Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K14_RS28370 Genome accession   NZ_CP027719
Coordinates   6224906..6226123 (+) Length   405 a.a.
NCBI ID   WP_124314374.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6219906..6231123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K14_RS28355 (C4K14_5743) - 6220360..6223278 (+) 2919 WP_206430348.1 DUF748 domain-containing protein -
  C4K14_RS28360 (C4K14_5744) - 6223481..6223837 (-) 357 WP_009050906.1 BON domain-containing protein -
  C4K14_RS28365 (C4K14_5745) - 6224125..6224541 (-) 417 WP_009050907.1 pilin -
  C4K14_RS28370 (C4K14_5746) pilC 6224906..6226123 (+) 1218 WP_124314374.1 type II secretion system F family protein Machinery gene
  C4K14_RS28375 (C4K14_5747) pilD 6226126..6226998 (+) 873 WP_009050909.1 A24 family peptidase Machinery gene
  C4K14_RS28380 (C4K14_5748) coaE 6226995..6227618 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  C4K14_RS28385 (C4K14_5749) yacG 6227615..6227818 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K14_RS28390 (C4K14_5750) - 6227824..6228039 (-) 216 WP_124314375.1 hypothetical protein -
  C4K14_RS28395 (C4K14_5751) - 6228105..6228794 (-) 690 WP_009050912.1 energy-coupling factor ABC transporter permease -
  C4K14_RS28400 (C4K14_5752) - 6228791..6229258 (-) 468 WP_016703672.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44448.24 Da        Isoelectric Point: 10.4090

>NTDB_id=279287 C4K14_RS28370 WP_124314374.1 6224906..6226123(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain P2]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLVRAQLRKQGISPARIRKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPMIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279287 C4K14_RS28370 WP_124314374.1 6224906..6226123(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain P2]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGGTCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGATCAGGAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGTGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGTGCGCTGCGCAAGAGGCCTCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAGACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACAGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGGCCTGCTGAAACTGCCATTGGTGGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAAGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATTTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGATACCCTGAGCAGTC
TCATGGAGCCCATGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.173

100

0.662
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.392

100

0.558
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio cholerae strain A1552

42.602

96.79

0.412
  pilC Vibrio campbellii strain DS40M4

39.801

99.259

0.395

Multiple sequence alignment