Detailed information NTG0291424

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: DG474_RS06020
• Genome accession: NZ_CP029257
• Coordinates: 1224452..1225102 (-)
• Length: 216 a.a.
• NCBI ID: WP_125397719.1
• Uniprot ID: A0A3R9JLF8
• Organism: Streptococcus oralis strain CCUG 53468
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1219452..1230102

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DG474_RS06000 (DG474_06110)	rplT	1220834..1221193 (-)	360	WP_000124830.1	50S ribosomal protein L20	-
DG474_RS06005 (DG474_06115)	rpmI	1221245..1221445 (-)	201	WP_001125942.1	50S ribosomal protein L35	-
DG474_RS06010 (DG474_06120)	infC	1221478..1222008 (-)	531	WP_000848184.1	translation initiation factor IF-3	-
DG474_RS06015 (DG474_06125)	comEC/celB	1222228..1224468 (-)	2241	WP_200371573.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DG474_RS06020 (DG474_06130)	comEA/celA/cilE	1224452..1225102 (-)	651	WP_125397719.1	helix-hairpin-helix domain-containing protein	Machinery gene
DG474_RS06025 (DG474_06135)	-	1225169..1225738 (-)	570	WP_255777670.1	GNAT family N-acetyltransferase	-
DG474_RS06030 (DG474_06145)	ald	1225914..1227026 (+)	1113	WP_255777671.1	alanine dehydrogenase	-
DG474_RS06035 (DG474_06150)	-	1227077..1228063 (-)	987	WP_084921953.1	PhoH family protein	-
DG474_RS06040 (DG474_06155)	-	1228144..1228359 (-)	216	WP_001232084.1	YozE family protein	-
DG474_RS06045 (DG474_06160)	-	1228356..1228961 (-)	606	WP_255777672.1	GrpB family protein	-
DG474_RS06050 (DG474_06165)	cvfB	1228973..1229827 (-)	855	WP_071850841.1	RNA-binding virulence regulatory protein CvfB	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23105.27 Da
• Isoelectric Point: 5.9212

>NTDB_id=291424 DG474_RS06020 WP_125397719.1 1224452..1225102(-) (comEA/celA/cilE) [Streptococcus oralis strain CCUG 53468]
MEAIIEKIKEYKIIVICACLGLALGGFFLLKPTSQTSVKETNLQAEVAAVSKDSSSEKEIKKEEKDESAEQDLITVDVKG
AVKSPGIYDLPVGSRVHDAVQKAGGLTDEADSKSLNLAQKVSDEALVYVPTKGEEAASQQAASGTTPSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTAD

Nucleotide

• Length: 651 bp

>NTDB_id=291424 DG474_RS06020 WP_125397719.1 1224452..1225102(-) (comEA/celA/cilE) [Streptococcus oralis strain CCUG 53468]
ATGGAAGCAATTATCGAGAAAATAAAAGAGTATAAAATCATTGTCATCTGTGCTTGTTTGGGTTTGGCCTTGGGCGGATT
TTTCCTACTAAAGCCAACTTCACAAACATCTGTGAAAGAAACAAACTTGCAGGCTGAAGTTGCAGCTGTTTCAAAGGATT
CATCATCTGAAAAGGAAATCAAGAAGGAAGAAAAGGATGAGTCTGCTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTTAAATCGCCAGGAATTTATGATTTACCAGTAGGGAGTCGTGTTCATGATGCCGTTCAGAAGGCAGGTGGCTTGAC
AGATGAGGCAGATAGCAAGTCGCTCAATCTAGCACAGAAAGTCAGTGACGAGGCTCTTGTCTATGTTCCAACTAAGGGAG
AAGAAGCAGCTAGTCAGCAGGCTGCCTCTGGAACGACTCCTTCGACAAGTAAAGAAAAGAAAGTCAACCTCAATAAAGCT
AGTCTGGAAGAACTAAAGCAGGTCAAAGGCTTGGGAGGAAAACGAGCCCAGGATATTATCGACCATCGTGAGGCAAATGG
CAAATTCAAGTCGGTTGATGAATTAAAGAAGGTCTCTGGTATTGGCGCTAAGACCATAGAAAAGCTAAAAGATTATGTCA
CAGCGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A3R9JLF8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	90.278	100	0.903
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	89.815	100	0.898
comEA/celA/cilE	Streptococcus pneumoniae D39	87.963	100	0.88
comEA/celA/cilE	Streptococcus pneumoniae R6	87.963	100	0.88
comEA/celA/cilE	Streptococcus pneumoniae Rx1	87.963	100	0.88
comEA/celA/cilE	Streptococcus mitis SK321	85.648	100	0.856
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Staphylococcus aureus N315	35.294	100	0.361
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361