Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7N77_RS06400 Genome accession   NZ_CP027856
Coordinates   1316890..1318596 (-) Length   568 a.a.
NCBI ID   WP_106885657.1    Uniprot ID   -
Organism   Aeromonas rivipollensis strain KN-Mc-11N1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311890..1323596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7N77_RS06370 (C7N77_06370) mutT 1312489..1312893 (+) 405 WP_106885652.1 8-oxo-dGTP diphosphatase MutT -
  C7N77_RS06375 (C7N77_06375) yacG 1312968..1313162 (-) 195 WP_025328449.1 DNA gyrase inhibitor YacG -
  C7N77_RS06380 (C7N77_06380) zapD 1313172..1313894 (-) 723 WP_162520171.1 cell division protein ZapD -
  C7N77_RS06385 (C7N77_06385) coaE 1313932..1314546 (-) 615 WP_106885654.1 dephospho-CoA kinase -
  C7N77_RS06390 (C7N77_06390) pilD 1314565..1315437 (-) 873 WP_106885655.1 prepilin peptidase Machinery gene
  C7N77_RS06395 (C7N77_06395) pilC 1315518..1316759 (-) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  C7N77_RS06400 (C7N77_06400) pilB 1316890..1318596 (-) 1707 WP_106885657.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C7N77_RS06405 (C7N77_06405) - 1318600..1319034 (-) 435 WP_106887849.1 pilin -
  C7N77_RS06415 (C7N77_06415) nadC 1319347..1320210 (-) 864 WP_106885658.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C7N77_RS06420 (C7N77_06420) - 1320214..1320684 (-) 471 WP_106885659.1 retropepsin-like aspartic protease family protein -
  C7N77_RS06425 (C7N77_06425) ampD 1320821..1321393 (+) 573 WP_106885660.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C7N77_RS06430 (C7N77_06430) pdhR 1321669..1322433 (+) 765 WP_106885661.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62522.85 Da        Isoelectric Point: 5.8194

>NTDB_id=280980 C7N77_RS06400 WP_106885657.1 1316890..1318596(-) (pilB) [Aeromonas rivipollensis strain KN-Mc-11N1]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPDAELQELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=280980 C7N77_RS06400 WP_106885657.1 1316890..1318596(-) (pilB) [Aeromonas rivipollensis strain KN-Mc-11N1]
ATGACCTCTAGCCTCAATAGCGGCCTGGCAACCAGCCTGGCCGCATCTTCCCTCCTCAGTGAGCCGGACTCGCAGCGCTA
TCTGAGCCAGGCCAGAGCGCAGCGCAAGCCCTTTGTCACTTTCCTGATTGAAAACGACATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCACCATGTCCTGCCCATCTATACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTAGAGGATTTCGGCTTCAGCTTCGGCCTGCACACTGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTTTCCGATGAGGGTTCGCGCCTCGACGAGAGCGTCAATACCGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAGTCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGACTCGATATCGCCGAGCGACGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCACGCAG
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGTGAGAAGATAGTCATCCGCTTGCTGGACTCGTCAG
CCGCCCGCCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGCTCTGGCAAGACGGTGTCGCTCTATACAGGTCTCAACATTCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAATCTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCTTGCGCTCATTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAAATACGGGAT
CTTGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACCGGTCACCTTGTGCTGTCGACCCTGCACACCAATTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GTCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCGCCCGAGGTGGTGCCCGACGCCGAACTGCAGGAGCTGGGCTTTACC
CAACAGCAGCTGGCCGCCGGACTGCGACTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCGGGAGGATACAGAGGACGAGT
CGGCATCTATGAAATCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGGGCTAATTCGCTACAAATCGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCCTGCGCACTTCCGGCCTGGAGAAGGCCCGCCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACCACGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.77

99.648

0.546
  pilB Acinetobacter baylyi ADP1

54.982

98.944

0.544
  pilB Vibrio cholerae strain A1552

53.108

99.12

0.526
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.267

97.535

0.373
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment