Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C4J89_RS24670 Genome accession   NZ_CP027732
Coordinates   5383875..5385257 (-) Length   460 a.a.
NCBI ID   WP_124415778.1    Uniprot ID   -
Organism   Pseudomonas sp. R4-35-07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5378875..5390257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4J89_RS24655 (C4J89_4959) - 5380544..5382115 (-) 1572 WP_124415776.1 SPOR domain-containing protein -
  C4J89_RS24660 (C4J89_4960) aroB 5382126..5383226 (-) 1101 WP_124415777.1 3-dehydroquinate synthase -
  C4J89_RS24665 (C4J89_4961) aroK 5383352..5383870 (-) 519 WP_003171222.1 shikimate kinase AroK -
  C4J89_RS24670 (C4J89_4962) pilQ 5383875..5385257 (-) 1383 WP_124415778.1 type IV pilus secretin PilQ Machinery gene
  C4J89_RS24675 (C4J89_4963) - 5385270..5386241 (-) 972 WP_124415779.1 pilus assembly protein PilP -
  C4J89_RS24680 (C4J89_4964) - 5386238..5386789 (-) 552 WP_124415780.1 PilN domain-containing protein -
  C4J89_RS24685 (C4J89_4965) pilM 5386786..5387442 (-) 657 WP_164487608.1 type IV pilus assembly protein PilM -
  C4J89_RS24690 (C4J89_4967) - 5387663..5390125 (+) 2463 WP_372238991.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49924.25 Da        Isoelectric Point: 5.1395

>NTDB_id=279610 C4J89_RS24670 WP_124415778.1 5383875..5385257(-) (pilQ) [Pseudomonas sp. R4-35-07]
MKRIFSCFGVALWIAFTAPTAAAVPNRLDLIQLPPPESAASVGHTGDKLNLNFQNIDLRAALQQIADVAGLNLVAGDEVQ
GSITLRLKEVPWDQALDLVLQAKGLDKRVKAGVLLVAPADELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKL
FQSVTGFEGATDERGSVAVDDRTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKGLGARWGGRLNRG
NWSAGGIPKPQADGAQLPENPPSSPFVDLGSLTATSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARI
LKGTEIPYQESTSQGATSVSFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETI
VIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVRAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=279610 C4J89_RS24670 WP_124415778.1 5383875..5385257(-) (pilQ) [Pseudomonas sp. R4-35-07]
ATGAAAAGGATTTTCTCGTGCTTCGGTGTGGCGCTATGGATAGCGTTCACGGCGCCGACGGCTGCTGCTGTACCCAATCG
CCTCGACCTGATCCAATTGCCGCCGCCCGAAAGTGCTGCTTCGGTCGGCCACACGGGCGACAAGCTGAATCTCAATTTCC
AGAACATCGATTTGCGCGCAGCGCTGCAGCAAATCGCCGATGTCGCAGGCCTCAACCTGGTGGCCGGTGACGAGGTGCAA
GGTTCGATCACTCTGCGGCTCAAGGAGGTGCCTTGGGACCAGGCGCTGGACCTGGTGCTGCAAGCCAAGGGGTTGGACAA
ACGGGTGAAGGCCGGCGTATTGCTGGTGGCGCCGGCTGACGAATTGGCCGCTCGCGAACTGCTGACGTTGGAGGCGCGCA
AGCAGATGGCTGAGCTGGCACCGTTACGCCGGGAGTTGCTGCAGGTCAATTATGCCAAGGCAGCGGACCTGGCCAAGCTG
TTCCAGTCAGTGACCGGCTTTGAAGGCGCGACCGACGAGCGCGGCTCGGTGGCGGTCGACGACCGCACCAACAATATCAT
CGCCTACCAGACTGGAGAGCGGCTGGAAGAACTGCGGCGGATCGTGGCGCAGTTGGATGTGCCGGTGCGCCAGGTGATGA
TCGAGGCGCGCATCGTCGAGGCCAATGTCGATTACGACAAAGGCTTGGGCGCACGCTGGGGTGGGCGGCTCAATCGCGGC
AATTGGAGCGCCGGCGGTATCCCCAAGCCCCAGGCGGACGGCGCGCAGCTACCAGAGAACCCACCCAGCTCACCCTTCGT
CGACCTGGGTTCGCTGACCGCCACCTCGGGCCTGGGACTGGCCTTTATCACCGACAACCTGCTGCTGGACCTTGAGCTGA
CCGCCATGGAAAAGTCCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCACCTCGGACAAGGAAACCGCACGCATC
CTCAAGGGCACCGAGATCCCCTACCAGGAATCCACGTCCCAGGGCGCAACCTCGGTGTCGTTCAAGGAAGCCTCGCTGTC
ATTGGAGGTCACGCCGCAAATCACCCCCGACGATCATGTGATCATGGTGGTCAAAGTCACCAAGGACGAGCCCGACTATC
TGAACAAACTCAACGACGTTCCACCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAAGGATGGCGAGACCATT
GTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTTTTAGGCGATGTGCCGTATCTTGG
CCGCCTTTTCCGGCGCGATGTGCGGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAATAACC
AGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(295-451)

Secretin and TonB N terminus short domain (STN)

(72-118)

Bacterial type II/III secretion system short domain (Secretin_N)

(146-211)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.159

95.435

0.67
  comQ Acinetobacter baylyi ADP1

41.778

97.826

0.409
  pilQ Acinetobacter baumannii D1279779

40.389

100

0.407
  pilQ Acinetobacter baumannii strain A118

40.389

100

0.407
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.95

95.87

0.402
  pilQ Vibrio cholerae strain A1552

41.95

95.87

0.402
  pilQ Vibrio campbellii strain DS40M4

42.925

92.174

0.396
  pilQ Legionella pneumophila strain Lp02

43.026

91.957

0.396
  pilQ Legionella pneumophila strain ERS1305867

43.026

91.957

0.396

Multiple sequence alignment