Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1A23_RS04010 Genome accession   NZ_CP028568
Coordinates   475475..477181 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila strain WCHAH045096     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470475..482181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1A23_RS03980 (C1A23_04065) mutT 471151..471564 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  C1A23_RS03985 (C1A23_04070) yacG 471625..471819 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  C1A23_RS03990 (C1A23_04075) zapD 471829..472551 (-) 723 WP_024945431.1 cell division protein ZapD -
  C1A23_RS03995 (C1A23_04080) coaE 472589..473203 (-) 615 WP_101614270.1 dephospho-CoA kinase -
  C1A23_RS04000 (C1A23_04085) pilD 473223..474095 (-) 873 WP_101614271.1 A24 family peptidase Machinery gene
  C1A23_RS04005 (C1A23_04090) pilC 474118..475359 (-) 1242 WP_101614272.1 type II secretion system F family protein Machinery gene
  C1A23_RS04010 (C1A23_04095) pilB 475475..477181 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C1A23_RS04015 (C1A23_04100) - 477188..477619 (-) 432 WP_041216993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C1A23_RS04025 (C1A23_04110) nadC 477942..478799 (-) 858 WP_101614274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1A23_RS04030 (C1A23_04115) - 478805..479275 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  C1A23_RS04035 (C1A23_04120) ampD 479422..479994 (+) 573 WP_101614275.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1A23_RS04040 (C1A23_04125) pdhR 480270..481034 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=285541 C1A23_RS04010 WP_029302067.1 475475..477181(-) (pilB) [Aeromonas hydrophila subsp. hydrophila strain WCHAH045096]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=285541 C1A23_RS04010 WP_029302067.1 475475..477181(-) (pilB) [Aeromonas hydrophila subsp. hydrophila strain WCHAH045096]
ATGACCTCCAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAAGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATTGATGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGGTCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCGCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCTATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGAATCTTGCATGAAATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTTATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCGGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAAC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGCTCGGGTGGATACAAAGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAAGAAGGCATGCGTACCTTGCGCACCTCCGGCCTCGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment