Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C9I47_RS04380 Genome accession   NZ_CP029843
Coordinates   977225..978943 (-) Length   572 a.a.
NCBI ID   WP_111265667.1    Uniprot ID   -
Organism   Marilutibacter maris strain HZ9B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972225..983943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I47_RS04360 (C9I47_0775) - 972914..973864 (+) 951 WP_111265663.1 Nudix family hydrolase -
  C9I47_RS04365 (C9I47_0776) coaE 974085..974696 (-) 612 WP_111265664.1 dephospho-CoA kinase -
  C9I47_RS04370 (C9I47_0777) pilD 974790..975653 (-) 864 WP_111265665.1 A24 family peptidase Machinery gene
  C9I47_RS04375 (C9I47_0778) pilC 975657..976916 (-) 1260 WP_111265666.1 type II secretion system F family protein Machinery gene
  C9I47_RS04380 (C9I47_0779) pilB 977225..978943 (-) 1719 WP_111265667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C9I47_RS04385 (C9I47_0780) - 979078..979935 (+) 858 WP_407067497.1 glycosyltransferase family 2 protein -
  C9I47_RS04390 (C9I47_0781) - 979928..980557 (+) 630 WP_223250274.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  C9I47_RS04395 - 980554..981495 (+) 942 WP_111265669.1 lysylphosphatidylglycerol synthase domain-containing protein -
  C9I47_RS04400 (C9I47_0782) - 981514..983463 (-) 1950 WP_111265670.1 hypothetical protein -
  C9I47_RS04405 (C9I47_0783) - 983533..983943 (-) 411 WP_111265671.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62388.58 Da        Isoelectric Point: 5.0850

>NTDB_id=296274 C9I47_RS04380 WP_111265667.1 977225..978943(-) (pilB) [Marilutibacter maris strain HZ9B]
MNTVTTANLIGITGIARRLVLDGALDEASAREAMESATAEKKPLAAFLAEKRLVSQPALAAANSIEFGMPLFDPGALDPA
QSAVKLVSEELIRKHNVLPLFKRGNRLFVGIADPTNIHALDEIKFQSNLVVDAILIDEESIKRNLDRWLDSADVMEGGDD
EGLENLEVGSGEDDAGTDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQIAKVPVKLQA
RIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEDDQKQLYLEA
IQKPYGMVLVTGPTGSGKTVSLYTALNILNDDTRNISTVEDPVEIRVPGINQVQMNIKRGMTFAAALRSFLRQDPDVIMV
GEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVIAQRLARRLHDCKRKVELPEHALLA
EGFTQEEIDAGLTLYDAVGCPDCTEGYKGRTGIYQVMPMSEEIQEIVLAGGNALQIAEAAQKAGINDLRQSALLKVRNGV
TSLAEINRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=296274 C9I47_RS04380 WP_111265667.1 977225..978943(-) (pilB) [Marilutibacter maris strain HZ9B]
ATGAACACCGTCACGACCGCCAATCTCATCGGCATTACCGGCATCGCCCGACGCCTGGTCCTGGACGGGGCCCTCGACGA
AGCGAGTGCCCGCGAGGCGATGGAATCGGCCACCGCCGAGAAGAAGCCGCTGGCGGCCTTCCTCGCCGAGAAGCGGCTGG
TCTCCCAGCCGGCGCTGGCCGCCGCCAACTCGATCGAGTTCGGCATGCCGCTGTTCGACCCCGGCGCGCTCGACCCGGCC
CAGTCGGCGGTCAAGCTGGTCAGCGAGGAGCTGATCCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
GTTCGTCGGCATCGCCGACCCGACCAACATCCATGCGCTGGACGAGATCAAGTTCCAGAGCAATCTGGTGGTGGACGCGA
TCCTGATCGACGAGGAGTCGATCAAGCGCAATCTCGACCGCTGGCTGGACAGCGCCGACGTCATGGAGGGCGGCGACGAC
GAGGGCCTGGAGAACCTGGAGGTCGGCAGCGGCGAGGACGATGCCGGCACCGACTCCGGTGTCGACGCCAAGGGTGACGA
CACCCCGGTCGTGAAGTTCGTCAACAAGGTACTGGTCGACGCGATCCGCCGCGGCGCCTCGGACATCCACTTCGAACCCT
ACGAGACCGAGTACCGGGTCCGCCTGCGCATCGACGGCCTGCTCAAGCAGATCGCCAAGGTCCCGGTCAAGCTGCAGGCC
CGCATCGCCGCGCGCCTGAAGGTGATGTCCCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGCCGCATCAAGCT
GAACCTGTCCAAGAGCCGGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTTGGCGAGAAGATCGTGCTGCGTA
TCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGTTACGAGGACGACCAGAAGCAGCTGTACCTGGAGGCG
ATCCAGAAGCCGTACGGCATGGTCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGCGCTGAA
CATCCTCAACGACGACACCCGCAACATCTCCACCGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCAACCAGGTGC
AGATGAACATCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTGATCATGGTC
GGCGAGATCCGCGACCTGGAGACTGCCGAGATCGGCATCAAGGCCGCCCAGACCGGTCACATGGTGTTGTCGACCCTGCA
CACCAACGACGCACCGCAGACCATCGCCCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCACCTCATCGGTAACCC
TGGTGATTGCCCAGCGCCTGGCCCGGCGCCTGCACGACTGCAAGCGCAAGGTCGAGCTGCCCGAGCACGCACTGCTGGCC
GAGGGCTTCACCCAGGAGGAGATCGACGCCGGGCTGACCCTGTACGACGCGGTCGGCTGCCCCGACTGCACCGAAGGCTA
CAAGGGCCGTACCGGCATCTACCAGGTGATGCCGATGAGCGAGGAGATCCAGGAGATCGTGCTGGCCGGCGGCAACGCGC
TGCAGATCGCCGAGGCGGCGCAGAAGGCCGGCATCAACGACCTGCGCCAGTCGGCCCTGCTCAAGGTCAGGAACGGGGTC
ACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.773

98.427

0.549
  pilB Acinetobacter baylyi ADP1

55.398

98.776

0.547
  pilB Legionella pneumophila strain ERS1305867

52.028

99.126

0.516
  pilB Vibrio cholerae strain A1552

52.827

89.685

0.474
  pilF Neisseria gonorrhoeae MS11

48.135

98.427

0.474
  pilB Vibrio campbellii strain DS40M4

51.072

89.685

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

50.884

88.986

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

39.773

92.308

0.367
  pilF Thermus thermophilus HB27

40.504

90.21

0.365

Multiple sequence alignment