296.1 pilin Machinery gene
  XapA_RS18345 (XapA_18275) pilC 4074897..4076153 (+) 1257 WP_033480657.1 type II secretion system F family protein Machinery gene
  XapA_RS18350 (XapA_18280) - 4076160..4077023 (+) 864 WP_005934302.1 A24 family peptidase -
  XapA_RS18355 (XapA_18285) coaE 4077037..4077645 (+) 609 WP_005934304.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62645.76 Da        Isoelectric Point: 5.6006

>NTDB_id=299788 XapA_RS18330 WP_174854896.1 4071820..4073556(-) (pilB) [Xanthomonas citri pv. punicae strain LMG7439]
MSVLSTANLVGITGIARRLVQDGALEESAARSAMDQAALAKVPLPQWFSEKKLVAASQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=299788 XapA_RS18330 WP_174854896.1 4071820..4073556(-) (pilB) [Xanthomonas citri pv. punicae strain LMG7439]
ATGAGCGTTTTATCGACCGCAAATCTAGTTGGAATCACCGGAATTGCTCGTAGGCTGGTGCAGGATGGCGCATTGGAGGA
AAGCGCGGCTCGAAGCGCGATGGATCAGGCTGCTCTAGCGAAAGTTCCACTGCCGCAGTGGTTCTCGGAGAAAAAGCTCG
TCGCTGCCTCGCAGTTGGCGGCCGCTAATGCAGTCGAGTTCGGCATGCCACTACTGGACGTATCGGCATTCGACGCCAAC
CAGAACGCAGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGTGCACTGGACGACATCAAGTTCCATACGAACCTAGTGGTCGAGGCGA
TCCTCGTTGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATGCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGAGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACCCTTGTCATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGAGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.786

100

0.559
  pilB Acinetobacter baylyi ADP1

54.922

100

0.55
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

46.277

97.578

0.452
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448

Multiple sequence alignment