Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7R88_RS08740 Genome accession   NZ_CP027852
Coordinates   1970402..1971922 (+) Length   506 a.a.
NCBI ID   WP_106911397.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain MS-17-188     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1965402..1976922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7R88_RS08715 (C7R88_08705) ampE 1966125..1966967 (-) 843 WP_106911394.1 beta-lactamase regulator AmpE -
  C7R88_RS08725 (C7R88_08715) ampD 1967168..1967773 (-) 606 WP_106911395.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C7R88_RS08730 (C7R88_08720) nadC 1968210..1969136 (+) 927 WP_106911396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C7R88_RS08735 (C7R88_08725) - 1969907..1970347 (+) 441 WP_106912106.1 pilin -
  C7R88_RS08740 (C7R88_08730) pilB 1970402..1971922 (+) 1521 WP_106911397.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C7R88_RS08745 (C7R88_08735) - 1971925..1973160 (+) 1236 WP_106911398.1 type II secretion system F family protein -
  C7R88_RS08750 (C7R88_08740) - 1973317..1973871 (-) 555 WP_106911399.1 YbhB/YbcL family Raf kinase inhibitor-like protein -
  C7R88_RS08755 (C7R88_08745) - 1974088..1975128 (-) 1041 WP_010862605.1 GMP reductase -
  C7R88_RS08760 (C7R88_08750) pilD 1975416..1976252 (+) 837 WP_230425799.1 prepilin peptidase Machinery gene
  C7R88_RS08765 (C7R88_08755) coaE 1976254..1976862 (+) 609 WP_106911400.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55846.05 Da        Isoelectric Point: 6.1523

>NTDB_id=280946 C7R88_RS08740 WP_106911397.1 1970402..1971922(+) (pilB) [Plesiomonas shigelloides strain MS-17-188]
MKIENPTELKKYASFSSLITKYFALPIFDNSNTLYLGVISHDCFECIDAFRFACGKQIETTLVNKEELREAIFIVTGQYP
EDADNPMGSSGLEEDTLTQLEELADESALNDDLALSSEQDEAPIVRFINQMLLRAVRQHASDIHFEPYEKQYRVRFRVDG
VLQEIASPPDKLGARLAARLKIMSKLDIAEKRIPQDGRIKLKFKDTPAIDLRVSTLPTLFGEKVVLRILDSSAANLNIDQ
LGFTDADKQRYLSALAKPQGMILVTGPTGSGKTVTLYTGLSLLNQPERNISTAEDPVEINLAGINQTQINLKAGLTFASA
LRAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLANMGVPAYNIASSIHLIIAQRLARRLCP
ECRQPHLLTEAEQKAAGFSQCHAEGLRIFKPKGCPKCTNGYKGRTGLYEIMPITPALTDAILRGEHAPELTHIARQHGML
TLREAGLNKVREGVTSLEEIERVTGV

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=280946 C7R88_RS08740 WP_106911397.1 1970402..1971922(+) (pilB) [Plesiomonas shigelloides strain MS-17-188]
ATGAAAATAGAAAACCCTACCGAACTAAAAAAATACGCTTCTTTTTCATCGCTTATTACTAAGTATTTTGCTCTGCCGAT
ATTTGATAACAGCAACACGCTATATCTTGGTGTCATCAGCCATGATTGTTTTGAATGTATTGATGCGTTTCGTTTTGCTT
GCGGCAAACAAATTGAGACCACTCTGGTTAATAAAGAAGAGCTGCGCGAGGCCATTTTCATCGTCACCGGTCAATACCCA
GAAGATGCTGACAATCCAATGGGCAGCTCAGGGCTAGAAGAAGACACCCTAACTCAGCTTGAAGAGTTGGCGGACGAAAG
CGCGCTCAATGATGATCTAGCCTTAAGCAGTGAGCAAGATGAAGCCCCTATCGTGCGCTTTATCAACCAAATGCTGCTAC
GCGCAGTGCGCCAACACGCCTCCGATATTCATTTTGAGCCCTATGAAAAGCAGTATCGCGTCCGCTTTCGGGTTGATGGC
GTATTGCAAGAGATCGCCAGCCCACCGGATAAGCTCGGTGCTCGCTTGGCAGCACGCCTAAAAATCATGAGTAAGTTGGA
TATTGCCGAAAAGCGGATCCCGCAAGATGGTCGTATCAAGCTCAAATTTAAAGACACACCGGCTATCGATCTGCGCGTTT
CTACGCTGCCCACCCTATTTGGTGAAAAAGTAGTACTGCGGATCCTCGACTCCTCTGCTGCCAACTTAAATATTGATCAG
CTCGGGTTTACTGATGCCGATAAACAGCGCTATTTGAGCGCCTTAGCTAAGCCGCAAGGCATGATCTTAGTCACCGGCCC
GACCGGTAGCGGTAAAACCGTGACCTTGTATACCGGTTTGTCATTACTCAATCAGCCAGAGCGCAATATCTCTACCGCCG
AAGATCCGGTGGAGATTAACTTAGCCGGTATCAACCAAACCCAGATCAACCTCAAAGCCGGCCTGACGTTCGCCAGCGCA
TTGCGCGCCTTTTTACGGCAAGATCCTGACGTGGTAATGGTGGGGGAAATTCGCGATCTGGAAACCGCTGAAATTGCGGT
AAAAGCGGCGCAAACCGGCCACTTAGTACTGTCGACGCTGCACACCAATTCTGCCGCCGAAACCCTCACTCGACTGGCCA
ATATGGGCGTTCCGGCCTATAACATTGCCTCATCAATTCATCTGATCATCGCCCAGCGATTGGCTCGCCGCCTCTGCCCC
GAGTGTCGCCAGCCACATCTGCTGACCGAAGCCGAACAAAAAGCTGCTGGCTTTAGCCAATGCCACGCCGAAGGATTACG
CATTTTTAAACCCAAGGGCTGCCCAAAATGTACCAACGGCTACAAAGGGCGAACCGGCTTATATGAAATTATGCCAATCA
CCCCAGCACTGACCGATGCCATTTTACGCGGTGAGCATGCGCCAGAGTTAACCCACATTGCACGCCAACATGGCATGCTG
ACCTTGCGTGAAGCTGGTCTGAATAAAGTGCGTGAAGGCGTAACATCATTGGAAGAAATTGAACGGGTTACAGGAGTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

57.172

96.443

0.551
  pilB Vibrio cholerae strain A1552

56.735

96.838

0.549
  pilB Vibrio campbellii strain DS40M4

56.879

96.245

0.547
  pilB Acinetobacter baumannii D1279779

53.971

97.036

0.524
  pilB Acinetobacter baylyi ADP1

53.469

96.838

0.518
  pilB Legionella pneumophila strain ERS1305867

52.352

96.64

0.506
  pilF Neisseria gonorrhoeae MS11

56.298

76.877

0.433
  pilB Haemophilus influenzae Rd KW20

39.721

99.012

0.393
  pilB Haemophilus influenzae 86-028NP

39.521

99.012

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.918

96.047

0.383
  pilB Glaesserella parasuis strain SC1401

37.5

99.605

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

44.769

81.225

0.364

Multiple sequence alignment