Detailed information    

experimental Experimentally validated

Overview

Name   uvrC   Type   Machinery gene
Locus tag   SP_RS03035 Genome accession   NC_003028
Coordinates   585747..587591 (+) Length   614 a.a.
NCBI ID   WP_001061130.1    Uniprot ID   Q97S07
Organism   Streptococcus pneumoniae TIGR4     
Function   homologous recombination   
Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.


Genomic Context

Location: 580747..592591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS03015 (SP_0614) - 581579..582358 (+) 780 WP_000290025.1 alpha/beta hydrolase -
  SP_RS03020 (SP_0615) murM 582470..583690 (+) 1221 WP_001808632.1 peptidoglycan bridge formation alanyltransferase MurM -
  SP_RS03025 (SP_0616) murN 583695..584927 (+) 1233 WP_001229501.1 peptidoglycan bridge formation alanyltransferase MurN -
  SP_RS03030 (SP_0617) - 585006..585707 (+) 702 WP_001267410.1 M57 family metalloprotease -
  SP_RS03035 (SP_0618) uvrC 585747..587591 (+) 1845 WP_001061130.1 excinuclease ABC subunit UvrC Machinery gene
  SP_RS03040 (SP_0619) - 587581..588414 (+) 834 WP_001015785.1 metallophosphoesterase family protein -
  SP_RS03045 (SP_0620) - 588596..589396 (-) 801 WP_000714614.1 amino acid ABC transporter substrate-binding protein -
  SP_RS03050 (SP_0622) - 589567..590172 (+) 606 WP_000670146.1 nitroreductase family protein -
  SP_RS03055 (SP_0623) pepV 590185..591585 (+) 1401 WP_000125043.1 dipeptidase PepV -
  SP_RS03060 (SP_0624) - 591639..592217 (+) 579 WP_000078155.1 uracil-DNA glycosylase family protein -

Sequence

Protein


Download         Length: 614 a.a.        Molecular weight: 70408.95 Da        Isoelectric Point: 9.1161

>NTDB_id=693 SP_RS03035 WP_001061130.1 585747..587591(+) (uvrC) [Streptococcus pneumoniae TIGR4]
MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSYFRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEIN
LIKENKPKYNIMLKDDKSYPFIKITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERAAEYRDLIQAIGTLRTKQRVMAK
DLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNLFPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKAL
VDSKILKPQRGEKKQLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGTSPVSAMVV
FVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLIVIDGGQGQVNIAKQVIQEELGLDIPIAGLQ
KNDKHQTHELLFGDPLEVVDLSRNSQEFFLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSL
TKIKEASVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP

Nucleotide


Download         Length: 1845 bp        

>NTDB_id=693 SP_RS03035 WP_001061130.1 585747..587591(+) (uvrC) [Streptococcus pneumoniae TIGR4]
ATGAACAACTTGATTAAATCAAAACTAGAGCTCTTGCCGACCAGCCCTGGTTGCTACATTCATAAGGATAAAAATGGCAC
CATTATCTATGTAGGAAAGGCTAAAAATCTGCGTAATCGAGTACGGTCCTATTTTCGTGGAAGTCATGATACCAAGACAG
AGGCTCTGGTGTCTGAAATTGTGGATTTTGAATTTATTGTTACGGAGTCTAATATTGAGGCACTTCTCCTAGAAATCAAC
CTGATCAAGGAAAACAAGCCCAAGTACAATATCATGCTCAAGGATGACAAGTCCTATCCTTTCATCAAAATCACCAATGA
GCGCTATCCACGCTTGATTATCACTCGTCAGGTCAAAAAGGACGGAGGTCTTTATTTTGGACCCTATCCCGATGTGGGGG
CAGCCAATGAAATCAAGCGGTTGCTGGATCGGATATTCCCTTTTCGTAAGTGTACCAACCCGCCCTCTAAGGTCTGTTTT
TATTACCATATCGGCCAGTGTATGGCCCACACCATCTGTAAGAAGGATGAGGCTTATTTCAAGTCTATGGCCCAGGAGGT
GTCTGATTTTCTGAAAGGTCAGGATGACAAAATCATCGATGATCTCAAGAGTAAAATGGCAGTAGCAGCACAAAGTATGG
AGTTTGAACGTGCGGCGGAATACCGTGACCTGATTCAGGCTATTGGAACGCTTCGAACCAAGCAACGGGTCATGGCGAAA
GATTTGCAAAATCGCGATGTCTTTGGCTACTATGTGGATAAGGGCTGGATGTGTGTGCAGGTTTTCTTTGTCCGTCAGGG
TAAGCTCATCGAGCGCGATGTCAATCTCTTCCCCTACTTCAATGATCCAGATGAGGATTTTTTGACCTATGTAGGACAAT
TCTATCAAGAAAAATCTCATCTAGTTCCCAATGAGGTACTGATTCCGCAGGATATTGACGAAGAAGCTGTCAAGGCTTTG
GTGGATTCCAAGATTCTTAAGCCTCAACGTGGAGAGAAAAAACAACTGGTCAATCTAGCCATAAAAAATGCTCGTGTTAG
TCTAGAGCAGAAGTTCAATCTGCTAGAAAAATCTGTCGAAAAGACTCAAGGAGCTATTGAAAATCTAGGGCGTTTGCTCC
AAATCCCGACCCCAGTACGTATCGAGTCCTTCGATAACTCTAATATCATGGGAACTAGCCCTGTTTCGGCTATGGTGGTC
TTTGTCAACGGTAAACCGAGTAAGAAGGATTACCGTAAGTACAAGATAAAAACGGTTGTTGGACCAGACGACTATGCCAG
CATGAGAGAGGTCATTCGCAGACGCTATGGTCGAGTACAGCGTGAGGCTTTGACTCCTCCAGATTTGATTGTGATTGATG
GGGGGCAAGGTCAAGTCAATATCGCTAAGCAGGTTATCCAAGAGGAACTGGGCTTGGATATTCCAATTGCTGGGCTGCAA
AAGAATGATAAGCACCAAACCCATGAATTGCTCTTTGGAGATCCGCTTGAGGTGGTGGATTTGTCTCGCAATTCTCAGGA
ATTTTTCCTCCTCCAACGCATCCAAGATGAGGTGCACCGCTTTGCTATCACTTTCCACCGCCAACTGCGCTCCAAAAATT
CTTTCTCATCTCAATTGGATGGGATTGACGGTCTGGGACCTAAACGCAAGCAGAATCTTATGAAGCATTTCAAGTCTTTG
ACCAAAATCAAGGAAGCCAGTGTGGATGAGATTGTCGAAGTTGGGGTACCTAGAGTCGTTGCAGAGGCTGTGCAAAGAAA
GTTGAACCCGCAGGGAGAAGCCTTGCCTCAAGTAGCAGAAGAAAGAGTAGATTACCAAACGGAAGGAAACCACAATGAAC
CATAA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(196-228)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(372-523)

GIY-YIG catalytic domain (GIY-YIG)

(17-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97S07

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae D39

99.511

100

0.995
  uvrC Streptococcus pneumoniae R6

99.511

100

0.995

References

[1] H Tettelin et al. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science (New York, N.Y.) 293(5529):498-506. [PMID: 11463916]