Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DB847_RS02305 Genome accession   NZ_CP028897
Coordinates   441912..443618 (-) Length   568 a.a.
NCBI ID   WP_108649263.1    Uniprot ID   -
Organism   Dongshaea marina strain DM2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436912..448618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB847_RS02275 mutT 437561..437959 (+) 399 WP_108652886.1 8-oxo-dGTP diphosphatase MutT -
  DB847_RS02280 yacG 438057..438245 (-) 189 WP_407644431.1 DNA gyrase inhibitor YacG -
  DB847_RS02285 zapD 438257..438987 (-) 731 Protein_424 cell division protein ZapD -
  DB847_RS02290 coaE 439015..439623 (-) 609 WP_234418492.1 dephospho-CoA kinase -
  DB847_RS02295 pilD 439626..440513 (-) 888 WP_108649261.1 A24 family peptidase Machinery gene
  DB847_RS02300 pilC 440647..441894 (-) 1248 WP_108649262.1 type II secretion system F family protein Machinery gene
  DB847_RS02305 pilB 441912..443618 (-) 1707 WP_108649263.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DB847_RS02310 - 443624..444064 (-) 441 WP_108649264.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DB847_RS02315 - 444701..445585 (+) 885 WP_108649240.1 IS982 family transposase -
  DB847_RS02320 nadC 445624..446463 (-) 840 WP_108649265.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DB847_RS02325 ampD 446588..447145 (+) 558 WP_108649266.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DB847_RS02330 - 447323..448105 (+) 783 WP_108649267.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63060.25 Da        Isoelectric Point: 4.8379

>NTDB_id=288324 DB847_RS02305 WP_108649263.1 441912..443618(-) (pilB) [Dongshaea marina strain DM2]
MNASTSSGLAISLAASSLITEDNAKRYCLEAKHQHKPLVSYLVDSEIIESEQLAQFCEQEYGIPLLDLEAFNLDEIPQKF
INEKLIEKYHALPIYTQGHTLYIAMSDPTNVTALEDFGFSFGLHTEALLVDELKLQKAISKVLESDMDSLDMDDLDNDEI
DGMEVADEESRLDEVSAPDDDDAPIVRYINKLLMDAIRKDASDLHFEPYEHKYRIRFRIDGILHEVANPPINLANRFSAR
LKVMSRLDIAERRLPQDGRIKLKLSRNKAIDLRVNTLPTLWGEKVVLRILDSSAAKLNIDILGFDENQKKLYTECLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLPGINQVHIHPKAGLTFASALRAFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMLNMGVPAYNIASSVTLIMAQRLARRLCDHCKEPEEVPEVQLLELGFS
EEQVKKGFTVYKPKGCPECSGGYKGRVGLYEMLVMSENIAKLIMEGANSLDIAELGIKEGMQSLHMSGLVKVTQGVTSLA
EVNRVTSR

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=288324 DB847_RS02305 WP_108649263.1 441912..443618(-) (pilB) [Dongshaea marina strain DM2]
ATGAACGCAAGCACTTCGAGCGGCCTGGCGATCAGCCTGGCCGCTTCTTCTCTCATCACAGAGGATAATGCCAAGCGCTA
CTGCCTGGAGGCTAAGCATCAGCATAAGCCCCTGGTTTCCTACCTGGTCGATAGCGAGATTATCGAAAGCGAGCAGCTGG
CCCAGTTCTGCGAGCAGGAGTATGGGATCCCACTGCTGGATCTCGAGGCCTTTAACTTAGATGAGATCCCTCAGAAATTT
ATCAACGAGAAATTGATTGAGAAATACCATGCATTGCCGATCTACACCCAGGGCCATACCCTGTATATCGCAATGTCCGA
TCCCACCAATGTCACCGCCCTCGAGGATTTTGGATTTAGCTTTGGCCTGCACACAGAAGCGCTGCTGGTCGATGAGCTCA
AGCTGCAAAAAGCCATATCCAAAGTGCTTGAGAGCGACATGGACTCCCTGGACATGGACGATCTAGACAATGACGAGATC
GATGGCATGGAGGTCGCCGACGAAGAGAGCCGCCTCGATGAGGTCTCGGCTCCCGACGATGATGACGCGCCGATCGTCCG
TTACATCAACAAGCTGTTGATGGATGCAATTCGCAAGGACGCCTCGGATCTGCACTTTGAGCCCTATGAGCACAAGTACC
GGATCCGTTTTCGTATCGATGGGATTTTGCATGAGGTGGCCAATCCGCCGATCAACCTGGCCAATCGCTTCTCGGCCCGC
CTCAAGGTCATGTCCCGCCTGGATATCGCCGAGCGCCGCCTGCCCCAGGATGGCCGAATCAAGCTCAAGCTGTCGCGCAA
CAAGGCGATCGATCTGCGGGTCAACACACTGCCCACCCTATGGGGTGAAAAGGTGGTGCTGCGTATCCTCGATTCCTCCG
CCGCCAAGCTGAATATCGATATCCTGGGTTTTGATGAAAACCAGAAAAAGCTCTATACCGAGTGCCTGGCCAAGCCCCAG
GGGATGATCCTGGTCACAGGCCCCACCGGAAGCGGTAAGACGGTATCTTTGTACACCGGACTGAATATTCTTAACACAGC
CGAACGCAACATCTCCACCGCTGAGGATCCGGTGGAGATCAACTTGCCCGGGATCAACCAGGTGCATATCCATCCCAAGG
CAGGACTGACCTTTGCCAGCGCCCTGCGCGCCTTTTTGCGCCAGGATCCCGATATCGTGATGGTGGGAGAGATCCGGGAC
CTGGAAACCGCCGAGATCGCGATCAAAGCGGCGCAAACCGGTCACCTGGTGCTCTCGACCCTACACACCAATTCAGCTGC
AGAGACTCTGACCCGGATGCTCAATATGGGAGTCCCGGCTTACAACATCGCCTCATCGGTCACCCTCATCATGGCTCAGC
GCCTGGCTCGACGCCTGTGCGATCACTGCAAAGAGCCCGAAGAGGTCCCCGAAGTACAGCTACTGGAGCTGGGTTTTTCC
GAGGAGCAAGTGAAAAAAGGCTTTACGGTTTATAAGCCCAAGGGGTGCCCCGAGTGCTCCGGAGGCTACAAAGGACGAGT
CGGCCTCTATGAGATGTTGGTGATGTCGGAAAATATTGCCAAACTTATCATGGAGGGGGCCAACTCCCTGGATATCGCTG
AGCTTGGGATCAAAGAGGGGATGCAGTCCCTGCATATGTCAGGTCTGGTCAAGGTCACCCAGGGAGTCACCAGCCTGGCC
GAAGTCAACCGGGTCACCAGCCGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.595

99.12

0.551
  pilB Acinetobacter baumannii D1279779

57.671

95.246

0.549
  pilB Legionella pneumophila strain ERS1305867

55.91

93.838

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilB Vibrio cholerae strain A1552

53.875

93.134

0.502
  pilB Vibrio campbellii strain DS40M4

52.372

92.782

0.486
  pilF Neisseria gonorrhoeae MS11

47.788

99.472

0.475
  pilF Thermus thermophilus HB27

40.643

93.134

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

93.134

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.798

96.655

0.375

Multiple sequence alignment