Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DLK06_RS18875 Genome accession   NZ_CP029610
Coordinates   3845974..3847686 (+) Length   570 a.a.
NCBI ID   WP_032070753.1    Uniprot ID   A0AAE9M8P4
Organism   Acinetobacter pittii strain ST220     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3840974..3852686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLK06_RS18840 (DLK06_18860) nusA 3841862..3843346 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  DLK06_RS18845 (DLK06_18865) rimP 3843384..3843908 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  DLK06_RS18865 (DLK06_18885) secG 3844546..3844875 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  DLK06_RS18870 (DLK06_18890) tpiA 3844888..3845682 (-) 795 WP_032063691.1 triose-phosphate isomerase -
  DLK06_RS18875 (DLK06_18895) pilB 3845974..3847686 (+) 1713 WP_032070753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DLK06_RS18880 (DLK06_18900) pilC 3847713..3848939 (+) 1227 WP_126117775.1 type II secretion system F family protein Machinery gene
  DLK06_RS18885 (DLK06_18905) pilD 3848939..3849799 (+) 861 WP_032070754.1 prepilin peptidase Machinery gene
  DLK06_RS18890 (DLK06_18910) coaE 3849801..3850397 (+) 597 WP_032070755.1 dephospho-CoA kinase -
  DLK06_RS18895 (DLK06_18915) - 3850394..3851308 (-) 915 WP_032070756.1 DMT family transporter -
  DLK06_RS18900 (DLK06_18920) rlmB 3851344..3852093 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DLK06_RS18905 (DLK06_18925) - 3852196..3852522 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63526.90 Da        Isoelectric Point: 5.0911

>NTDB_id=294204 DLK06_RS18875 WP_032070753.1 3845974..3847686(+) (pilB) [Acinetobacter pittii strain ST220]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPASQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=294204 DLK06_RS18875 WP_032070753.1 3845974..3847686(+) (pilB) [Acinetobacter pittii strain ST220]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGATCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAACAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCAGCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGACGGCGTGTTACGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCAACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGATCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.088

100

0.951
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment