Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG55_RS13405 Genome accession   NZ_CP028561
Coordinates   2496605..2498320 (+) Length   571 a.a.
NCBI ID   WP_087536894.1    Uniprot ID   A0A2R4TMP3
Organism   Acinetobacter sp. WCHA45     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491605..2503320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG55_RS13370 (CDG55_13360) nusA 2492460..2493944 (-) 1485 WP_005218583.1 transcription termination factor NusA -
  CDG55_RS13375 (CDG55_13365) rimP 2493982..2494506 (-) 525 WP_087536896.1 ribosome maturation factor RimP -
  CDG55_RS13395 (CDG55_13385) secG 2495129..2495458 (-) 330 WP_005162938.1 preprotein translocase subunit SecG -
  CDG55_RS13400 (CDG55_13390) tpiA 2495471..2496262 (-) 792 WP_087536895.1 triose-phosphate isomerase -
  CDG55_RS13405 (CDG55_13395) pilB 2496605..2498320 (+) 1716 WP_087536894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG55_RS13410 (CDG55_13400) pilC 2498344..2499570 (+) 1227 WP_087536893.1 type II secretion system F family protein Machinery gene
  CDG55_RS15600 (CDG55_13405) pilD 2499570..2500430 (+) 861 WP_087536892.1 prepilin peptidase Machinery gene
  CDG55_RS13420 (CDG55_13410) coaE 2500432..2501037 (+) 606 WP_087536891.1 dephospho-CoA kinase -
  CDG55_RS13425 (CDG55_13415) - 2501034..2501945 (-) 912 WP_087536890.1 DMT family transporter -
  CDG55_RS13430 (CDG55_13420) rlmB 2501976..2502725 (-) 750 WP_005162952.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG55_RS13435 (CDG55_13425) - 2502905..2503231 (-) 327 WP_004659291.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63205.50 Da        Isoelectric Point: 5.3071

>NTDB_id=285524 CDG55_RS13405 WP_087536894.1 2496605..2498320(+) (pilB) [Acinetobacter sp. WCHA45]
MSGLHTSPKFSGFIRRLVEDGHMTAANMQNAIDAAKKAKQDIVAHLIEQHRLSPLTIAESISVEFGEPLFDLAVYDVGLL
PRELTDNKLIQKHRVVPLTQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHTKLEKLIEQHFGDSSSFDFGDSED
FDLDIDVNQDSPQEDDDASPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQMATRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDHQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPVDVPKQSLIELG
FTEQDLANPDLQIFQPIGCAECREGYKGRVGIYEVMKVTPAISQIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=285524 CDG55_RS13405 WP_087536894.1 2496605..2498320(+) (pilB) [Acinetobacter sp. WCHA45]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTCATTCGACGTTTAGTTGAAGATGGTCATATGACTGCTGCAAA
TATGCAGAATGCAATTGATGCAGCCAAGAAAGCCAAACAAGATATTGTTGCTCATTTAATCGAACAACATCGTCTTTCTC
CACTTACCATTGCAGAAAGCATCTCTGTAGAATTTGGCGAACCACTTTTTGATTTGGCGGTTTACGATGTTGGATTATTA
CCTCGAGAATTAACCGACAATAAACTCATACAAAAGCATCGTGTTGTTCCGTTAACACAACGTGGACAAATTTTGTATGT
TGCTACGAGCAATCCAACCAATATTGATGCATTAGATGCAATTCGTTTCAATACCAAACTTAATATTGAACCAATTATTG
TTGAGCATACTAAACTTGAAAAGTTAATCGAACAACATTTTGGTGATTCAAGTAGTTTCGATTTTGGTGACAGTGAGGAC
TTCGACTTAGATATTGATGTTAATCAAGATTCACCTCAAGAAGATGATGATGCATCACCTCAAGGTGACGAATCCCCAAT
TGTCAAATATATTAATAAGCTTTTAATTGATGCAATTCGCATGGGCGCATCAGATTTACATTTTGAGCCCTATGAAAAAA
TGTACCGTGTGCGTTATCGTGTTGATGGTGTGCTCCGTCAAATCGCTACACCTCCTTTACAAATGGCAACCCGCTTAGCT
TCTCGTTTAAAAGTAATGTCTCAAATGGATATCTCTGAAAAACGAATGCCTCAAGATGGTCGTATTAAACTCAAATTGTC
AAAAACCAAAGCCATCGATTTCCGTGTAAACTCTTTACCCACACTGTTTGGGGAAAAATTAGTACTACGTATTTTGGATC
CATCAAGCGCTATGCTCGGGATTGAAGCATTAGGCTACGAAGATCATCAGAAAGCATTATTCATGGAAGCACTTGAAAAA
CCTCAAGGAATGCTACTGATTACAGGTCCAACAGGTTCTGGTAAAACCGTATCGCTATATACTGGTCTAAATATTCTAAA
CACTGAGGATACTAATATTTCAACAGCGGAAGATCCTGTTGAAATTAACTTAGAAGGCATCAACCAAGTCAATGTGAATC
CCAAAACGGGGTTAACTTTCTCTGCTGCACTGAAAGCTTTTCTACGTCAAGATCCAGATGTAATCATGGTCGGTGAGATT
CGTGACCTTGAAACTGCTGAAATTGCAATTAAAGCAGCTCAGACAGGTCATATGGTAATGTCAACATTACACACCAACAG
CGCACCTGAAACTTTAACTCGTTTGCGTAATATGGGGGTACCCTCATTCAATATTGCAACCTCAGTCAATCTTGTCATTG
CACAACGTTTAGCACGACGCTTATGTTCACAATGTAAAGCACCCGTAGATGTACCTAAACAAAGTCTCATTGAACTTGGA
TTTACTGAACAAGACTTAGCCAATCCGGATTTACAGATCTTTCAACCGATTGGCTGTGCGGAATGTCGAGAAGGGTACAA
AGGACGTGTAGGTATTTATGAGGTGATGAAGGTCACTCCTGCAATCTCTCAAATCATCATGGAAGATGGTAATGCACTAC
AAATTGCAGCGGCCTCAGAAAAAGCTGGTTTTGATAATCTGCGTCGCTCAGGACTAAAAAAAGTAATGCAAGGAATTACC
TCCTTGCAAGAAATTAACCGCGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R4TMP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.487

100

0.825
  pilB Acinetobacter baylyi ADP1

77.233

100

0.772
  pilB Legionella pneumophila strain ERS1305867

54.328

95.096

0.517
  pilF Neisseria gonorrhoeae MS11

48.348

100

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

49.464

98.074

0.485
  pilB Vibrio campbellii strain DS40M4

48.658

97.898

0.476
  pilB Vibrio cholerae strain A1552

51.064

90.543

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.477

100

0.37
  pilF Thermus thermophilus HB27

37.5

98.074

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

92.995

0.361

Multiple sequence alignment