Detailed information    

experimental Experimentally validated

Overview

Name   uvrC   Type   Machinery gene
Locus tag   SPD_RS02885 Genome accession   NC_008533
Coordinates   551122..552966 (+) Length   614 a.a.
NCBI ID   WP_001061169.1    Uniprot ID   Q8DQQ9
Organism   Streptococcus pneumoniae D39     
Function   homologous recombination   
Homologous recombination

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.


Genomic Context

Location: 546122..557966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPD_RS02865 (SPD_0534) - 546959..547738 (+) 780 WP_000290025.1 alpha/beta hydrolase -
  SPD_RS02870 (SPD_0535) murM 547850..549070 (+) 1221 WP_001819885.1 peptidoglycan bridge formation alanyltransferase MurM -
  SPD_RS02875 (SPD_0536) murN 549075..550307 (+) 1233 WP_001229509.1 peptidoglycan bridge formation alanyltransferase MurN -
  SPD_RS02880 (SPD_0537) - 550386..551082 (+) 697 Protein_542 M57 family metalloprotease -
  SPD_RS02885 (SPD_0538) uvrC 551122..552966 (+) 1845 WP_001061169.1 excinuclease ABC subunit UvrC Machinery gene
  SPD_RS02890 (SPD_0539) - 552956..553789 (+) 834 WP_001015782.1 metallophosphoesterase family protein -
  SPD_RS02895 (SPD_0540) - 553971..554771 (-) 801 WP_000714633.1 amino acid ABC transporter substrate-binding protein -
  SPD_RS02900 (SPD_0541) - 554942..555547 (+) 606 WP_000670172.1 nitroreductase family protein -
  SPD_RS02905 (SPD_0542) pepV 555560..556960 (+) 1401 WP_000560281.1 dipeptidase PepV -
  SPD_RS02910 (SPD_0543) - 557014..557592 (+) 579 WP_000078140.1 uracil-DNA glycosylase family protein -

Sequence

Protein


Download         Length: 614 a.a.        Molecular weight: 70456.95 Da        Isoelectric Point: 9.1183

>NTDB_id=692 SPD_RS02885 WP_001061169.1 551122..552966(+) (uvrC) [Streptococcus pneumoniae D39]
MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSYFRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEIN
LIKENKPKYNIMLKDDKSYPFIKITNERYPRLIITRQVKKDGSLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERAAEYRDLIQAIGTLRTKQRVMAK
DLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNLFPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKAL
VDSKILKPQRGEKKQLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGTSPVSAMVV
FVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLIVIDGGQGQVNIAKQVIQEELGLDIPIAGLQ
KNDKHQTHELLFGDPLEVVDLSRNSQEFFLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSL
TKIKEASVDEIVEVGVPRVVAEAVQRKLNPQGEALSQVAEERVDYQTEGNHNEP

Nucleotide


Download         Length: 1845 bp        

>NTDB_id=692 SPD_RS02885 WP_001061169.1 551122..552966(+) (uvrC) [Streptococcus pneumoniae D39]
ATGAACAACTTGATTAAATCAAAACTAGAGCTCTTGCCGACCAGCCCTGGTTGCTACATTCATAAGGATAAAAATGGCAC
CATTATCTATGTAGGAAAGGCTAAAAATCTGCGTAATCGAGTACGGTCCTATTTTCGTGGAAGTCATGATACCAAGACAG
AGGCTCTGGTGTCTGAAATTGTGGATTTTGAATTTATTGTTACGGAGTCTAATATTGAGGCACTTCTCCTAGAAATCAAC
CTGATCAAGGAAAACAAGCCCAAGTACAATATCATGCTCAAGGATGACAAGTCCTATCCTTTCATCAAAATCACCAATGA
GCGCTATCCACGCTTGATTATCACTCGTCAGGTCAAAAAGGACGGAAGTCTTTATTTTGGACCCTATCCCGATGTGGGGG
CAGCCAATGAAATCAAGCGGTTGCTGGATCGGATATTCCCTTTTCGTAAGTGTACCAACCCGCCCTCTAAGGTCTGTTTT
TATTACCATATCGGTCAATGCATGGCCCACACCATCTGTAAGAAGGATGAGGCTTATTTCAAGTCTATGGCTCAGGAGGT
GTCTGATTTTCTGAAAGGTCAGGATGACAAAATCATCGATGATCTCAAGAGTAAAATGGCAGTAGCAGCACAAAGTATGG
AGTTTGAACGTGCGGCGGAATACCGTGACCTGATTCAGGCTATTGGAACGCTTCGAACCAAGCAACGGGTCATGGCGAAA
GATTTGCAAAATCGCGATGTCTTTGGCTACTATGTGGATAAGGGCTGGATGTGTGTGCAGGTTTTCTTTGTCCGTCAGGG
TAAGCTTATCGAGCGCGATGTCAATCTCTTCCCCTACTTCAATGATCCAGATGAGGATTTTTTGACCTATGTAGGACAAT
TCTATCAAGAAAAATCTCATCTAGTTCCCAATGAGGTACTGATTCCGCAGGATATTGACGAAGAGGCTGTCAAGGCCTTG
GTGGATTCCAAGATTCTTAAGCCTCAACGTGGAGAGAAAAAACAACTGGTCAATCTAGCCATAAAAAATGCTCGTGTTAG
TCTAGAGCAGAAGTTCAATCTGCTAGAAAAATCTGTCGAAAAGACTCAAGGAGCTATTGAAAATCTAGGGCGTTTGCTCC
AAATCCCGACCCCAGTACGTATCGAGTCCTTCGATAACTCTAATATCATGGGAACTAGCCCTGTTTCGGCTATGGTGGTC
TTTGTCAACGGTAAACCGAGTAAGAAGGATTACCGTAAGTACAAGATAAAAACGGTTGTTGGACCAGACGACTATGCCAG
CATGAGAGAGGTCATTCGCAGACGCTATGGTCGAGTACAGCGTGAGGCTTTGACTCCTCCAGATTTGATTGTGATTGATG
GGGGGCAAGGTCAAGTCAATATCGCTAAGCAGGTTATCCAAGAGGAACTGGGCTTGGATATTCCAATTGCTGGGCTACAA
AAGAATGATAAGCACCAAACCCATGAATTGCTCTTTGGAGATCCGCTTGAGGTGGTGGACTTGTCTCGCAATTCTCAGGA
ATTTTTCCTCCTCCAACGCATCCAAGATGAGGTGCACCGCTTTGCTATCACTTTCCACCGCCAACTGCGCTCCAAAAATT
CTTTCTCATCTCAATTGGATGGGATTGATGGTCTGGGACCTAAACGCAAGCAAAATCTCATGAGGCATTTCAAGTCTTTG
ACCAAAATCAAGGAAGCCAGTGTGGATGAGATTGTCGAAGTTGGGGTACCTAGAGTCGTTGCAGAGGCTGTGCAAAGAAA
GTTGAACCCGCAGGGAGAAGCCTTGTCTCAAGTAGCAGAAGAAAGAGTAGATTACCAAACGGAAGGAAACCACAATGAAC
CATAA

Domains

Predicted by InterProScan.

GIY-YIG catalytic domain (GIY-YIG)

(17-90)

UvrB/uvrC motif (UVR)

(196-228)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(372-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DQQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae R6

100

100

1
  uvrC Streptococcus pneumoniae TIGR4

99.511

100

0.995

References

[1] Joel A Lanie et al. (2007) Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6. Journal of Bacteriology 189(1):38-51. [PMID: 17041037]