Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DKK66_RS04390 Genome accession   NZ_CP029539
Coordinates   933985..935697 (-) Length   570 a.a.
NCBI ID   WP_131355826.1    Uniprot ID   A0A4P6THV0
Organism   Aquitalea sp. USM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928985..940697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKK66_RS04365 (DKK66_04355) yacG 930270..930467 (-) 198 WP_045845412.1 DNA gyrase inhibitor YacG -
  DKK66_RS04370 (DKK66_04360) zapD 930473..931231 (-) 759 WP_045845413.1 cell division protein ZapD -
  DKK66_RS04375 (DKK66_04365) coaE 931271..931960 (-) 690 WP_370302793.1 dephospho-CoA kinase -
  DKK66_RS04380 (DKK66_04370) pilD 931872..932741 (-) 870 WP_131355821.1 A24 family peptidase Machinery gene
  DKK66_RS04385 (DKK66_04375) pilC 932744..933964 (-) 1221 WP_131355824.1 type II secretion system F family protein Machinery gene
  DKK66_RS04390 (DKK66_04380) pilF 933985..935697 (-) 1713 WP_131355826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DKK66_RS04395 (DKK66_04385) - 935882..936952 (-) 1071 WP_131355829.1 porin -
  DKK66_RS04400 (DKK66_04390) - 937294..938691 (+) 1398 WP_131355832.1 deoxyribodipyrimidine photo-lyase -
  DKK66_RS04405 (DKK66_04395) comE 938748..939083 (-) 336 WP_045845420.1 ComEA family DNA-binding protein Machinery gene
  DKK66_RS04410 (DKK66_04400) - 939121..939942 (-) 822 WP_062786365.1 inner membrane protein YpjD -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62085.50 Da        Isoelectric Point: 5.2071

