Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   PC2016_RS14545 Genome accession   NZ_CP027523
Coordinates   3182549..3183001 (+) Length   150 a.a.
NCBI ID   WP_269459735.1    Uniprot ID   A0A2K4XCP8
Organism   Pseudoalteromonas carrageenovora strain KCTC 22325     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3177549..3188001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PC2016_RS14525 (PC2016_2788) pdhR 3178383..3179132 (-) 750 WP_058549407.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PC2016_RS14530 (PC2016_2789) ampE 3179540..3180391 (-) 852 WP_104643361.1 beta-lactamase regulator AmpE -
  PC2016_RS14535 (PC2016_2790) ampD 3180405..3180959 (-) 555 WP_104643362.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PC2016_RS14540 (PC2016_2791) nadC 3181199..3182050 (+) 852 WP_104643363.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PC2016_RS14545 (PC2016_2792) pilA/pilA1 3182549..3183001 (+) 453 WP_269459735.1 pilin Machinery gene
  PC2016_RS14550 (PC2016_2793) pilC 3183122..3184351 (+) 1230 WP_104643364.1 type II secretion system F family protein Machinery gene
  PC2016_RS14555 (PC2016_2794) pilD 3184569..3185462 (+) 894 WP_104643365.1 A24 family peptidase Machinery gene
  PC2016_RS14560 (PC2016_2795) coaE 3185464..3186105 (+) 642 WP_104643366.1 dephospho-CoA kinase -
  PC2016_RS14565 (PC2016_2796) pilB 3186232..3187908 (+) 1677 WP_131692482.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15418.39 Da        Isoelectric Point: 4.0936

>NTDB_id=277680 PC2016_RS14545 WP_269459735.1 3182549..3183001(+) (pilA/pilA1) [Pseudoalteromonas carrageenovora strain KCTC 22325]
MKTMTQKQQGFTLIELMIVVAIIGILAAIALPAYQDYTARAQATEGLSATSGLRSDIGVYMSENGGATGIENDAAIGAQA
DALAGRYFAAEGVAVVADGVVNITFNDGAHSGETMALTPTVENSGQISRWVCSGLDAQYLPSACRAAAVE

Nucleotide


Download         Length: 453 bp        

>NTDB_id=277680 PC2016_RS14545 WP_269459735.1 3182549..3183001(+) (pilA/pilA1) [Pseudoalteromonas carrageenovora strain KCTC 22325]
ATGAAAACAATGACTCAAAAACAACAGGGTTTCACCCTAATCGAATTAATGATTGTAGTAGCAATCATCGGTATTCTTGC
TGCAATTGCATTGCCTGCATATCAAGATTACACTGCTCGCGCACAAGCAACAGAGGGTTTAAGTGCTACATCAGGGCTAC
GTTCAGATATTGGTGTTTATATGTCTGAAAACGGTGGTGCAACCGGCATAGAAAATGATGCTGCCATTGGTGCGCAAGCA
GATGCATTAGCTGGACGTTACTTTGCAGCTGAAGGTGTTGCTGTAGTTGCTGATGGTGTTGTTAACATTACTTTCAATGA
TGGCGCTCACTCAGGTGAGACTATGGCTTTGACACCTACAGTTGAAAATAGTGGTCAAATTTCACGTTGGGTTTGTAGTG
GTTTAGATGCTCAATACCTACCTTCTGCATGTAGAGCTGCTGCTGTTGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K4XCP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

52.903

100

0.547
  pilA Ralstonia pseudosolanacearum GMI1000

39.106

100

0.467
  pilA2 Legionella pneumophila strain ERS1305867

46.259

98

0.453
  pilA2 Legionella pneumophila str. Paris

47.222

96

0.453
  pilE Neisseria gonorrhoeae strain FA1090

37.349

100

0.413
  pilE Neisseria gonorrhoeae MS11

35.928

100

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.379

96.667

0.4
  comP Acinetobacter baylyi ADP1

39.597

99.333

0.393
  pilA Pseudomonas aeruginosa PAK

36.364

100

0.373
  pilA Acinetobacter baumannii strain A118

36.601

100

0.373

Multiple sequence alignment