Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C7K70_RS02220 Genome accession   NZ_CP027804
Coordinates   460120..461826 (-) Length   568 a.a.
NCBI ID   WP_106551645.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain KN-Mc-1R2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455120..466826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7K70_RS02190 (C7K70_02190) - 455431..456241 (-) 811 Protein_414 IS1595 family transposase -
  C7K70_RS02195 (C7K70_02195) yacG 456270..456464 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  C7K70_RS02200 (C7K70_02200) zapD 456474..457196 (-) 723 WP_024945431.1 cell division protein ZapD -
  C7K70_RS02205 (C7K70_02205) coaE 457234..457848 (-) 615 WP_106551643.1 dephospho-CoA kinase -
  C7K70_RS02210 (C7K70_02210) pilD 457868..458740 (-) 873 WP_106551644.1 A24 family peptidase Machinery gene
  C7K70_RS02215 (C7K70_02215) pilC 458763..460004 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  C7K70_RS02220 (C7K70_02220) pilB 460120..461826 (-) 1707 WP_106551645.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C7K70_RS23490 - 461830..462261 (-) 432 WP_106551646.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C7K70_RS02235 (C7K70_02235) nadC 462584..463441 (-) 858 WP_106551648.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C7K70_RS02240 (C7K70_02240) - 463447..463917 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  C7K70_RS02245 (C7K70_02245) ampD 464065..464637 (+) 573 WP_106551649.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C7K70_RS02250 (C7K70_02250) pdhR 464913..465677 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62527.90 Da        Isoelectric Point: 5.4342

>NTDB_id=280835 C7K70_RS02220 WP_106551645.1 460120..461826(-) (pilB) [Aeromonas hydrophila strain KN-Mc-1R2]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=280835 C7K70_RS02220 WP_106551645.1 460120..461826(-) (pilB) [Aeromonas hydrophila strain KN-Mc-1R2]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
CCTGAGTCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCTACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGACTCGATGAGAGCGTCAATACCACCGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAACGCGGGGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGTTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCAG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGTGTGACCAGTCTAGCG
GAGATAAACCGGGTAACTACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment