Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DDU33_RS09250 Genome accession   NZ_CP029206
Coordinates   1917416..1918612 (-) Length   398 a.a.
NCBI ID   WP_108924836.1    Uniprot ID   A0A2U8FL50
Organism   Actinobacillus porcitonsillarum strain 9953L55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1912416..1923612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DDU33_RS09215 (DDU33_09215) citD 1912505..1912792 (-) 288 WP_005820944.1 citrate lyase acyl carrier protein -
  DDU33_RS09220 (DDU33_09220) metF 1913055..1913933 (+) 879 WP_005820943.1 methylenetetrahydrofolate reductase -
  DDU33_RS09225 (DDU33_09225) - 1914046..1914675 (-) 630 WP_108924831.1 hypothetical protein -
  DDU33_RS09230 (DDU33_09230) - 1914856..1915818 (-) 963 WP_108924832.1 helix-turn-helix transcriptional regulator -
  DDU33_RS09235 (DDU33_09235) yacG 1915927..1916112 (-) 186 WP_108924833.1 DNA gyrase inhibitor YacG -
  DDU33_RS09240 (DDU33_09240) coaE 1916094..1916726 (-) 633 WP_108924834.1 dephospho-CoA kinase -
  DDU33_RS09245 (DDU33_09245) - 1916752..1917384 (-) 633 WP_244175317.1 prepilin peptidase -
  DDU33_RS09250 (DDU33_09250) pilC 1917416..1918612 (-) 1197 WP_108924836.1 type II secretion system F family protein Machinery gene
  DDU33_RS09255 (DDU33_09255) pilB 1918605..1919990 (-) 1386 WP_108924837.1 GspE/PulE family protein Machinery gene
  DDU33_RS09260 (DDU33_09260) pilA 1920040..1920486 (-) 447 WP_005823732.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DDU33_RS09265 (DDU33_09265) radA 1920672..1922051 (+) 1380 WP_108924838.1 DNA repair protein RadA -
  DDU33_RS09270 (DDU33_09270) - 1922086..1922571 (-) 486 WP_108924839.1 dihydrofolate reductase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 46043.68 Da        Isoelectric Point: 9.4614

>NTDB_id=291145 DDU33_RS09250 WP_108924836.1 1917416..1918612(-) (pilC) [Actinobacillus porcitonsillarum strain 9953L55]
MFKVYEYQWSAINRFEQKQKGKRLAKNQEELENLLLKKGYHHIKISRNFALAKSPQSEEITQVLSQLAMLVKAKIPLKSA
LAMILENCTQIKIYLWLEALIKWIELGYAFSTALEKSEKYIPQQEIQLIKMGEISGKLETILQNIAKARKEKETTLKKVK
KILFYPIVVLTISFSLSIGLLLFIVPQFAELYGAKEKSLPMITEILFSLSAFLQEYISEFIFFFIVIMLLFRFIAKKTAW
FLHVKNRLLKATPVFNQIFNYARIVFFHQNLALMLNANIRLDAILRSFLSETPDDPQLQQEITTALQLLKQGYSLSESLN
PNLFNEQVIQMLFIGEKSGNLAQMSEHIHEIYQQKLDYQIDIISQLLEPMLMLIMGIIVGTIIVGLYLPIFDMGSLVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=291145 DDU33_RS09250 WP_108924836.1 1917416..1918612(-) (pilC) [Actinobacillus porcitonsillarum strain 9953L55]
ATGTTTAAGGTATATGAATATCAATGGTCGGCAATAAATCGATTTGAGCAAAAACAAAAAGGGAAGCGATTAGCAAAAAA
TCAAGAAGAGTTAGAAAATTTGCTGTTAAAAAAGGGTTATCATCATATCAAAATAAGTCGGAATTTTGCTTTAGCAAAAT
CGCCACAATCAGAAGAAATAACTCAAGTCCTTTCTCAATTAGCAATGCTGGTAAAAGCTAAAATTCCTTTAAAATCGGCA
TTGGCGATGATTTTAGAAAATTGTACACAAATAAAAATTTATTTATGGCTAGAAGCCTTAATCAAATGGATTGAATTAGG
CTATGCATTTTCAACGGCATTAGAAAAATCTGAAAAATATATCCCACAACAAGAAATCCAACTGATTAAAATGGGAGAGA
TAAGTGGTAAGTTAGAAACTATTTTACAAAATATAGCTAAAGCCAGAAAAGAGAAAGAAACAACCTTAAAAAAGGTAAAA
AAGATCTTATTTTACCCCATTGTTGTTTTAACGATCTCTTTTTCACTTTCAATTGGTTTATTGTTATTTATCGTTCCGCA
ATTTGCAGAATTATATGGCGCAAAAGAGAAAAGCCTTCCGATGATTACGGAAATCTTATTTAGTCTATCGGCATTTTTAC
AAGAATATATTAGCGAATTTATTTTCTTTTTTATTGTTATCATGTTGCTTTTCAGATTTATAGCTAAAAAAACAGCTTGG
TTTTTACATGTTAAAAATCGTTTATTGAAAGCCACACCTGTTTTTAATCAGATTTTTAATTATGCGAGAATTGTCTTTTT
TCATCAAAATTTAGCCCTAATGTTGAATGCGAATATCAGGCTGGATGCAATATTACGCTCTTTTTTATCTGAAACACCTG
ATGATCCACAATTACAGCAAGAAATTACAACAGCCCTTCAACTATTAAAGCAAGGTTATTCACTATCGGAAAGTTTAAAT
CCAAATTTGTTTAATGAGCAGGTCATTCAAATGCTGTTTATTGGCGAAAAAAGTGGCAATTTAGCACAAATGAGCGAGCA
TATTCATGAAATTTATCAGCAAAAATTGGATTATCAAATTGACATTATTTCTCAATTATTAGAGCCGATGTTAATGTTGA
TTATGGGGATTATTGTAGGGACGATTATTGTGGGCTTATACCTTCCTATTTTTGATATGGGATCGTTAGTAGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(269-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FL50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

55.276

100

0.553
  pilC Haemophilus influenzae Rd KW20

43.951

100

0.447
  pilC Haemophilus influenzae 86-028NP

43.457

100

0.442

Multiple sequence alignment