Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DDU33_RS09255 Genome accession   NZ_CP029206
Coordinates   1918605..1919990 (-) Length   461 a.a.
NCBI ID   WP_108924837.1    Uniprot ID   A0A2U8FMQ4
Organism   Actinobacillus porcitonsillarum strain 9953L55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1913605..1924990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDU33_RS09225 (DDU33_09225) - 1914046..1914675 (-) 630 WP_108924831.1 hypothetical protein -
  DDU33_RS09230 (DDU33_09230) - 1914856..1915818 (-) 963 WP_108924832.1 helix-turn-helix transcriptional regulator -
  DDU33_RS09235 (DDU33_09235) yacG 1915927..1916112 (-) 186 WP_108924833.1 DNA gyrase inhibitor YacG -
  DDU33_RS09240 (DDU33_09240) coaE 1916094..1916726 (-) 633 WP_108924834.1 dephospho-CoA kinase -
  DDU33_RS09245 (DDU33_09245) - 1916752..1917384 (-) 633 WP_244175317.1 prepilin peptidase -
  DDU33_RS09250 (DDU33_09250) pilC 1917416..1918612 (-) 1197 WP_108924836.1 type II secretion system F family protein Machinery gene
  DDU33_RS09255 (DDU33_09255) pilB 1918605..1919990 (-) 1386 WP_108924837.1 GspE/PulE family protein Machinery gene
  DDU33_RS09260 (DDU33_09260) pilA 1920040..1920486 (-) 447 WP_005823732.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DDU33_RS09265 (DDU33_09265) radA 1920672..1922051 (+) 1380 WP_108924838.1 DNA repair protein RadA -
  DDU33_RS09270 (DDU33_09270) - 1922086..1922571 (-) 486 WP_108924839.1 dihydrofolate reductase -
  DDU33_RS09275 (DDU33_09275) proB 1922694..1923785 (+) 1092 WP_108924840.1 glutamate 5-kinase -
  DDU33_RS09280 (DDU33_09280) - 1923809..1924573 (+) 765 WP_108924841.1 TSUP family transporter -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52752.76 Da        Isoelectric Point: 5.9906

>NTDB_id=291146 DDU33_RS09255 WP_108924837.1 1918605..1919990(-) (pilB) [Actinobacillus porcitonsillarum strain 9953L55]
MTYSVCDLNTQEVFDISEKQWQKNCHEKQILLRYLAVPIKEDKEKLWLAIDDLNNLTACEIFSFLVHKAIEPVLISREEL
KYLLNELSPEQQVVYEESEFQLAQKDQEEIINPNDPIIQTLDQLFKFCLQQNASDIHIEPQKAHLMIRLRIDGVLHIYKT
LPLNLSNRIVSRIKLLAKLDISETRFPQDGQFHFKTLLGDVLDFRVSSLPTNYGEKLVLRLQKNKPTQADFLSLGFNQHQ
KEKLIFALEQPQGLILVTGPTGSGKSITLYSALSYLNNNQRHIITAEDPIEIEIEGIIQTQVNRGIQLDFSQLLRTFLRQ
DPDIIMFGEIRDEESAQMALRAAQTGHLVLSTLHTNDAFSAVERLLQLGIKEYELNNSLLLVIAQRLVRTLCPSCLGKGC
EHCYQGYKGRTGVYQLLSKNGNFFEKTTACLDFKTLAESAKEKVMAKITDETEVKRVLGYV

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=291146 DDU33_RS09255 WP_108924837.1 1918605..1919990(-) (pilB) [Actinobacillus porcitonsillarum strain 9953L55]
ATGACTTATTCAGTATGTGATCTTAATACTCAAGAAGTATTTGATATTTCAGAAAAACAGTGGCAGAAAAATTGCCATGA
AAAGCAGATATTGCTTCGTTATTTAGCGGTGCCAATAAAAGAAGATAAAGAAAAACTTTGGTTAGCCATTGATGATTTAA
ATAATTTAACCGCTTGCGAAATTTTCTCATTTTTGGTGCATAAAGCCATTGAGCCCGTATTAATTTCTAGGGAAGAGTTA
AAGTATTTACTTAATGAATTATCGCCTGAACAACAAGTAGTTTATGAAGAATCAGAATTTCAATTAGCCCAAAAAGATCA
AGAGGAAATAATAAATCCAAATGATCCAATTATTCAAACACTGGACCAGCTTTTTAAATTTTGTTTACAACAAAACGCAT
CTGATATTCATATTGAACCTCAAAAAGCACATTTAATGATTCGATTAAGAATTGATGGCGTTTTACATATTTATAAAACA
TTGCCGCTTAATTTATCAAATCGGATAGTTTCACGGATTAAGCTTTTAGCTAAATTAGATATTAGCGAAACAAGATTTCC
ACAAGATGGGCAATTTCATTTTAAAACGTTATTAGGGGATGTTTTAGATTTTCGTGTATCAAGCCTGCCGACCAATTATG
GCGAAAAATTAGTACTACGTTTACAAAAAAATAAACCAACACAAGCTGATTTTTTATCTCTAGGATTTAATCAACATCAA
AAAGAAAAATTAATTTTTGCCTTAGAACAACCTCAAGGTTTAATTTTAGTCACAGGACCAACGGGGAGTGGTAAAAGTAT
TACGTTATATAGTGCACTCAGTTATTTAAATAACAATCAGAGACATATTATTACCGCTGAAGATCCGATTGAAATTGAAA
TCGAGGGAATTATTCAAACACAAGTAAATCGAGGTATTCAATTGGATTTTAGTCAATTATTACGAACTTTTTTACGCCAA
GATCCGGATATCATTATGTTTGGTGAAATTCGAGATGAAGAAAGTGCGCAAATGGCATTAAGAGCTGCGCAAACCGGGCA
TTTGGTACTTTCGACACTACATACTAATGACGCATTCTCTGCCGTGGAACGTTTATTACAATTAGGGATAAAAGAATATG
AATTGAATAATTCCTTGCTCTTAGTTATTGCGCAAAGATTAGTGCGAACGCTTTGCCCGAGCTGTTTAGGAAAAGGATGT
GAACATTGTTATCAGGGCTATAAAGGACGTACAGGCGTTTACCAATTGTTATCTAAAAATGGCAATTTTTTTGAAAAAAC
GACCGCTTGTTTAGACTTTAAAACACTTGCTGAAAGTGCAAAAGAAAAAGTCATGGCAAAAATAACAGATGAAACGGAAG
TGAAAAGAGTATTAGGCTATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FMQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

73.144

99.349

0.727
  pilB Haemophilus influenzae Rd KW20

56.608

98.482

0.557
  pilB Haemophilus influenzae 86-028NP

56.167

98.482

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

38.229

100

0.412
  pilB Vibrio cholerae strain A1552

37.8

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.293

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.41
  pilB Acinetobacter baumannii D1279779

37.827

100

0.408
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilF Thermus thermophilus HB27

35.776

100

0.36

Multiple sequence alignment