Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CDG57_RS14455 Genome accession   NZ_CP028800
Coordinates   2948779..2949639 (+) Length   286 a.a.
NCBI ID   WP_087539737.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCHAJ59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2943779..2954639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG57_RS14435 (CDG57_14425) secG 2944325..2944654 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  CDG57_RS14440 (CDG57_14430) tpiA 2944667..2945458 (-) 792 WP_005403576.1 triose-phosphate isomerase -
  CDG57_RS14445 (CDG57_14435) pilB 2945804..2947528 (+) 1725 WP_087539739.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG57_RS14450 (CDG57_14440) pilC 2947553..2948779 (+) 1227 WP_087539738.1 type II secretion system F family protein Machinery gene
  CDG57_RS14455 (CDG57_14445) pilD 2948779..2949639 (+) 861 WP_087539737.1 prepilin peptidase Machinery gene
  CDG57_RS14460 (CDG57_14450) coaE 2949641..2950240 (+) 600 WP_087539736.1 dephospho-CoA kinase -
  CDG57_RS14465 (CDG57_14455) - 2950237..2951148 (-) 912 WP_004958607.1 DMT family transporter -
  CDG57_RS14470 (CDG57_14460) rlmB 2951179..2951928 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG57_RS14475 (CDG57_14465) - 2952120..2952446 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32053.65 Da        Isoelectric Point: 7.8116

>NTDB_id=287816 CDG57_RS14455 WP_087539737.1 2948779..2949639(+) (pilD) [Acinetobacter junii strain WCHAJ59]
MQSFFFYFLENPTALYLAVGLISLCIGSFLNVVIFRTPKMMEQEWHQECQMLLHPEQPIIDQSKLTLSQPVSTCPKCQTP
IKWFQNIPLISWLALRGKCGTCKNPISIRYPLIELLTMICSLIVVAVFGATIQMLFGLILTWTLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFAIYTTANAAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSM
VGAIIGIMLMKMRGENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=287816 CDG57_RS14455 WP_087539737.1 2948779..2949639(+) (pilD) [Acinetobacter junii strain WCHAJ59]
ATGCAATCTTTTTTCTTTTACTTCCTTGAAAATCCAACTGCACTTTACCTTGCAGTTGGATTAATCAGTCTGTGCATTGG
CAGTTTTTTAAATGTTGTGATCTTTCGCACTCCAAAAATGATGGAGCAAGAATGGCATCAAGAATGCCAAATGCTTTTGC
ACCCTGAACAGCCGATCATAGATCAGAGCAAGTTAACATTAAGTCAGCCAGTTTCTACCTGCCCTAAGTGTCAAACGCCA
ATTAAATGGTTCCAAAATATTCCTCTTATTAGTTGGTTAGCACTCCGTGGTAAATGTGGCACTTGCAAGAATCCAATTAG
CATTCGCTACCCGTTGATTGAATTACTTACTATGATCTGTTCATTGATCGTTGTGGCAGTATTCGGTGCAACAATACAGA
TGCTTTTCGGCTTAATTCTAACGTGGACACTTATTGCACTTACTTTTATTGATTTTGATACACAATTACTACCTGATCGC
TTTACGCTACCTTTGGCAGCATTAGGTCTGGGTATTAATACTTTTGCAATCTATACAACGGCTAACGCTGCGATCTGGGG
CTATTTAATCGGTTTTCTATGTCTTTGGATTGTATATTACCTATTTAAAGTCATCACCGGTAAAGAAGGCATGGGGTATG
GTGATTTTAAATTGCTTGCAGCACTTGGTGCATGGATGGGTCCTATGATGTTACCCCTCATCGTATTACTCTCTTCTATG
GTAGGTGCAATTATTGGTATTATGCTGATGAAAATGCGCGGTGAAAATCAACCTTTTGCATTTGGTCCTTATATTGCAAT
TGCCGGTTGGATTGCCTTTCTATGGGGTGAGCAAATTATGAAAGTGTATCTTGGGGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

82.168

100

0.822
  pilD Acinetobacter baumannii D1279779

81.818

100

0.818
  pilD Vibrio cholerae strain A1552

52.778

100

0.531
  pilD Vibrio campbellii strain DS40M4

51.128

93.007

0.476
  pilD Neisseria gonorrhoeae MS11

45.788

95.455

0.437

Multiple sequence alignment