Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DLD99_RS02345 Genome accession   NZ_CP029608
Coordinates   496221..498299 (+) Length   692 a.a.
NCBI ID   WP_114881132.1    Uniprot ID   A0A345RJ86
Organism   Pseudomonas kribbensis strain 46-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491221..503299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLD99_RS02325 (DLD99_02325) pilM 493436..494500 (+) 1065 WP_114881130.1 pilus assembly protein PilM Machinery gene
  DLD99_RS02330 (DLD99_02330) - 494500..495066 (+) 567 WP_085711056.1 PilN domain-containing protein -
  DLD99_RS02335 (DLD99_02335) pilO 495063..495686 (+) 624 WP_085711057.1 type 4a pilus biogenesis protein PilO -
  DLD99_RS02340 (DLD99_02340) - 495683..496207 (+) 525 WP_114881131.1 pilus assembly protein PilP -
  DLD99_RS02345 (DLD99_02345) pilQ 496221..498299 (+) 2079 WP_114881132.1 type IV pilus secretin PilQ Machinery gene
  DLD99_RS02350 (DLD99_02350) aroK 498304..498822 (+) 519 WP_011332081.1 shikimate kinase AroK -
  DLD99_RS02355 (DLD99_02355) aroB 498965..500065 (+) 1101 WP_114881133.1 3-dehydroquinate synthase -
  DLD99_RS02360 (DLD99_02360) - 500076..501674 (+) 1599 WP_114881134.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75119.30 Da        Isoelectric Point: 5.5565

>NTDB_id=294060 DLD99_RS02345 WP_114881132.1 496221..498299(+) (pilQ) [Pseudomonas kribbensis strain 46-2]
MNRIFSTLGFSLWIALLSPMVHAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLATKTRD
LGSGNARSATVVEANDRTRLIINLTQLAPYDARVEGNTLFVVVGQGTKASAPRPVAKTTRATPVAAPARTYAPVARAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPGGT
FDYSTYQTDNKLTVSIRPLTIDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=294060 DLD99_RS02345 WP_114881132.1 496221..498299(+) (pilQ) [Pseudomonas kribbensis strain 46-2]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCACGCGGCCAACCTGAA
GACGCTGGACGTTGCCGCGCTGCCGGGTGATCGCGTCGAGCTGAAGCTGTCGTTCGACGGCCCGCCGCCGCAGCCCAAGG
GTTACACCACCGACTCGCCGGCGCGGATCGCCCTCGACCTGCCGGGAGTGGCCAGCCAGCTCGCGACCAAGACCCGCGAC
CTCGGTAGCGGCAATGCGCGCTCTGCCACGGTGGTCGAGGCCAATGACCGTACCCGGTTGATCATCAACCTGACGCAACT
GGCGCCGTATGACGCCCGGGTCGAGGGCAACACGCTGTTCGTGGTGGTCGGGCAGGGCACCAAGGCCAGCGCACCGCGAC
CTGTCGCGAAGACAACGCGTGCCACGCCGGTGGCAGCGCCGGCGCGGACTTACGCCCCCGTCGCCAGGGCCATTCGTGGT
GTGGATTTCCAGCGCGGCACGGCGGGTGAGGGCAATGTGGTCATCGACCTGTCCGACCCGACCATCGCGCCGGACATTCA
GGAGCACGACGGCAAGATCATCCTCAATTTCGCCCGCACCCAGTTGCCCGAGCCGTTGCGGGTGCGTCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAACGCCGGCGTGAGCGGGGATCGGGCGACCATCACTGTAGAGCCCGGCGGCACG
TTCGACTATTCCACCTACCAGACCGACAACAAGCTGACGGTGAGCATCCGTCCGCTGACCATCGACGACCTGCAAAAGCG
CAATGCCGACCGCAACAGCTACAGCGGTGAAAAGCTTTCGTTGAATTTCCAGGACATCGATGTGCGTTCGGTACTGCAAC
TGATCGCGGACTTCACCAATCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGGATCACCTTGCGCCTGCAAAACGTA
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAACGCAAGGTCGGCAACGTGTTGCTGGTAGC
ACCGGCGGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCGCTGCGCC
GTGAACTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCGAAG
GTCGATGAACGCGGCTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATCGTGGCGCAGCTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCCTGGGTGTGCGCTGGGGCGGGTCAATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
ATCCGGGATCGGCATTGCGTTCATCACCGACAACGTTCTGCTGGACCTTGAACTGACGGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAACCCAAGGTGGTCACCTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATCAC
CCCGGACAATCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGTGGGGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345RJ86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  pilQ Acinetobacter baumannii strain A118

38.051

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.051

100

0.412
  comQ Acinetobacter baylyi ADP1

40.922

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.37

97.543

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.187

97.254

0.371

Multiple sequence alignment