Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG57_RS14445 Genome accession   NZ_CP028800
Coordinates   2945804..2947528 (+) Length   574 a.a.
NCBI ID   WP_087539739.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCHAJ59     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2940804..2952528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG57_RS14410 (CDG57_14400) nusA 2941656..2943140 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  CDG57_RS14415 (CDG57_14405) rimP 2943178..2943702 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  CDG57_RS14435 (CDG57_14425) secG 2944325..2944654 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  CDG57_RS14440 (CDG57_14430) tpiA 2944667..2945458 (-) 792 WP_005403576.1 triose-phosphate isomerase -
  CDG57_RS14445 (CDG57_14435) pilB 2945804..2947528 (+) 1725 WP_087539739.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG57_RS14450 (CDG57_14440) pilC 2947553..2948779 (+) 1227 WP_087539738.1 type II secretion system F family protein Machinery gene
  CDG57_RS14455 (CDG57_14445) pilD 2948779..2949639 (+) 861 WP_087539737.1 prepilin peptidase Machinery gene
  CDG57_RS14460 (CDG57_14450) coaE 2949641..2950240 (+) 600 WP_087539736.1 dephospho-CoA kinase -
  CDG57_RS14465 (CDG57_14455) - 2950237..2951148 (-) 912 WP_004958607.1 DMT family transporter -
  CDG57_RS14470 (CDG57_14460) rlmB 2951179..2951928 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG57_RS14475 (CDG57_14465) - 2952120..2952446 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63654.81 Da        Isoelectric Point: 5.1367

>NTDB_id=287814 CDG57_RS14445 WP_087539739.1 2945804..2947528(+) (pilB) [Acinetobacter junii strain WCHAJ59]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIEQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LEKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSLL
EMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQ
GITSLQEVNRVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=287814 CDG57_RS14445 WP_087539739.1 2945804..2947528(+) (pilB) [Acinetobacter junii strain WCHAJ59]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTTATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAACACAATAAACTCGAAAAACTAATTGAACAGCAGTTTGGAGATAGTGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCCCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGCGCCTCTGACTTACATTTTGAACCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGAGTACTCAGACAAATTGCTAATCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGAAAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTAGAAACAGCTGAAATTGCTATCAAAGCAGCCCAAACAGGTCACATGGTGATGTCGACTTTGCA
CACCAATAGCGCTCCAGAAACACTCACACGTTTACGTAATATGGGAGTTCAGTCTTTCAATATTGCGACTTCGGTTAATC
TAGTAATTGCTCAACGTTTAGCTCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTTCTA
GAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATGGGA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.729

100

0.81
  pilB Acinetobacter baylyi ADP1

76.348

100

0.765
  pilB Legionella pneumophila strain ERS1305867

52.602

93.728

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

97.038

0.483
  pilF Neisseria gonorrhoeae MS11

50.091

95.296

0.477
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

50.87

90.07

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.416

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

92.857

0.361
  pilF Thermus thermophilus HB27

36.637

98.432

0.361

Multiple sequence alignment