Detailed information NTG0296276

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C9I47_RS04570
• Genome accession: NZ_CP029843
• Coordinates: 1016140..1017270 (+)
• Length: 376 a.a.
• NCBI ID: WP_111265700.1
• Uniprot ID: A0A2U9T1P9
• Organism: Marilutibacter maris strain HZ9B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1011140..1022270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C9I47_RS04545 (C9I47_0810)	-	1011571..1012371 (+)	801	WP_190238605.1	ABC transporter substrate-binding protein	-
C9I47_RS04550 (C9I47_0811)	-	1012408..1012929 (-)	522	WP_111265696.1	DUF4426 domain-containing protein	-
C9I47_RS04555 (C9I47_0812)	proC	1012939..1013790 (-)	852	WP_111265697.1	pyrroline-5-carboxylate reductase	-
C9I47_RS04560 (C9I47_0813)	-	1014098..1014826 (-)	729	WP_111265698.1	YggS family pyridoxal phosphate-dependent enzyme	-
C9I47_RS04565 (C9I47_0814)	pilT	1014996..1016033 (+)	1038	WP_111265699.1	type IV pilus twitching motility protein PilT	Machinery gene
C9I47_RS04570 (C9I47_0815)	pilU	1016140..1017270 (+)	1131	WP_111265700.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41775.05 Da
• Isoelectric Point: 6.6608

>NTDB_id=296276 C9I47_RS04570 WP_111265700.1 1016140..1017270(+) (pilU) [Marilutibacter maris strain HZ9B]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQSALTPQQSRDLVLNVMNPQQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELSLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDTWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQQGMKTF
DQSLFELYQAGEIGYEDALRYADSQNEVRLRIKLAQGGDAQTLAQGLDGVEVAEAR

Nucleotide

• Length: 1131 bp

>NTDB_id=296276 C9I47_RS04570 WP_111265700.1 1016140..1017270(+) (pilU) [Marilutibacter maris strain HZ9B]
ATGAGCACGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCGGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACCCAGAGCGCGCTGACCCCGCAGCAGAGCCGCGACCTCG
TGCTCAACGTGATGAACCCGCAGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGCGTTTCCGGT
GTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGTTGCGCCGGATCGAGACCAAGATTCC
GACCGTCGAGGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAC
CTGGGACGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTTGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GATCGCATCATCAACTTCTTCCCCGAGGATCGCCGCGGGCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGTGTGGT
CGCGCAGCAGCTGATTCCGACCCCCGACGGCAAGGCGCGCCGGGTCGCGATGGAAATCCTGCTCGGCACTCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCAGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCGGTTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGATGCCCAGACCCTGGCCCAGGGGCTGGATGGGGTCGAGGTGGCCG
AGGCGCGGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

3D structure

• AlphaFold DB: A0A2U9T1P9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.03	97.606	0.654
pilU	Acinetobacter baylyi ADP1	63.305	94.947	0.601
pilU	Vibrio cholerae strain A1552	52.809	94.681	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.571	93.085	0.396
pilT	Acinetobacter nosocomialis M2	40.407	91.489	0.37
pilT	Acinetobacter baumannii D1279779	40.407	91.489	0.37
pilT	Acinetobacter baumannii strain A118	40.407	91.489	0.37