Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DLD99_RS02325 Genome accession   NZ_CP029608
Coordinates   493436..494500 (+) Length   354 a.a.
NCBI ID   WP_114881130.1    Uniprot ID   A0A345RJ82
Organism   Pseudomonas kribbensis strain 46-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488436..499500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLD99_RS02315 (DLD99_02315) - 489336..490604 (+) 1269 WP_085711053.1 malic enzyme-like NAD(P)-binding protein -
  DLD99_RS02320 (DLD99_02320) - 490772..493219 (-) 2448 WP_085711054.1 penicillin-binding protein 1A -
  DLD99_RS02325 (DLD99_02325) pilM 493436..494500 (+) 1065 WP_114881130.1 pilus assembly protein PilM Machinery gene
  DLD99_RS02330 (DLD99_02330) - 494500..495066 (+) 567 WP_085711056.1 PilN domain-containing protein -
  DLD99_RS02335 (DLD99_02335) pilO 495063..495686 (+) 624 WP_085711057.1 type 4a pilus biogenesis protein PilO -
  DLD99_RS02340 (DLD99_02340) - 495683..496207 (+) 525 WP_114881131.1 pilus assembly protein PilP -
  DLD99_RS02345 (DLD99_02345) pilQ 496221..498299 (+) 2079 WP_114881132.1 type IV pilus secretin PilQ Machinery gene
  DLD99_RS02350 (DLD99_02350) aroK 498304..498822 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38021.50 Da        Isoelectric Point: 4.7582

>NTDB_id=294059 DLD99_RS02325 WP_114881130.1 493436..494500(+) (pilM) [Pseudomonas kribbensis strain 46-2]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLAKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVGEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=294059 DLD99_RS02325 WP_114881130.1 493436..494500(+) (pilM) [Pseudomonas kribbensis strain 46-2]
GTGCTAGGACTCTTCAATAAAAAGACCAATACGTTGCTGGGGATCGATATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGTCGTCAGGGCGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCCAATGCCGTGGTCGAGAAGAACA
TCGCCGAGCTTGAAGGCGTCGGGCATGCCCTGTCCCGGGTACTGGCCAAGGCGCGCACCGGGCTCAAAAGCGTGGCGGTG
GCAGTGGCCGGCTCGGCGGTGATCACCAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTACCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAATCCCGAGCGGGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAGGCGGCGCTGGCCCTG
GCCGGCCTGACGGCGCGGGTGGTGGATGTCGAGGCGTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
GGCCTCGCAGGAGCGCCTGACCGTTGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAGCAACTGTTCGGCGGGCGCCAGCTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCCTC
GAACAGGCCGGGCTGGCCAAGAAACAGGGCGGTCTGCCGGACGATTATGTCGGCGAGGTCCTGCAGCCGTTTCGCGAGGC
GCTGGTGCAACAGGTGTCGCGTTCCCTGCAATTTTTCTTTGCTTCCGGCCAGTACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCCTCGGTGCCGGGGCTGGACCGGTTGATCGAAGAGCGCCTCGGCACCCCGACCCAGGTCGCCAATCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345RJ82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489

Multiple sequence alignment