Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   C9I44_RS00660 Genome accession   NZ_CP028386
Coordinates   136429..137373 (+) Length   314 a.a.
NCBI ID   WP_003133715.1    Uniprot ID   -
Organism   Lactococcus garvieae strain IBB3403     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131429..142373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9I44_RS00645 (C9I44_00640) - 132691..134292 (-) 1602 WP_017370492.1 GH25 family lysozyme -
  C9I44_RS00650 (C9I44_00645) guaB 134461..135942 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  C9I44_RS00655 (C9I44_00650) rpsU 136065..136241 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  C9I44_RS00660 (C9I44_00655) comGA 136429..137373 (+) 945 WP_003133715.1 competence type IV pilus ATPase ComGA Machinery gene
  C9I44_RS00665 (C9I44_00660) comGB 137312..138331 (+) 1020 WP_263867708.1 competence type IV pilus assembly protein ComGB Machinery gene
  C9I44_RS00670 (C9I44_00665) - 138449..139894 (-) 1446 WP_233239261.1 recombinase family protein -
  C9I44_RS00675 (C9I44_00670) - 140017..140391 (-) 375 WP_233239262.1 PH domain-containing protein -
  C9I44_RS00680 (C9I44_00675) - 140437..140637 (-) 201 WP_233239263.1 hypothetical protein -
  C9I44_RS00685 (C9I44_00680) - 140645..140968 (-) 324 WP_233239264.1 hypothetical protein -
  C9I44_RS00690 (C9I44_00685) - 141014..141412 (-) 399 WP_233239265.1 hypothetical protein -
  C9I44_RS00695 (C9I44_00690) - 141412..141936 (-) 525 WP_233239266.1 helix-turn-helix domain-containing protein -
  C9I44_RS00700 (C9I44_00695) - 142108..142347 (+) 240 WP_233239267.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35836.07 Da        Isoelectric Point: 6.6641

>NTDB_id=284549 C9I44_RS00660 WP_003133715.1 136429..137373(+) (comGA) [Lactococcus garvieae strain IBB3403]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=284549 C9I44_RS00660 WP_003133715.1 136429..137373(+) (comGA) [Lactococcus garvieae strain IBB3403]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGGGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTATTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGATTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.603

99.363

0.513
  comYA Streptococcus mutans UA140

51.603

99.363

0.513

Multiple sequence alignment