Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6571_RS03980 Genome accession   NZ_CP027669
Coordinates   835254..836990 (+) Length   578 a.a.
NCBI ID   WP_106445543.1    Uniprot ID   A0A2S0MXE0
Organism   Simplicispira suum strain SC1-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830254..841990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6571_RS03950 (C6571_03950) proB 830775..831923 (-) 1149 WP_106445539.1 glutamate 5-kinase -
  C6571_RS03955 (C6571_03955) cgtA 831979..833061 (-) 1083 WP_106445540.1 Obg family GTPase CgtA -
  C6571_RS03960 (C6571_03960) rpmA 833163..833423 (-) 261 WP_106445541.1 50S ribosomal protein L27 -
  C6571_RS03965 (C6571_03965) rplU 833452..833763 (-) 312 WP_106445542.1 50S ribosomal protein L21 -
  C6571_RS03970 (C6571_03970) - 834061..834990 (+) 930 WP_106448027.1 polyprenyl synthetase family protein -
  C6571_RS03980 (C6571_03980) pilB 835254..836990 (+) 1737 WP_106445543.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6571_RS03985 (C6571_03985) pilC 837013..838233 (+) 1221 WP_106445544.1 type II secretion system F family protein Machinery gene
  C6571_RS03990 (C6571_03990) pilD 838233..839114 (+) 882 WP_106445545.1 prepilin peptidase Machinery gene
  C6571_RS03995 (C6571_03995) coaE 839132..839731 (+) 600 WP_106445546.1 dephospho-CoA kinase -
  C6571_RS04000 (C6571_04000) zapD 839811..840566 (+) 756 WP_106445547.1 cell division protein ZapD -
  C6571_RS04005 (C6571_04005) - 840580..840789 (+) 210 WP_106445548.1 DNA gyrase inhibitor YacG -
  C6571_RS04010 (C6571_04010) - 840830..841255 (-) 426 WP_106445549.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62811.19 Da        Isoelectric Point: 5.3457

>NTDB_id=278846 C6571_RS03980 WP_106445543.1 835254..836990(+) (pilB) [Simplicispira suum strain SC1-8]
MAAVDIAQKDASSIALPGLGRALISAGKLTQKAAEDIFRKSQASRTGFIAEVTGTGAVSASELAHTVSSIFGAPLLDLNA
IDPLRLPRDLLDAKICQAYRVLVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWVIAEYDKLARLIDSNTKSANEAI
DAYAGAGDFEFEDVAIEEAADTSDASGGDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRVDGELREIASPP
IAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIEALGYEPEEKE
RLLKAIERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARKLCPVCKEPIDIP
HEALVDAGYAEEEIDGSWVPYKPVGCSACNNGYKGRLGIYQVMPVTEEIQRIILRDGSALEIAAQAKLEGVRSLRESGLY
KAKLGVTSLEEVLAVTNL

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=278846 C6571_RS03980 WP_106445543.1 835254..836990(+) (pilB) [Simplicispira suum strain SC1-8]
ATGGCCGCTGTTGATATTGCCCAAAAAGACGCTTCGTCGATAGCCTTGCCAGGGTTGGGCCGGGCGTTGATTTCTGCGGG
AAAGCTGACGCAAAAGGCGGCCGAAGACATTTTCCGCAAATCGCAGGCCTCACGTACAGGCTTCATCGCCGAAGTCACCG
GCACTGGCGCCGTATCGGCCAGTGAATTGGCGCACACCGTTTCCTCCATCTTCGGCGCGCCGCTGCTTGACCTCAACGCC
ATTGACCCGCTTCGTCTACCGCGTGATCTGCTGGATGCAAAAATCTGTCAGGCTTACCGCGTTCTGGTTCTGAGCAAACG
CAACAACCGCCTCATCGTCGCAACTGCGGACCCAACAGATCAAGAAGCTGCAGAAAAAATCAAATTCACCACGCAGATGG
GGGTGGATTGGGTCATTGCCGAATACGACAAGCTCGCCCGCCTCATCGACTCCAACACCAAATCGGCCAACGAGGCGATA
GATGCTTACGCCGGTGCCGGCGATTTTGAATTTGAAGACGTTGCGATTGAAGAAGCAGCTGACACGTCGGACGCGTCGGG
CGGAGATGTCGAAGACGCACCCATCGTCAAATTCCTGCACAAGATGCTGCTCGATGCCTTCAACATGCGGGCATCAGACC
TGCATTTTGAACCCTATGAACACCATTACCGCGTGCGGTTTCGGGTGGACGGCGAGTTGCGCGAGATTGCCTCGCCACCG
ATTGCCATCAAAGACAAACTCGCCTCTCGAATCAAGGTAATTTCCCGACTTGATATTTCGGAGAAACGCGTTCCGCAGGA
CGGGCGCATGAAGCTGAAGGTGGGACCGGATCGGGTCATTGATTTTCGGGTCAGTACCTTGCCTACGCTTTTTGGGGAAA
AAATCGTCATCCGTATTCTGGACCCCAGCAGCGCGAAAATGGGAATTGAGGCGCTAGGCTACGAACCAGAAGAAAAAGAG
CGCCTGCTCAAAGCCATCGAGCGCCCTTACGGGATGATTCTGGTCACCGGGCCCACTGGATCGGGCAAGACGGTGTCCCT
GTATACCTGCCTCAATCTGTTGAACAAGCCGGGCGTGAACATCGCGACAGCCGAAGACCCCTCGGAAATCAACCTGCCTG
GCGTCAATCAGGTGAATGTGAATGAGAAGGCGGGGCTCACATTTGCTGTGGCCCTAAAGTCTTTTTTGCGCCAGGATCCC
GACATCATCATGGTGGGCGAAATCCGCGATTTGGAGACCGCCGACATCTCGATCAAGGCGGCACAAACCGGTCACTTGGT
GTTGTCGACACTGCATACCAATGATGCACCGACCACATTGACGCGCATGCGCAACATGGGGATTGCGCCCTTCAATATTG
CCTCCAGCGTGATACTGATTACGGCCCAACGCTTGGCGCGGAAATTGTGCCCCGTGTGCAAAGAGCCAATCGATATTCCG
CATGAAGCCTTGGTCGATGCAGGCTATGCAGAAGAAGAGATTGACGGCTCCTGGGTTCCTTACAAGCCCGTCGGTTGCTC
GGCCTGCAACAACGGCTACAAAGGCCGGCTTGGCATTTATCAAGTGATGCCAGTGACCGAAGAGATTCAGCGCATCATCC
TGCGCGATGGCAGTGCGCTTGAAATCGCCGCGCAAGCCAAGCTCGAAGGGGTGCGCTCATTGCGCGAATCGGGCCTTTAC
AAGGCCAAGCTCGGCGTGACTTCCCTTGAGGAAGTCCTTGCGGTTACGAACCTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-154)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0MXE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.345

98.27

0.524
  pilB Acinetobacter baumannii D1279779

52.083

99.654

0.519
  pilF Neisseria gonorrhoeae MS11

51.687

97.405

0.503
  pilB Legionella pneumophila strain ERS1305867

49.291

97.578

0.481
  pilB Vibrio campbellii strain DS40M4

46.032

98.097

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

97.578

0.45
  pilB Vibrio cholerae strain A1552

47.228

90.484

0.427
  pilF Thermus thermophilus HB27

38.908

98.27

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.525

92.215

0.374

Multiple sequence alignment