>NTDB_id=293348 DKK66_RS04390 WP_131355826.1 933985..935697(-) (pilF) [Aquitalea sp. USM4]
MEASAGISGLGRALVQNNALLLQDAEAIQRQAQASGISFVEQLIASKKMSAVEVATFASGVFGYPLLDMGAIDPVQLPKG
LVDDELIGSRRLVPLFKRGNKLFIGTSDPTQTSAFHAITFKTGLTVEVVVVEDDKLSKVIGKYTEDATAAIKALENEEFG
DLEFVDPDEPTPHSETNQPDVDDAPVVKFIHKVLLDAINGGASDVHFEPYEKFYRIRYRVDGQLKEIAQPPLLLKEKIAS
RIKVISRLDISEKRVPQDGRLKLVLSKTHSIDFRVSTLPTLFGEKIVMRILDASAASLDIEQLGFEPEQKKMLLEAIARP
YGMVLVTGPTGSGKTVSLYTCLNILNKPDINISTAEDPVEINLPGINQVNVNEKAGLTFSSALKAFLRQDPDVIMVGEIR
DFETADISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNVASSVLLIMAQRLARRLCNHCKAPIDIPEPALLRAGF
RPEELDGSWKPYGPVGCDECRGSGYKGRTGIYEVMPISDAMTRLIMQNGTAIDISDLALQEGMIDLRHAGLLKVKRGLTS
LAEIEAVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=293348 DKK66_RS04390 WP_131355826.1 933985..935697(-) (pilF) [Aquitalea sp. USM4]
ATGGAAGCTTCTGCTGGGATATCTGGTCTGGGAAGAGCGCTGGTGCAGAACAATGCATTGCTGTTACAGGATGCAGAGGC
TATCCAGCGTCAGGCGCAGGCATCTGGCATCAGTTTTGTCGAGCAATTGATTGCCAGCAAGAAGATGTCCGCAGTGGAGG
TTGCCACTTTTGCGTCGGGTGTATTTGGTTATCCTTTGTTGGACATGGGTGCCATCGATCCTGTTCAGCTGCCCAAAGGG
CTGGTTGATGATGAATTGATTGGCAGTCGTCGTCTGGTTCCTTTGTTCAAGCGTGGTAACAAGCTGTTTATTGGTACGTC
TGATCCGACCCAGACATCCGCGTTCCACGCCATTACTTTCAAGACCGGTCTGACGGTTGAAGTGGTCGTGGTTGAAGATG
ACAAACTTTCCAAAGTGATTGGGAAGTACACCGAAGATGCAACTGCTGCGATCAAGGCCTTGGAGAACGAGGAGTTTGGT
GATCTGGAGTTTGTTGATCCGGATGAGCCCACTCCGCACAGTGAAACCAATCAGCCTGATGTCGATGACGCTCCTGTCGT
CAAGTTCATTCACAAGGTTCTGCTGGACGCCATTAACGGAGGGGCATCCGACGTCCATTTCGAGCCTTACGAAAAGTTTT
ACCGCATTCGTTATCGTGTGGATGGCCAGCTGAAAGAGATTGCGCAGCCGCCTTTGCTGTTGAAAGAAAAAATCGCGTCC
CGTATCAAGGTGATATCGCGCTTGGATATTTCCGAGAAGCGCGTACCGCAGGATGGTCGTTTGAAGCTGGTGTTGTCCAA
GACACATTCAATTGATTTTCGTGTCAGTACCTTGCCAACCCTGTTTGGCGAGAAGATCGTAATGCGTATTCTGGATGCTT
CTGCCGCATCACTGGATATCGAACAGCTGGGTTTTGAACCAGAGCAGAAGAAAATGCTGCTCGAAGCCATTGCGCGTCCA
TACGGGATGGTGCTGGTAACCGGGCCTACCGGTAGTGGTAAAACGGTTTCGCTTTATACTTGCCTTAATATCCTGAACAA
GCCGGATATCAATATTTCGACGGCGGAGGATCCGGTTGAAATCAATTTGCCTGGCATTAACCAGGTCAATGTAAACGAGA
AGGCTGGTCTCACCTTCTCGTCTGCCTTGAAAGCGTTTCTGCGGCAGGATCCTGATGTCATCATGGTGGGTGAAATCCGG
GATTTCGAAACGGCAGACATTTCCATCAAGGCCGCACAAACCGGCCACATGGTGTTTTCCACCTTGCATACCAATAATGC
GCCTGCCACCTTGACTCGTTTGCTGAACATGGGTGTGGCACCATTCAACGTAGCCAGTTCGGTACTGTTGATCATGGCCC
AGCGTCTGGCCAGACGCTTGTGCAACCACTGCAAGGCACCGATCGATATTCCTGAACCGGCATTGCTGCGGGCGGGTTTC
CGCCCGGAGGAACTGGATGGCAGCTGGAAACCATACGGTCCTGTTGGCTGCGACGAATGCCGTGGCTCGGGCTATAAAGG
ACGTACCGGTATTTATGAAGTCATGCCCATCAGTGATGCCATGACCCGTCTGATCATGCAGAATGGTACTGCCATTGATA
TCAGTGACCTTGCCTTGCAAGAGGGCATGATTGATTTGCGTCATGCCGGTTTGCTGAAGGTAAAGCGTGGGCTGACCTCG
CTGGCCGAGATCGAGGCTGTGACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6THV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.143

99.474

0.568
  pilB Acinetobacter baumannii D1279779

53.633

100

0.544
  pilB Acinetobacter baylyi ADP1

54.594

99.298

0.542
  pilB Legionella pneumophila strain ERS1305867

52.557

99.474

0.523
  pilB Vibrio cholerae strain A1552

51.095

96.14

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

48.05

98.947

0.475
  pilB Vibrio campbellii strain DS40M4

46.726

99.123

0.463
  pilF Thermus thermophilus HB27

37.986

99.298

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.091

96.491

0.377

Multiple sequence alignment