Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6570_RS17505 Genome accession   NZ_CP027666
Coordinates   3827226..3828965 (+) Length   579 a.a.
NCBI ID   WP_106704791.1    Uniprot ID   A0A2S0MK46
Organism   Ottowia oryzae strain KADR8-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3822226..3833965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6570_RS17485 (C6570_17475) - 3823444..3824148 (+) 705 WP_106704354.1 LuxR C-terminal-related transcriptional regulator -
  C6570_RS17490 (C6570_17480) - 3824499..3825344 (+) 846 WP_123812296.1 hypothetical protein -
  C6570_RS17495 (C6570_17485) - 3825495..3826235 (+) 741 WP_106704356.1 DUF6683 family protein -
  C6570_RS17500 (C6570_17490) - 3826322..3827032 (-) 711 WP_106704357.1 GNAT family N-acetyltransferase -
  C6570_RS17505 (C6570_17495) pilB 3827226..3828965 (+) 1740 WP_106704791.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6570_RS17510 (C6570_17500) pilC 3829001..3830224 (+) 1224 WP_106704358.1 type II secretion system F family protein Machinery gene
  C6570_RS17515 (C6570_17505) pilD 3830224..3831066 (+) 843 WP_106704359.1 A24 family peptidase Machinery gene
  C6570_RS17520 (C6570_17510) coaE 3831073..3831690 (+) 618 WP_106704360.1 dephospho-CoA kinase -
  C6570_RS17525 (C6570_17515) zapD 3831721..3832476 (+) 756 WP_106704361.1 cell division protein ZapD -
  C6570_RS17530 (C6570_17520) - 3832473..3832712 (+) 240 WP_106704362.1 DNA gyrase inhibitor YacG -
  C6570_RS17535 (C6570_17525) - 3832811..3833290 (-) 480 WP_106704363.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62954.36 Da        Isoelectric Point: 5.4663

>NTDB_id=278815 C6570_RS17505 WP_106704791.1 3827226..3828965(+) (pilB) [Ottowia oryzae strain KADR8-3]
MATAESVSRDAAPVALPGVGRALVLAGKLTQKVAEDTFKKARAGQKNFISELIGSGAIAASDLAHTLAQAFSAPLLDADA
LDPERLPKDLIDPKLSQTYRLLALSKRNGRLIVATADPSDHDAAERIKFSTQMGVEWVIAEFDKLSKLVEAHTKSAAESM
DSIVGGDFEFDEASLGAAVPAEEDDSAANDVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHQYRVRFRVDGELREIVSP
PVLIKEKLASRIKVLSRLDISEKRIPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEK
VRLLKAIERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKAFLRQD
PDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKAPMDI
PREALIEAGFDENELDGSWTPYKPVGCAACNNGYKGRVGIYEVMPISEDMQRIILADGSALEIGEQARREGVRSLRESAL
HKVKIGVTSLEEVLAVTNQ

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=278815 C6570_RS17505 WP_106704791.1 3827226..3828965(+) (pilB) [Ottowia oryzae strain KADR8-3]
ATGGCCACTGCCGAATCTGTTTCACGAGATGCCGCGCCCGTCGCGCTGCCTGGCGTGGGCCGCGCCCTGGTGCTGGCTGG
AAAACTGACCCAAAAAGTCGCCGAAGACACATTCAAGAAAGCACGCGCTGGCCAGAAAAACTTCATCTCCGAGCTGATCG
GCTCGGGCGCGATCGCCGCGTCGGATCTGGCCCATACCCTGGCCCAGGCTTTTTCGGCGCCGCTGCTGGATGCGGACGCA
CTCGATCCTGAGCGCCTGCCCAAGGACCTGATCGATCCAAAACTGTCGCAGACCTACCGCCTGCTGGCGCTCAGCAAACG
CAACGGCAGGCTGATCGTCGCCACGGCCGACCCGTCTGATCACGATGCGGCGGAACGCATCAAGTTCTCGACGCAGATGG
GCGTCGAATGGGTCATCGCCGAGTTCGACAAGCTGTCGAAGTTGGTGGAGGCCCACACCAAGTCCGCTGCCGAGTCGATG
GACAGCATCGTCGGCGGGGATTTTGAGTTCGACGAGGCGTCCTTGGGCGCTGCGGTTCCGGCAGAAGAGGACGATTCTGC
AGCGAACGATGTTGAAGACGCCCCGGTCGTCAAGTTTCTGCACAAGATGCTGATCGACGCCTTCAACATGCGCGCGTCGG
ACTTGCACTTCGAGCCGTATGAACACCAATACCGCGTGCGCTTTCGCGTGGACGGCGAATTGCGCGAGATCGTCTCTCCC
CCAGTGCTGATCAAAGAGAAGCTTGCCTCGCGCATCAAGGTGTTGTCGCGGCTGGATATTTCCGAGAAGCGGATTCCGCA
AGACGGCCGCATGAAGCTGAAAGTCGGCCCAGACCGCGTGATCGATTTTCGCGTGAGTACGCTGCCCACGCTTTTCGGTG
AAAAGATCGTTATTCGTATTCTCGACCCAAGCAGTGCCAAGCTCGGTATCGACGCGCTCGGCTACGAGCCCGAAGAGAAG
GTGCGCTTGCTCAAGGCGATTGAGCGCCCCTACGGCATGATCCTGGTGACCGGCCCCACCGGCTCCGGCAAGACCGTTTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCGGGCGTGAACATCGCGACTGCAGAAGACCCATCAGAGATCAACCTGC
CGGGCGTCAACCAGGTCAACGTCAACGAGAAGGCCGGTCTGACGTTTGCGGTCGCTCTCAAGGCTTTCCTGCGTCAGGAT
CCTGACATCATCATGGTCGGCGAGATCCGGGATCTCGAAACGGCCGACATCGCGATCAAGGCCGCGCAAACGGGGCACTT
GGTGCTGTCGACGCTGCACACGAACGACGCCCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACA
TCGCGTCCAGCGTGATCTTGATTACGGCACAGCGTCTGGCGCGCCGCCTTTGCCCTCAATGCAAGGCGCCGATGGACATC
CCCCGCGAGGCCTTGATCGAGGCTGGGTTTGATGAAAACGAGTTGGATGGAAGCTGGACGCCTTACAAACCGGTCGGCTG
CGCTGCCTGCAACAACGGCTATAAAGGACGCGTCGGCATTTATGAGGTGATGCCGATCAGTGAAGACATGCAGCGCATCA
TCCTCGCGGATGGCAGCGCATTGGAGATTGGCGAGCAGGCACGCAGGGAGGGCGTCCGCTCATTGCGCGAATCGGCGCTG
CACAAGGTCAAAATTGGCGTGACTTCGCTTGAAGAAGTTTTGGCCGTCACCAACCAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-157)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0MK46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.142

97.409

0.537
  pilB Acinetobacter baylyi ADP1

53.565

99.309

0.532
  pilF Neisseria gonorrhoeae MS11

52.837

97.409

0.515
  pilB Legionella pneumophila strain ERS1305867

50.707

97.755

0.496
  pilB Vibrio campbellii strain DS40M4

45.406

97.755

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.766

95.855

0.439
  pilB Vibrio cholerae strain A1552

47.399

89.637

0.425
  pilF Thermus thermophilus HB27

39.405

98.618

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.122

94.473

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

95.337

0.363

Multiple sequence alignment