Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DDU33_RS09260 Genome accession   NZ_CP029206
Coordinates   1920040..1920486 (-) Length   148 a.a.
NCBI ID   WP_005823732.1    Uniprot ID   A0A2U8FKY8
Organism   Actinobacillus porcitonsillarum strain 9953L55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1915040..1925486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDU33_RS09235 (DDU33_09235) yacG 1915927..1916112 (-) 186 WP_108924833.1 DNA gyrase inhibitor YacG -
  DDU33_RS09240 (DDU33_09240) coaE 1916094..1916726 (-) 633 WP_108924834.1 dephospho-CoA kinase -
  DDU33_RS09245 (DDU33_09245) - 1916752..1917384 (-) 633 WP_244175317.1 prepilin peptidase -
  DDU33_RS09250 (DDU33_09250) pilC 1917416..1918612 (-) 1197 WP_108924836.1 type II secretion system F family protein Machinery gene
  DDU33_RS09255 (DDU33_09255) pilB 1918605..1919990 (-) 1386 WP_108924837.1 GspE/PulE family protein Machinery gene
  DDU33_RS09260 (DDU33_09260) pilA 1920040..1920486 (-) 447 WP_005823732.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DDU33_RS09265 (DDU33_09265) radA 1920672..1922051 (+) 1380 WP_108924838.1 DNA repair protein RadA -
  DDU33_RS09270 (DDU33_09270) - 1922086..1922571 (-) 486 WP_108924839.1 dihydrofolate reductase -
  DDU33_RS09275 (DDU33_09275) proB 1922694..1923785 (+) 1092 WP_108924840.1 glutamate 5-kinase -
  DDU33_RS09280 (DDU33_09280) - 1923809..1924573 (+) 765 WP_108924841.1 TSUP family transporter -
  DDU33_RS09285 (DDU33_09285) nth 1924574..1925209 (+) 636 WP_005818971.1 endonuclease III -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15630.80 Da        Isoelectric Point: 8.4638

>NTDB_id=291147 DDU33_RS09260 WP_005823732.1 1920040..1920486(-) (pilA) [Actinobacillus porcitonsillarum strain 9953L55]
MKSQFLFKTAKAFTLIELMIVIAIIAILATIAIPSYTTYTQKAALSELLRASSSYKSDVEVCIYNTGAVTNCSGGSNGIQ
ANKTSSDETKYLKSVSVTNGVISVAGKGNVDGFGYSMTPTFANNTISWKTACTGSDTSLFPANFCSTN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=291147 DDU33_RS09260 WP_005823732.1 1920040..1920486(-) (pilA) [Actinobacillus porcitonsillarum strain 9953L55]
ATGAAATCTCAATTTTTATTTAAAACCGCAAAGGCATTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCCAT
TCTTGCCACGATTGCGATCCCTTCATATACCACCTATACCCAGAAAGCGGCACTATCTGAATTACTCAGAGCATCTTCCT
CTTATAAATCAGATGTGGAGGTGTGTATCTACAATACCGGTGCTGTGACGAATTGTTCGGGGGGATCAAATGGTATTCAA
GCCAATAAAACCTCAAGTGATGAAACCAAATATTTGAAATCTGTTTCGGTTACGAATGGGGTAATTAGTGTGGCAGGAAA
AGGAAATGTTGATGGCTTTGGTTATAGCATGACCCCAACATTTGCAAATAATACGATTAGTTGGAAAACCGCATGTACGG
GTTCAGATACCAGCTTATTCCCTGCAAATTTCTGTTCGACTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FKY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

69.863

98.649

0.689
  pilA Haemophilus influenzae 86-028NP

58.824

91.892

0.541
  pilA Haemophilus influenzae Rd KW20

54.422

99.324

0.541
  comP Acinetobacter baylyi ADP1

41.135

95.27

0.392

Multiple sequence alignment