Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DB248_RS00660 Genome accession   NZ_CP028841
Coordinates   101881..102819 (+) Length   312 a.a.
NCBI ID   WP_010921798.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain CCUG 4207     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96881..107819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB248_RS00650 (DB248_00650) rpoC 97630..101271 (+) 3642 WP_010921797.1 DNA-directed RNA polymerase subunit beta' -
  DB248_RS00655 (DB248_00655) - 101423..101788 (+) 366 WP_002986560.1 DUF1033 family protein -
  DB248_RS00660 (DB248_00660) comYA 101881..102819 (+) 939 WP_010921798.1 competence type IV pilus ATPase ComGA Machinery gene
  DB248_RS00665 (DB248_00665) comYB 102755..103789 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DB248_RS00670 (DB248_00670) comYC 103791..104117 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DB248_RS00675 (DB248_00675) comGD 104092..104520 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DB248_RS00680 (DB248_00680) comGE 104477..104761 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DB248_RS00685 (DB248_00685) comYF 104754..105188 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  DB248_RS00690 (DB248_00690) comGG 105172..105498 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DB248_RS00695 (DB248_00695) comYH 105596..106549 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  DB248_RS00700 (DB248_00700) - 106608..107804 (+) 1197 WP_029714175.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35068.31 Da        Isoelectric Point: 8.5825

>NTDB_id=287999 DB248_RS00660 WP_010921798.1 101881..102819(+) (comYA) [Streptococcus pyogenes strain CCUG 4207]
MVQALAKAILAKAEQVHAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=287999 DB248_RS00660 WP_010921798.1 101881..102819(+) (comYA) [Streptococcus pyogenes strain CCUG 4207]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCATGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment