Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Sa5Y_RS02445 Genome accession   NZ_CP028892
Coordinates   486155..487381 (-) Length   408 a.a.
NCBI ID   WP_000648506.1    Uniprot ID   A0A0E3W4T9
Organism   Vibrio cholerae strain Sa5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481155..492381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Sa5Y_RS02415 (Sa5Y_VC00476) parC 481156..483441 (+) 2286 WP_046121363.1 DNA topoisomerase IV subunit A -
  Sa5Y_RS02420 (Sa5Y_VC00477) yacG 483490..483687 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  Sa5Y_RS02430 (Sa5Y_VC00478) zapD 483845..484585 (-) 741 WP_000207193.1 cell division protein ZapD -
  Sa5Y_RS02435 (Sa5Y_VC00479) coaE 484617..485225 (-) 609 WP_162298799.1 dephospho-CoA kinase -
  Sa5Y_RS02440 (Sa5Y_VC00480) pilD 485222..486097 (-) 876 WP_033932898.1 A24 family peptidase Machinery gene
  Sa5Y_RS02445 (Sa5Y_VC00481) pilC 486155..487381 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  Sa5Y_RS02450 (Sa5Y_VC00482) pilB 487424..489112 (-) 1689 WP_108243691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Sa5Y_RS02455 (Sa5Y_VC00483) pilA 489116..489544 (-) 429 WP_094393042.1 pilin Machinery gene
  Sa5Y_RS02460 (Sa5Y_VC00484) nadC 489789..490679 (-) 891 WP_094393041.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Sa5Y_RS02470 (Sa5Y_VC00485) ampD 490981..491526 (+) 546 WP_094393039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Sa5Y_RS02475 (Sa5Y_VC00486) fldB 491675..492193 (-) 519 WP_069503252.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45090.38 Da        Isoelectric Point: 10.3424

>NTDB_id=288208 Sa5Y_RS02445 WP_000648506.1 486155..487381(-) (pilC) [Vibrio cholerae strain Sa5Y]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=288208 Sa5Y_RS02445 WP_000648506.1 486155..487381(-) (pilC) [Vibrio cholerae strain Sa5Y]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGTCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACTCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATTACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTTGTGTTGGTCGCACTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAGTTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGCAA
TGTGCTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACGCTTGCCACCAGCTTTGCCGCGGGGATTCCGATCCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGACGCTTTTCCGGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCAGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACAGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTGTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3W4T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

74

98.039

0.725
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment