Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Sa5Y_RS02455 Genome accession   NZ_CP028892
Coordinates   489116..489544 (-) Length   142 a.a.
NCBI ID   WP_094393042.1    Uniprot ID   -
Organism   Vibrio cholerae strain Sa5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484116..494544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Sa5Y_RS02435 (Sa5Y_VC00479) coaE 484617..485225 (-) 609 WP_162298799.1 dephospho-CoA kinase -
  Sa5Y_RS02440 (Sa5Y_VC00480) pilD 485222..486097 (-) 876 WP_033932898.1 A24 family peptidase Machinery gene
  Sa5Y_RS02445 (Sa5Y_VC00481) pilC 486155..487381 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  Sa5Y_RS02450 (Sa5Y_VC00482) pilB 487424..489112 (-) 1689 WP_108243691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Sa5Y_RS02455 (Sa5Y_VC00483) pilA 489116..489544 (-) 429 WP_094393042.1 pilin Machinery gene
  Sa5Y_RS02460 (Sa5Y_VC00484) nadC 489789..490679 (-) 891 WP_094393041.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Sa5Y_RS02470 (Sa5Y_VC00485) ampD 490981..491526 (+) 546 WP_094393039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Sa5Y_RS02475 (Sa5Y_VC00486) fldB 491675..492193 (-) 519 WP_069503252.1 flavodoxin FldB -
  Sa5Y_RS02480 (Sa5Y_VC00487) xerD 492331..493239 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  Sa5Y_RS02485 (Sa5Y_VC00488) dsbC 493256..494008 (+) 753 WP_059260674.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14792.96 Da        Isoelectric Point: 8.4616

>NTDB_id=288210 Sa5Y_RS02455 WP_094393042.1 489116..489544(-) (pilA) [Vibrio cholerae strain Sa5Y]
MKAYKNKLQKGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLENGSFPAIEDLAPPSAS
IGTIQYGEAQASGAGSIVFAFGTSGASPSVVNRNVTLARDENGSWKCTTTIDTKIAPKGCTP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=288210 Sa5Y_RS02455 WP_094393042.1 489116..489544(-) (pilA) [Vibrio cholerae strain Sa5Y]
ATGAAAGCGTATAAAAACAAACTACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGTGTGTT
GGCTGCCATTGCAGTGCCTCAGTACCAAAAATACGTCGCAAAAAGTGAGGCAGCAGCAGCACTGGCTTCGATCACTGGAC
ATAGAACAAACGTTGAAACATACGTATTGGAAAATGGTTCCTTCCCAGCAATAGAAGATTTAGCCCCGCCATCAGCAAGT
ATAGGCACTATTCAATATGGTGAGGCACAAGCATCTGGAGCTGGCAGCATAGTTTTTGCCTTCGGTACAAGTGGAGCAAG
CCCTAGTGTTGTAAATAGAAATGTTACTTTAGCTCGTGATGAAAATGGATCGTGGAAATGTACAACCACTATTGATACAA
AAATCGCGCCTAAAGGCTGTACACCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.656

100

0.549
  pilA Vibrio cholerae strain A1552

51.656

100

0.549
  pilA Vibrio cholerae C6706

51.656

100

0.549
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  pilA Vibrio parahaemolyticus RIMD 2210633

41.667

100

0.423
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.972

100

0.415
  pilA Acinetobacter baumannii strain A118

41.606

96.479

0.401
  comP Acinetobacter baylyi ADP1

38.356

100

0.394
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

99.296

0.394
  pilE Neisseria gonorrhoeae strain FA1090

33.121

100

0.366

Multiple sequence alignment