Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K20_RS26830 Genome accession   NZ_CP027743
Coordinates   5946895..5948112 (+) Length   405 a.a.
NCBI ID   WP_124300535.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain CW2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5941895..5953112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K20_RS26815 (C4K20_5451) - 5942349..5945267 (+) 2919 WP_206428156.1 DUF748 domain-containing protein -
  C4K20_RS26820 (C4K20_5452) - 5945470..5945826 (-) 357 WP_124300534.1 BON domain-containing protein -
  C4K20_RS26825 (C4K20_5453) - 5946114..5946530 (-) 417 WP_009050907.1 pilin -
  C4K20_RS26830 (C4K20_5454) pilC 5946895..5948112 (+) 1218 WP_124300535.1 type II secretion system F family protein Machinery gene
  C4K20_RS26835 (C4K20_5455) pilD 5948115..5948987 (+) 873 WP_124300536.1 A24 family peptidase Machinery gene
  C4K20_RS26840 (C4K20_5456) coaE 5948984..5949607 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  C4K20_RS26845 (C4K20_5457) yacG 5949604..5949807 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K20_RS26850 (C4K20_5458) - 5949812..5950027 (-) 216 WP_009050911.1 hypothetical protein -
  C4K20_RS26855 (C4K20_5459) - 5950093..5950782 (-) 690 WP_038575688.1 energy-coupling factor ABC transporter permease -
  C4K20_RS26860 (C4K20_5460) - 5950779..5951246 (-) 468 WP_016703672.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44428.19 Da        Isoelectric Point: 10.4090

>NTDB_id=279896 C4K20_RS26830 WP_124300535.1 5946895..5948112(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain CW2]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSPFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRVSGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279896 C4K20_RS26830 WP_124300535.1 5946895..5948112(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain CW2]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCCTTTCCAACGCACACCTCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGACAGCTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCACTACGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGAAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTTGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGTGTGTTCGGCCTGCGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGGCCTGCTGAAACTGCCGTTGGTGGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTAAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGTGTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGATACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.679

100

0.657
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilG Neisseria meningitidis 44/76-A

41.294

99.259

0.41
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393

Multiple sequence alignment