Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PC2016_RS14565 Genome accession   NZ_CP027523
Coordinates   3186232..3187908 (+) Length   558 a.a.
NCBI ID   WP_131692482.1    Uniprot ID   -
Organism   Pseudoalteromonas carrageenovora strain KCTC 22325     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181232..3192908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PC2016_RS14545 (PC2016_2792) pilA/pilA1 3182549..3183001 (+) 453 WP_269459735.1 pilin Machinery gene
  PC2016_RS14550 (PC2016_2793) pilC 3183122..3184351 (+) 1230 WP_104643364.1 type II secretion system F family protein Machinery gene
  PC2016_RS14555 (PC2016_2794) pilD 3184569..3185462 (+) 894 WP_104643365.1 A24 family peptidase Machinery gene
  PC2016_RS14560 (PC2016_2795) coaE 3185464..3186105 (+) 642 WP_104643366.1 dephospho-CoA kinase -
  PC2016_RS14565 (PC2016_2796) pilB 3186232..3187908 (+) 1677 WP_131692482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PC2016_RS14570 (PC2016_2797) yacG 3187967..3188197 (+) 231 WP_058549398.1 DNA gyrase inhibitor YacG -
  PC2016_RS14575 (PC2016_2798) - 3188271..3188762 (-) 492 WP_104643368.1 Spy/CpxP family protein refolding chaperone -
  PC2016_RS14580 (PC2016_2799) - 3188938..3189630 (+) 693 WP_058549396.1 response regulator transcription factor -
  PC2016_RS14585 (PC2016_2800) - 3189608..3191023 (+) 1416 WP_104643369.1 ATP-binding protein -
  PC2016_RS14590 (PC2016_2801) - 3191027..3191974 (+) 948 WP_104643370.1 alpha/beta fold hydrolase -
  PC2016_RS14595 (PC2016_2802) - 3191947..3192816 (-) 870 WP_104643679.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62028.27 Da        Isoelectric Point: 5.2472

>NTDB_id=277683 PC2016_RS14565 WP_131692482.1 3186232..3187908(+) (pilB) [Pseudoalteromonas carrageenovora strain KCTC 22325]
MNINSPLLRKFITLGRIDADTVKAKQNEFASTAELISKCGKIDSLDLAEQCIDLFRVPYFDLKDFDPASIPADLVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDNKIEQLLDATGSLNLSDDEFKEFADLDTES
AKETAPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLSTKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEQLPSEELSRQGFTAKQIEDMTL
YAPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=277683 PC2016_RS14565 WP_131692482.1 3186232..3187908(+) (pilB) [Pseudoalteromonas carrageenovora strain KCTC 22325]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATTACCCTTGGCCGTATTGATGCCGACACGGTTAAAGCAAAACAAAA
CGAATTTGCCTCAACTGCTGAGCTTATTTCTAAATGCGGAAAAATCGATAGTCTAGATTTAGCTGAGCAATGTATTGATT
TGTTCCGCGTACCCTATTTTGATTTAAAAGACTTTGACCCTGCATCTATTCCAGCTGATCTTGTAAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTTGTTCAAAAAGATAGAAAAGTATACATTGCCGCCTCAGATCCTACTGATTACGGTGC
CTTTGAAAACTTTGAATTTAGTACGGGTTTATCGTGTGAAGTGGTGGTGGTTGATTACATTCAACTCGACAATAAAATAG
AGCAGTTACTAGATGCCACTGGTAGCTTAAATTTAAGTGATGATGAGTTTAAAGAGTTTGCCGATTTAGATACAGAAAGC
GCAAAAGAAACCGCTCCTAAAGATGATGACAAAGACGATGCGCCAATCATTGTTTACATCAATAAAATTTTAATGGATGC
CATTAAAAAAGGCGCGTCAGATTTACACTTTGAACCGTACGAGCATAAATACCGCATTCGCTTTCGTATAGACGGAATTT
TGCATGAAGTAGCCAGCCCACCTAATACGCTTTCTACTAAGCTTTCTGCACGTATAAAAGTAATGTCGAGGCTCGATATT
GCTGAAAAACGTAAACCGCAAGATGGCCGGATTAAATTAAAAATTACTGAACGAAAAAGTATCGATTTTCGTGTAAGTAC
TATGCCGACCCTTTGGGGCGAAAAAATAGTAATGCGTATCTTAGACTCATCAAGCGCTATGCTAGGCATTGATGTACTCG
GCTATGAAGAAGAGCAGAAAAAACTCTACATGGATGCCCTTGCTCAGCCACAAGGCATGATACTAGTTACAGGTCCTACA
GGTTCTGGTAAAACAGTATCACTTTATACAGGCTTAAATATTTTAAACCAGCCAGAGCGAAATATTAGTACCGCAGAGGA
TCCCGTAGAAATAAACCTCGAAGGGGTTAACCAAGTACAAATAAACCCTAAAGCCGAGATGACTTTTGCAAACGCACTGC
GTGCATTTTTACGTCAAGATCCTGATGTAGTAATGGTAGGTGAAATACGTGACCTTGAAACCGCTGAAATATCAATTAAG
GCAGCGCAAACGGGTCACTTAGTGTTATCAACACTGCATACAAACTCTGCTCCCGAAACCATTACACGTTTATTAAATAT
GGGCGTACCAGCTTATAACGTGGCAAGCTCTATTAGCTTAATTATAGCCCAGCGATTAGCGCGCCGATTATGTCCTAAGT
GTAAAACGCCAGAGCAACTACCGAGTGAAGAACTGTCTCGCCAAGGTTTTACAGCTAAGCAAATTGAAGATATGACACTC
TACGCACCTAAAGGCTGTGATAGTTGTACCGATGGCTATAAAGGCCGTGTAGGTATTTATGAAGTAATGCAAATTACGCC
AGAAATTGCACAAATTATTATGCGTGGTGGTAACTCACTAGAAATAGCAGAGGTTTCCCTTAAAGCTGGGTTTAATAATT
TACGCCTATCGGGTTTACGAAAAGCCGCCGATGGTTTAACGTCTCTGGCTGAAATAAACCGCGTAACTAATATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Acinetobacter baylyi ADP1

55.179

100

0.554
  pilB Vibrio cholerae strain A1552

53.876

92.473

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

52.354

95.161

0.498
  pilB Legionella pneumophila strain ERS1305867

54.102

91.756

0.496
  pilB Vibrio campbellii strain DS40M4

51.789

95.161

0.493
  pilF Neisseria gonorrhoeae MS11

49.617

93.548

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

37.138

100

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.132

97.491

0.362

Multiple sequence alignment