Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DLE54_RS00810 Genome accession   NZ_CP029789
Coordinates   190351..191613 (-) Length   420 a.a.
NCBI ID   WP_110815937.1    Uniprot ID   -
Organism   Psychrobacter sp. YP14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 185351..196613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLE54_RS00785 (DLE54_00785) - 185389..185721 (+) 333 WP_011959442.1 pyrimidine/purine nucleoside phosphorylase -
  DLE54_RS00790 (DLE54_00790) rlmB 185924..186709 (+) 786 WP_011959443.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DLE54_RS00795 (DLE54_00795) coaE 186813..187520 (-) 708 WP_083757352.1 dephospho-CoA kinase -
  DLE54_RS00800 (DLE54_00800) - 188044..188964 (-) 921 WP_197708894.1 prepilin peptidase -
  DLE54_RS00805 (DLE54_00805) pilC 189048..190208 (-) 1161 WP_227535697.1 type II secretion system F family protein Machinery gene
  DLE54_RS00810 (DLE54_00810) pilB 190351..191613 (-) 1263 WP_110815937.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DLE54_RS00815 (DLE54_00815) tpiA 191949..192746 (+) 798 WP_110815938.1 triose-phosphate isomerase -
  DLE54_RS00820 (DLE54_00820) secG 192912..193229 (+) 318 WP_110815939.1 preprotein translocase subunit SecG -
  DLE54_RS00835 (DLE54_00835) rimP 194186..194686 (+) 501 WP_011959450.1 ribosome maturation factor RimP -
  DLE54_RS00840 (DLE54_00840) nusA 194978..196465 (+) 1488 WP_011959451.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45740.12 Da        Isoelectric Point: 6.5632

>NTDB_id=296034 DLE54_RS00810 WP_110815937.1 190351..191613(-) (pilB) [Psychrobacter sp. YP14]
MSRPPSLNQLAAAPSATLAQAEHGAHQPHDLQQDPVVQLVQDLLCQAVDKGASDLHFEPYEHTFRVRFRIDGVMQQVATP
ALELQAKICARLKIMAQMDISERRMPQDGRIRLQLNGMPTLNFRANALPTLFGEKIVLRVINPAASIAGIDSLGLEPEQK
QQFLQALQQPQGILLITGPTGSGKTLSLYTGLDILNTVATNICTVEDPVEINLTGINQVTVNPKVGLSFAVALKSFLRQD
PDVLMVGEIRDFETADTAIKAAQTGHLVLSTLHTNSAAETLTRLHHMGVAYFNIATAISLVVAQRLARCLCRHCKIPCVV
PEVSLIEIGFSAEELAQPEHIIYKAVGCDKCCEGYKGRIGVFEVMPMNTALAQVIMQGANAIEIKAAATEQGFFDLRRAA
ILKVLQGILSIEEMTRMTAQ

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=296034 DLE54_RS00810 WP_110815937.1 190351..191613(-) (pilB) [Psychrobacter sp. YP14]
ATGTCCCGGCCCCCTTCGCTTAATCAACTAGCGGCCGCTCCCTCTGCGACTCTAGCACAGGCAGAGCATGGCGCGCATCA
GCCTCATGATTTACAGCAAGATCCGGTAGTTCAGCTGGTACAGGATTTATTGTGTCAGGCAGTAGATAAAGGCGCCTCTG
ACCTACACTTTGAGCCCTATGAGCATACGTTTCGGGTACGCTTTCGTATCGATGGCGTCATGCAGCAAGTGGCCACACCA
GCGCTTGAGCTGCAAGCTAAGATATGTGCCCGCCTAAAGATTATGGCACAAATGGACATATCTGAGCGGCGTATGCCTCA
AGATGGCCGTATTAGGCTGCAACTAAATGGGATGCCGACACTAAACTTTCGTGCCAATGCTCTACCCACTTTGTTCGGTG
AAAAAATTGTGCTTCGGGTTATTAATCCAGCCGCCTCTATCGCTGGTATTGATAGCCTCGGGCTTGAGCCTGAGCAAAAA
CAGCAGTTTTTACAAGCGCTACAGCAGCCGCAAGGTATCTTATTAATTACCGGCCCCACAGGCTCTGGCAAAACCTTATC
GCTCTATACCGGTCTAGATATTTTAAACACGGTGGCCACCAATATCTGCACTGTAGAAGATCCGGTTGAAATTAATTTAA
CAGGTATCAATCAGGTCACCGTCAATCCTAAAGTCGGACTGAGCTTTGCGGTGGCCTTAAAGTCATTTTTGCGCCAAGAC
CCCGATGTATTAATGGTGGGTGAGATACGTGATTTTGAAACCGCGGATACTGCAATTAAAGCGGCACAAACTGGCCATTT
GGTGCTCTCTACCCTGCACACCAACTCAGCTGCTGAAACCTTAACTCGCCTGCATCATATGGGCGTAGCTTACTTTAATA
TTGCTACCGCCATTAGTCTGGTAGTGGCGCAGCGTCTGGCAAGATGCTTGTGCCGCCACTGCAAAATCCCTTGTGTGGTT
CCTGAGGTAAGTCTCATTGAGATTGGCTTTAGCGCCGAAGAGTTGGCACAGCCTGAGCATATCATCTATAAGGCGGTGGG
CTGTGATAAGTGCTGTGAGGGTTACAAAGGGCGCATCGGTGTGTTTGAGGTGATGCCCATGAACACGGCGCTTGCACAAG
TGATTATGCAAGGTGCTAATGCCATTGAGATAAAAGCGGCTGCCACCGAGCAAGGCTTTTTTGACTTAAGGCGGGCCGCT
ATTTTGAAAGTGCTTCAAGGCATCCTTAGCATTGAAGAAATGACCCGCATGACGGCCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-308)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

62.694

91.905

0.576
  pilB Acinetobacter baumannii D1279779

60.622

91.905

0.557
  pilB Vibrio cholerae strain A1552

55.216

93.571

0.517
  pilF Neisseria gonorrhoeae MS11

55.243

93.095

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

53.061

93.333

0.495
  pilB Legionella pneumophila strain ERS1305867

53.766

91.667

0.493
  pilB Vibrio campbellii strain DS40M4

52.284

93.81

0.49
  pilB/pilB1 Synechocystis sp. PCC 6803

43.415

97.619

0.424
  pilF Thermus thermophilus HB27

42.68

95.952

0.41
  pilB Haemophilus influenzae Rd KW20

40.338

98.571

0.398
  pilB Haemophilus influenzae 86-028NP

40.097

98.571

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.636

92.143

0.393

Multiple sequence alignment