Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K00_RS04155 Genome accession   NZ_CP027757
Coordinates   927428..928636 (-) Length   402 a.a.
NCBI ID   WP_124411522.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain R2-4-08W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922428..933636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K00_RS04120 (C4K00_0818) - 923728..923901 (-) 174 WP_124399316.1 DUF3094 family protein -
  C4K00_RS04125 (C4K00_0819) - 923990..924619 (-) 630 WP_043049309.1 DUF1780 domain-containing protein -
  C4K00_RS04130 (C4K00_0820) - 924782..925471 (+) 690 WP_124399317.1 energy-coupling factor ABC transporter permease -
  C4K00_RS04135 (C4K00_0821) - 925536..925751 (+) 216 WP_003171680.1 hypothetical protein -
  C4K00_RS04140 (C4K00_0822) yacG 925748..925948 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  C4K00_RS04145 (C4K00_0823) coaE 925945..926568 (-) 624 WP_124399318.1 dephospho-CoA kinase -
  C4K00_RS04150 (C4K00_0824) pilD 926565..927431 (-) 867 WP_124399319.1 prepilin peptidase Machinery gene
  C4K00_RS04155 (C4K00_0825) pilC 927428..928636 (-) 1209 WP_124411522.1 type II secretion system F family protein Machinery gene
  C4K00_RS04160 (C4K00_0826) - 928837..929250 (+) 414 WP_124399321.1 pilin -
  C4K00_RS04170 (C4K00_0828) - 929538..929843 (+) 306 WP_124399322.1 DUF6388 family protein -
  C4K00_RS04175 (C4K00_0829) nadC 929963..930811 (-) 849 WP_124399323.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C4K00_RS04180 (C4K00_0830) - 931057..933204 (+) 2148 WP_124411523.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43518.00 Da        Isoelectric Point: 10.0984

>NTDB_id=280189 C4K00_RS04155 WP_124411522.1 927428..928636(-) (pilC) [Pseudomonas synxantha strain R2-4-08W]
MNDTSTIYAWEGINRKGRRVSGRIAGHDLARVKAQLRQQGICPERVCKKPTSRPIFAPRILPADIALFTRQLATLLKAGI
PLLQAFDIISEGVENRQVRDLVKALKQQIASGNSLADALRQHPRYFDDLYCNLVATGEQAGALESLLERIAIHLEKIQRL
KARIKKAMTYPVAVLVVATLVSAVLLIHVVPQFQNLFAGVDSQLPGFTLAVIALSEFLQQAWWMLALGLGASLAGLRQAY
RAYPGFRHQLQAGLLKIPLAGKLLHNSAVARYARTLSTTVAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QSMAGSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDTLVDNLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=280189 C4K00_RS04155 WP_124411522.1 927428..928636(-) (pilC) [Pseudomonas synxantha strain R2-4-08W]
ATGAATGACACGTCAACGATCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCGAATAGCTGGCCA
TGACCTTGCGCGGGTCAAGGCGCAACTGCGCCAGCAAGGGATCTGCCCCGAGCGTGTGTGCAAGAAGCCTACATCACGGC
CGATCTTTGCCCCACGAATATTGCCTGCGGATATCGCCCTGTTCACCCGCCAGTTGGCCACATTGCTTAAGGCCGGCATC
CCGCTTCTGCAAGCCTTCGACATCATCAGCGAGGGCGTCGAGAACCGGCAGGTGCGTGATCTGGTCAAAGCCTTGAAACA
GCAGATCGCCAGCGGCAACAGCTTGGCCGATGCACTGCGCCAACATCCCCGTTATTTCGATGACCTTTACTGCAACCTGG
TGGCCACCGGGGAACAGGCCGGCGCCCTGGAATCGCTGCTGGAGCGAATAGCGATTCACCTGGAGAAAATCCAACGGCTC
AAAGCCAGGATCAAAAAGGCCATGACCTACCCTGTCGCGGTACTGGTGGTTGCCACACTGGTCAGCGCGGTGCTGTTGAT
ACACGTAGTGCCGCAATTCCAGAACCTGTTCGCCGGCGTCGACAGCCAATTGCCCGGTTTTACCTTGGCGGTCATTGCCC
TTTCCGAATTCCTGCAACAGGCGTGGTGGATGCTGGCGCTGGGCTTGGGTGCATCGCTGGCAGGGCTGCGCCAGGCTTAC
CGTGCTTACCCAGGCTTTCGCCATCAGCTGCAAGCGGGTTTGTTGAAAATACCCCTGGCAGGCAAACTGCTGCACAACTC
TGCCGTCGCTCGTTACGCCCGCACGCTTTCAACCACGGTTGCAGCGGGCGTGCCCCTTGTGCAGGCATTGGGCTCGGTGG
CCGGCGCCGTCGGCAATGGACCATTCAAACAGGCAATTGAACGTATGCGCCACGATGTATCCACAGGCATGCAATTGAAC
CAGTCCATGGCCGGCAGCGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCCATCGGTGAAGAATCCGGCACGCTGGA
CCGGATGTTGGAAAAAGTCGCGAACCACTACGAGGCGGACGTGGACACCTTGGTCGATAACCTCACAAGCCTGATGGAAC
CGCTGATCATGGTGGTACTGGGGGGCATTGTCGGGGCACTGGTGATCGCCATGTACCTGCCGGTCTTTCAACTGGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.538

99.005

0.57
  pilC Acinetobacter baylyi ADP1

50.253

98.507

0.495
  pilC Legionella pneumophila strain ERS1305867

48.363

98.756

0.478
  pilC Acinetobacter baumannii D1279779

47.848

98.259

0.47
  pilG Neisseria gonorrhoeae MS11

40.157

94.776

0.381
  pilC Thermus thermophilus HB27

37.905

99.751

0.378
  pilG Neisseria meningitidis 44/76-A

39.37

94.776

0.373
  pilC Vibrio cholerae strain A1552

37.5

97.512

0.366
  pilC Vibrio campbellii strain DS40M4

36.99

97.512

0.361

Multiple sequence alignment