Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDG57_RS16315 Genome accession   NZ_CP028800
Coordinates   3346480..3347655 (+) Length   391 a.a.
NCBI ID   WP_087540366.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCHAJ59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3341480..3352655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG57_RS16290 (CDG57_16275) - 3342144..3342617 (-) 474 WP_004914520.1 hypothetical protein -
  CDG57_RS16295 (CDG57_16280) guaD 3342803..3344128 (+) 1326 WP_087540365.1 guanine deaminase -
  CDG57_RS16300 (CDG57_16285) hpt 3344345..3344872 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  CDG57_RS16305 (CDG57_16290) - 3345101..3345352 (+) 252 WP_004914533.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  CDG57_RS16310 (CDG57_16295) - 3345402..3346100 (-) 699 WP_005403992.1 DsbC family protein -
  CDG57_RS16315 (CDG57_16300) pilU 3346480..3347655 (+) 1176 WP_087540366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG57_RS16320 (CDG57_16305) mnmE 3347711..3349066 (-) 1356 WP_087540377.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  CDG57_RS16325 (CDG57_16310) yidC 3349157..3350914 (-) 1758 WP_087540368.1 membrane protein insertase YidC -
  CDG57_RS16330 (CDG57_16315) yidD 3350920..3351240 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  CDG57_RS16335 (CDG57_16320) rnpA 3351250..3351645 (-) 396 WP_004950005.1 ribonuclease P protein component -
  CDG57_RS16340 (CDG57_16325) rpmH 3351674..3351808 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44293.85 Da        Isoelectric Point: 6.4451

>NTDB_id=287826 CDG57_RS16315 WP_087540366.1 3346480..3347655(+) (pilU) [Acinetobacter junii strain WCHAJ59]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFVNDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=287826 CDG57_RS16315 WP_087540366.1 3346480..3347655(+) (pilU) [Acinetobacter junii strain WCHAJ59]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGTTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CTGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGTAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCAAGGTTTCGGGTCAATGTTTTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGTCAAGGTCGTCGAGCTGCCATTGAAA
TCCTCCTCAATACGCCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCGGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAGGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

51.374

93.095

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

85.678

0.368
  pilT Acinetobacter nosocomialis M2

43.865

83.376

0.366
  pilT Acinetobacter baumannii D1279779

43.865

83.376

0.366
  pilT Acinetobacter baumannii strain A118

43.865

83.376

0.366
  pilT Acinetobacter baylyi ADP1

42.262

85.934

0.363

Multiple sequence alignment