Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C4K24_RS25935 Genome accession   NZ_CP027715
Coordinates   5746627..5747844 (+) Length   405 a.a.
NCBI ID   WP_038575694.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain M12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5741627..5752844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4K24_RS25920 (C4K24_5274) - 5742081..5744999 (+) 2919 WP_206428051.1 DUF748 domain-containing protein -
  C4K24_RS25925 (C4K24_5275) - 5745202..5745558 (-) 357 WP_009050906.1 BON domain-containing protein -
  C4K24_RS25930 (C4K24_5276) - 5745846..5746262 (-) 417 WP_009050907.1 pilin -
  C4K24_RS25935 (C4K24_5277) pilC 5746627..5747844 (+) 1218 WP_038575694.1 type II secretion system F family protein Machinery gene
  C4K24_RS25940 (C4K24_5278) pilD 5747847..5748719 (+) 873 WP_038575692.1 A24 family peptidase Machinery gene
  C4K24_RS25945 (C4K24_5279) coaE 5748716..5749339 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  C4K24_RS25950 (C4K24_5280) yacG 5749336..5749539 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  C4K24_RS25955 (C4K24_5281) - 5749544..5749759 (-) 216 WP_009050911.1 hypothetical protein -
  C4K24_RS25960 (C4K24_5282) - 5749825..5750514 (-) 690 WP_081359729.1 energy-coupling factor ABC transporter permease -
  C4K24_RS25965 (C4K24_5283) - 5750511..5750978 (-) 468 WP_016703672.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44400.14 Da        Isoelectric Point: 10.4090

>NTDB_id=279181 C4K24_RS25935 WP_038575694.1 5746627..5747844(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain M12]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSPFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=279181 C4K24_RS25935 WP_038575694.1 5746627..5747844(+) (pilC) [Pseudomonas chlororaphis subsp. aurantiaca strain M12]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGTATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCCGTTCCAGCGCACACCTCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGACAGCTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGCGCACTACGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGAAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTTGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGTGTGTTCGGCCTGCGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGGCCTGCTGAAACTGCCGTTGGTGGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTAAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCGTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGATACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.679

100

0.657
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

42.289

99.259

0.42
  pilG Neisseria meningitidis 44/76-A

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393

Multiple sequence alignment