Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4K02_RS02270 Genome accession   NZ_CP027755
Coordinates   506496..507821 (+) Length   441 a.a.
NCBI ID   WP_043047235.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain 2-79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501496..512821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K02_RS02250 (C4K02_0461) - 501559..504024 (-) 2466 WP_043047239.1 penicillin-binding protein 1A -
  C4K02_RS29510 (C4K02_0462) - 504021..504158 (-) 138 WP_161796498.1 hypothetical protein -
  C4K02_RS02255 (C4K02_0463) pilM 504254..504910 (+) 657 WP_043047238.1 type IV pilus assembly protein PilM -
  C4K02_RS02260 (C4K02_0464) - 504907..505458 (+) 552 WP_043047237.1 PilN domain-containing protein -
  C4K02_RS02265 (C4K02_0465) - 505455..506426 (+) 972 WP_043047236.1 pilus assembly protein PilP -
  C4K02_RS02270 (C4K02_0466) pilQ 506496..507821 (+) 1326 WP_043047235.1 type IV pilus secretin PilQ family protein Machinery gene
  C4K02_RS02275 (C4K02_0467) aroK 507826..508344 (+) 519 WP_003187914.1 shikimate kinase AroK -
  C4K02_RS02280 (C4K02_0468) aroB 508470..509570 (+) 1101 WP_043047234.1 3-dehydroquinate synthase -
  C4K02_RS02285 (C4K02_0469) - 509581..511152 (+) 1572 WP_043047233.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47996.93 Da        Isoelectric Point: 4.8746

>NTDB_id=280139 C4K02_RS02270 WP_043047235.1 506496..507821(+) (pilQ) [Pseudomonas synxantha strain 2-79]
MAAAVPNRPDLIHLPPPDRALPASYTGEKLTLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGSITLRLKNVPWDQALDLV
LQTKGLDKRVQAGVLLVAPADELAARELLVLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSMTGFEGATDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGISKPLADGAEPPA
NPPSSPFVDLGSLTGTSGLGIAYITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=280139 C4K02_RS02270 WP_043047235.1 506496..507821(+) (pilQ) [Pseudomonas synxantha strain 2-79]
ATGGCTGCCGCTGTGCCCAATCGCCCGGACCTGATCCACCTGCCGCCGCCTGACCGTGCCTTGCCGGCCAGCTATACGGG
CGAAAAATTGACCCTCAATTTCCAGGACATCGACCTGCGCGCGGTATTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGCGACGAGGTACAGGGTTCGATCACCCTGCGGCTCAAAAATGTGCCCTGGGATCAGGCGCTGGACCTGGTG
CTGCAAACCAAGGGCCTGGACAAACGGGTGCAGGCCGGCGTGTTGCTGGTGGCGCCCGCCGATGAATTGGCCGCTCGCGA
ACTGCTGGTGCTGGAGTCGCGCAAGCAGATGGCCGAGCTGGCACCGTTGCGTCGAGAGTTATTGCAGGTCAATTACGCCA
AGGCTGCGGACCTGGCCAAGCTATTCCAGTCGATGACCGGATTTGAAGGTGCCACGGATGAGCGCGGCTCGGTGGCCGTG
GATGACCGCACCAACAACATCATCGCCTACCAGACCGGCGAGCGCCTCGAAGAGCTGCGGCGCATCGTGGCGCAACTGGA
TATCCCGGTACGCCAGGTGATGATCGAGGCGCGAATCGTTGAGGCCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCT
GGGGTGGGCGGCTCAGCCGCGGCAACTGGGGCGCGGGGGGTATTTCAAAACCCCTGGCGGACGGCGCCGAGCCCCCGGCA
AACCCGCCCAGTTCACCCTTCGTCGACCTGGGTTCGCTGACCGGTACGTCGGGGCTGGGCATCGCCTACATTACCGACAA
CCTGTTGCTGGATCTTGAGCTGACGGCGATGGAGAAAAGCGGCAATGGCGAAATAGTTTCGCAGCCCAAGGTGGTCACGT
CCGATAAAGAGACCGCACGCATCCTCAAGGGCACCGAGATTCCCTATCAGGAGTCCACCTCCCAGGGCGCTACCTCGGTG
TCGTTCAAGGAAGCGTCCCTTTCACTGGAGGTCACGCCGCAGATCACCCCCGATGACCACGTGATCATGGCGGTCAAGGT
CACCAAGGACGAGCCCGACTACCTGAACCGGCTCAATGACGTGCCGCCGATCAAGAAAAACGAAGTCAATGCCAAGGTAC
TGGTCCGGGATGGCGAGACCATCGTCATTGGTGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.625

99.093

0.71
  comQ Acinetobacter baylyi ADP1

43.681

100

0.447
  pilQ Acinetobacter baumannii D1279779

39.351

100

0.44
  pilQ Acinetobacter baumannii strain A118

39.351

100

0.44
  pilQ Vibrio campbellii strain DS40M4

43.649

98.186

0.429
  pilQ Legionella pneumophila strain Lp02

44.524

95.238

0.424
  pilQ Legionella pneumophila strain ERS1305867

44.048

95.238

0.42
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.023

97.506

0.419
  pilQ Vibrio cholerae strain A1552

43.023

97.506

0.419

Multiple sequence alignment