Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DOM24_RS01195 Genome accession   NZ_CP030031
Coordinates   261690..263399 (-) Length   569 a.a.
NCBI ID   WP_005406017.1    Uniprot ID   -
Organism   Acinetobacter radioresistens strain LH6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256690..268399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOM24_RS01165 (DOM24_01165) - 256822..257145 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  DOM24_RS01170 (DOM24_01170) rlmB 257249..258004 (+) 756 WP_111280217.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DOM24_RS01175 (DOM24_01175) - 258029..258964 (+) 936 WP_101235097.1 DMT family transporter -
  DOM24_RS01180 (DOM24_01180) coaE 258961..259572 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  DOM24_RS01185 (DOM24_01185) pilD 259574..260434 (-) 861 WP_111280219.1 A24 family peptidase Machinery gene
  DOM24_RS01190 (DOM24_01190) pilC 260434..261660 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  DOM24_RS01195 (DOM24_01195) pilB 261690..263399 (-) 1710 WP_005406017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DOM24_RS01200 (DOM24_01200) tpiA 263688..264482 (+) 795 WP_005023075.1 triose-phosphate isomerase -
  DOM24_RS01205 (DOM24_01205) secG 264493..264822 (+) 330 WP_046206287.1 preprotein translocase subunit SecG -
  DOM24_RS01225 (DOM24_01225) rimP 265449..265973 (+) 525 WP_111280221.1 ribosome maturation factor RimP -
  DOM24_RS01230 (DOM24_01230) nusA 266014..267498 (+) 1485 WP_075041107.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63655.92 Da        Isoelectric Point: 5.0245

>NTDB_id=298562 DOM24_RS01195 WP_005406017.1 261690..263399(-) (pilB) [Acinetobacter radioresistens strain LH6]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAIRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNTPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=298562 DOM24_RS01195 WP_005406017.1 261690..263399(-) (pilB) [Acinetobacter radioresistens strain LH6]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAGTTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACCCTGTTTGTTGC
TACCAGTAATCCTACCAATATTGAAGCTTTAGAAGCTATCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATACGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTTGAGCCTTATGAAAAATATTACC
GGGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTTGCAACACCACCTTTACAGCTGGCTACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAAAC
CAAAGCCATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTAGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCGACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
CTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGTGCTCC
TGAAACACTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGATCTAGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTAAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.206

99.121

0.805
  pilB Acinetobacter baylyi ADP1

78.33

98.946

0.775
  pilB Legionella pneumophila strain ERS1305867

53.676

95.606

0.513
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

92.091

0.476
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50.287

91.916

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.516

100

0.376
  pilF Thermus thermophilus HB27

36.525

99.121

0.362

Multiple sequence alignment