Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DBQ26_RS17790 Genome accession   NZ_CP028574
Coordinates   3360716..3362428 (+) Length   570 a.a.
NCBI ID   WP_032005672.1    Uniprot ID   A0A9X3QFS9
Organism   Acinetobacter pittii strain WCHAP005046     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3355716..3367428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBQ26_RS17755 (DBQ26_17755) nusA 3356604..3358088 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  DBQ26_RS17760 (DBQ26_17760) rimP 3358126..3358650 (-) 525 WP_057089886.1 ribosome maturation factor RimP -
  DBQ26_RS17780 (DBQ26_17780) secG 3359288..3359617 (-) 330 WP_032005670.1 preprotein translocase subunit SecG -
  DBQ26_RS17785 (DBQ26_17785) tpiA 3359630..3360424 (-) 795 WP_017481951.1 triose-phosphate isomerase -
  DBQ26_RS17790 (DBQ26_17790) pilB 3360716..3362428 (+) 1713 WP_032005672.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DBQ26_RS17795 (DBQ26_17795) pilC 3362455..3363681 (+) 1227 WP_032005674.1 type II secretion system F family protein Machinery gene
  DBQ26_RS17800 (DBQ26_17800) pilD 3363681..3364541 (+) 861 WP_032005676.1 prepilin peptidase Machinery gene
  DBQ26_RS17805 (DBQ26_17805) coaE 3364543..3365139 (+) 597 WP_032005677.1 dephospho-CoA kinase -
  DBQ26_RS17810 (DBQ26_17810) - 3365136..3366050 (-) 915 WP_032005679.1 DMT family transporter -
  DBQ26_RS17815 (DBQ26_17815) rlmB 3366086..3366835 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  DBQ26_RS17820 (DBQ26_17820) - 3366938..3367264 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63542.90 Da        Isoelectric Point: 5.0271

>NTDB_id=285598 DBQ26_RS17790 WP_032005672.1 3360716..3362428(+) (pilB) [Acinetobacter pittii strain WCHAP005046]
MSVLNTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTNQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=285598 DBQ26_RS17790 WP_032005672.1 3360716..3362428(+) (pilB) [Acinetobacter pittii strain WCHAP005046]
ATGTCAGTCTTAAATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTGGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCAGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGACACAAACCAAATT
CCCAAAGATTTGGTTGATCAGAAACTTGTTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACTAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGCAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCACAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTAGCTTCT
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCCATCGATTTTCGTGTGAACTCACTTCCAACTTTGTTTGGTGAAAAATTGGTTCTGCGTATTCTAGATCCAT
CTAGCGCAATGCTCGGCATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACTGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTGGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGGGTTACTTCT
TTACAAGAAGTCAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

50.783

100

0.512
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.655

98.596

0.361

Multiple sequence alignment