Detailed information NTG0279288

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C4K14_RS28375
• Genome accession: NZ_CP027719
• Coordinates: 6226126..6226998 (+)
• Length: 290 a.a.
• NCBI ID: WP_009050909.1
• Uniprot ID: A0AB33WWI2
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain P2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6221126..6231998

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4K14_RS28360 (C4K14_5744)	-	6223481..6223837 (-)	357	WP_009050906.1	BON domain-containing protein	-
C4K14_RS28365 (C4K14_5745)	-	6224125..6224541 (-)	417	WP_009050907.1	pilin	-
C4K14_RS28370 (C4K14_5746)	pilC	6224906..6226123 (+)	1218	WP_124314374.1	type II secretion system F family protein	Machinery gene
C4K14_RS28375 (C4K14_5747)	pilD	6226126..6226998 (+)	873	WP_009050909.1	A24 family peptidase	Machinery gene
C4K14_RS28380 (C4K14_5748)	coaE	6226995..6227618 (+)	624	WP_009045669.1	dephospho-CoA kinase	-
C4K14_RS28385 (C4K14_5749)	yacG	6227615..6227818 (+)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
C4K14_RS28390 (C4K14_5750)	-	6227824..6228039 (-)	216	WP_124314375.1	hypothetical protein	-
C4K14_RS28395 (C4K14_5751)	-	6228105..6228794 (-)	690	WP_009050912.1	energy-coupling factor ABC transporter permease	-
C4K14_RS28400 (C4K14_5752)	-	6228791..6229258 (-)	468	WP_016703672.1	hypothetical protein	-
C4K14_RS28405 (C4K14_5753)	-	6229714..6231150 (+)	1437	WP_009050914.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32050.22 Da
• Isoelectric Point: 7.9954

>NTDB_id=279288 C4K14_RS28375 WP_009050909.1 6226126..6226998(+) (pilD) [Pseudomonas chlororaphis subsp. aureofaciens strain P2]
MSLTEVLASYPLVFVASMLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHSIR
PWENIPVFSYLLLRGRCSACQAPIGRRYPLVELTCGLASAFVAWHFGFGWQAVLMLLLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGIALLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=279288 C4K14_RS28375 WP_009050909.1 6226126..6226998(+) (pilD) [Pseudomonas chlororaphis subsp. aureofaciens strain P2]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGATGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTTCTCAATGTGCTGATCTGGCGTCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTGGGGC
TGCCCGTCGAACCTGCCGGGCCGACCTACAACCTGCTGCTCCCGCATTCCCAGTGCCCTCACTGCGCACATTCGATCCGC
CCCTGGGAAAACATCCCGGTGTTCAGCTACCTGCTGCTGCGCGGACGCTGCTCCGCGTGCCAGGCACCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGTCC
TGATGCTGCTGCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAGCTGTTGCCCGACGTGCTGGTG
CTGCCGCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTTTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGTGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCCCTGGCGGGGATCGCCCTGCTGCGCCGCAACCGTGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.817	97.931	0.517
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	50.909	94.828	0.483
pilD	Acinetobacter nosocomialis M2	50.182	94.828	0.476
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448