Detailed information NTG0285536

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CDG55_RS15600
• Genome accession: NZ_CP028561
• Coordinates: 2499570..2500430 (+)
• Length: 286 a.a.
• NCBI ID: WP_087536892.1
• Uniprot ID: A0A2R4TMI2
• Organism: Acinetobacter sp. WCHA45
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2494570..2505430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDG55_RS13395 (CDG55_13385)	secG	2495129..2495458 (-)	330	WP_005162938.1	preprotein translocase subunit SecG	-
CDG55_RS13400 (CDG55_13390)	tpiA	2495471..2496262 (-)	792	WP_087536895.1	triose-phosphate isomerase	-
CDG55_RS13405 (CDG55_13395)	pilB	2496605..2498320 (+)	1716	WP_087536894.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CDG55_RS13410 (CDG55_13400)	pilC	2498344..2499570 (+)	1227	WP_087536893.1	type II secretion system F family protein	Machinery gene
CDG55_RS15600 (CDG55_13405)	pilD	2499570..2500430 (+)	861	WP_087536892.1	prepilin peptidase	Machinery gene
CDG55_RS13420 (CDG55_13410)	coaE	2500432..2501037 (+)	606	WP_087536891.1	dephospho-CoA kinase	-
CDG55_RS13425 (CDG55_13415)	-	2501034..2501945 (-)	912	WP_087536890.1	DMT family transporter	-
CDG55_RS13430 (CDG55_13420)	rlmB	2501976..2502725 (-)	750	WP_005162952.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
CDG55_RS13435 (CDG55_13425)	-	2502905..2503231 (-)	327	WP_004659291.1	pyrimidine/purine nucleoside phosphorylase	-
CDG55_RS13440 (CDG55_13430)	recN	2503574..2505232 (+)	1659	WP_087536889.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31823.25 Da
• Isoelectric Point: 7.4712

>NTDB_id=285536 CDG55_RS15600 WP_087536892.1 2499570..2500430(+) (pilD) [Acinetobacter sp. WCHA45]
MLEIISYFIQNPTALYVAVGILSLCIGSFLNVVIFRTPKMMEQEWHQECQMLLHPEQPFIDSTKLTLSQPASTCPKCQSP
IRWYQNIPIISWVLLRGKCGACQNPISIRYPLIELLTMVCSLIVVAVFGATIQMLFGLVLTWVLITLTFIDFDTQLLPDR
FTLPLAALGLGINSFALYTTASSAIWGYLIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSV
VGAIIGIILMKIRGENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=285536 CDG55_RS15600 WP_087536892.1 2499570..2500430(+) (pilD) [Acinetobacter sp. WCHA45]
ATGCTAGAGATCATTTCTTATTTTATCCAAAATCCAACTGCATTATACGTCGCAGTCGGGATTTTGAGTCTCTGTATTGG
TAGTTTTCTTAACGTTGTTATTTTTCGAACGCCTAAAATGATGGAGCAGGAATGGCATCAGGAATGCCAAATGCTGCTTC
ATCCAGAGCAGCCATTTATTGACTCAACCAAGCTCACACTCAGCCAGCCTGCTTCGACCTGTCCAAAGTGCCAATCCCCT
ATCCGTTGGTATCAAAATATACCGATCATCAGTTGGGTTCTACTTCGTGGCAAATGCGGTGCTTGCCAGAATCCAATTAG
TATTCGCTATCCATTGATTGAACTTCTCACCATGGTTTGTTCATTAATCGTTGTAGCAGTATTTGGTGCAACCATTCAGA
TGCTCTTTGGTTTGGTCCTTACATGGGTATTGATCACACTCACCTTTATTGACTTTGATACACAACTCCTTCCTGACCGC
TTCACACTGCCATTGGCAGCACTCGGCTTGGGAATTAACAGTTTTGCACTCTACACAACTGCCAGTTCAGCAATTTGGGG
TTATTTGATTGGTTTTTTATGCTTATGGGTGGTTTACTATCTTTTTAAATTGGTCACTGGTAAAGAAGGCATGGGATATG
GTGACTTTAAGTTGCTTGCCGCTCTAGGTGCCTGGATGGGACCTATGATGCTGCCACTTATAGTTCTGCTGTCTTCAGTT
GTCGGGGCGATTATTGGCATTATTCTGATGAAAATACGCGGTGAAAACCAGCCATTTGCCTTTGGACCCTATATCGCCAT
TGCAGGCTGGATCGCATTTTTATGGGGTGATCAAATTATGAAAGTCTATTTAGGTGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A2R4TMI2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	82.517	100	0.825
pilD	Acinetobacter nosocomialis M2	82.517	100	0.825
pilD	Vibrio campbellii strain DS40M4	50.35	100	0.504
pilD	Vibrio cholerae strain A1552	52.963	94.406	0.5
pilD	Neisseria gonorrhoeae MS11	48.092	91.608	0.441