Detailed information NTG0277682

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PC2016_RS14555
• Genome accession: NZ_CP027523
• Coordinates: 3184569..3185462 (+)
• Length: 297 a.a.
• NCBI ID: WP_104643365.1
• Uniprot ID: A0A2K4XCR3
• Organism: Pseudoalteromonas carrageenovora strain KCTC 22325
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3179569..3190462

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PC2016_RS14535 (PC2016_2790)	ampD	3180405..3180959 (-)	555	WP_104643362.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
PC2016_RS14540 (PC2016_2791)	nadC	3181199..3182050 (+)	852	WP_104643363.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PC2016_RS14545 (PC2016_2792)	pilA/pilA1	3182549..3183001 (+)	453	WP_269459735.1	pilin	Machinery gene
PC2016_RS14550 (PC2016_2793)	pilC	3183122..3184351 (+)	1230	WP_104643364.1	type II secretion system F family protein	Machinery gene
PC2016_RS14555 (PC2016_2794)	pilD	3184569..3185462 (+)	894	WP_104643365.1	A24 family peptidase	Machinery gene
PC2016_RS14560 (PC2016_2795)	coaE	3185464..3186105 (+)	642	WP_104643366.1	dephospho-CoA kinase	-
PC2016_RS14565 (PC2016_2796)	pilB	3186232..3187908 (+)	1677	WP_131692482.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PC2016_RS14570 (PC2016_2797)	yacG	3187967..3188197 (+)	231	WP_058549398.1	DNA gyrase inhibitor YacG	-
PC2016_RS14575 (PC2016_2798)	-	3188271..3188762 (-)	492	WP_104643368.1	Spy/CpxP family protein refolding chaperone	-
PC2016_RS14580 (PC2016_2799)	-	3188938..3189630 (+)	693	WP_058549396.1	response regulator transcription factor	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 32847.16 Da
• Isoelectric Point: 7.9139

>NTDB_id=277682 PC2016_RS14555 WP_104643365.1 3184569..3185462(+) (pilD) [Pseudoalteromonas carrageenovora strain KCTC 22325]
MQSIIEVMQSQLWFYLTTVGLVSLCVGSFLNVVIYRLPLMMQREWQSECRLLLEDELTANKSKQANTSEPFNLVKPNSTC
PKCKTAIKPWQNIPVISWLLLKAKCASCSNPISVRYPAVEAITAILSLIVAYSFGATEQALLYIFVTWILVALTFIDIDH
MLLPDQLTLPLVWLALIAAVAGITITPRDAIMGAAFGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAVFGALLGWQSLLTI
ILLSSVVGAIIGIALLSIQGKDKATPIPFGPYLAIAGWITLLWGSQIQNMYFNLIGY

Nucleotide

• Length: 894 bp

>NTDB_id=277682 PC2016_RS14555 WP_104643365.1 3184569..3185462(+) (pilD) [Pseudoalteromonas carrageenovora strain KCTC 22325]
ATGCAAAGTATTATTGAAGTAATGCAAAGCCAATTGTGGTTTTATTTAACCACCGTTGGCTTAGTCAGTTTATGTGTAGG
CAGTTTTTTAAATGTGGTTATTTACCGCTTACCACTCATGATGCAACGCGAATGGCAAAGCGAATGTAGGCTACTACTAG
AAGATGAACTTACTGCCAATAAATCTAAGCAAGCTAACACCAGTGAGCCTTTTAACTTAGTAAAACCTAACTCTACCTGC
CCAAAATGTAAAACAGCCATTAAACCTTGGCAAAATATTCCTGTTATTAGCTGGCTTTTGCTAAAAGCTAAATGCGCGAG
TTGTAGTAACCCTATTTCAGTTCGTTACCCTGCTGTTGAGGCAATTACAGCCATACTAAGCCTTATTGTTGCTTATAGCT
TTGGTGCTACAGAGCAAGCCCTTTTATATATTTTTGTAACATGGATTTTAGTAGCGCTAACCTTTATCGATATTGACCAC
ATGCTGCTACCCGATCAGCTAACTTTACCGCTTGTTTGGCTGGCACTTATTGCCGCTGTTGCCGGAATTACAATTACGCC
AAGAGATGCAATTATGGGTGCCGCTTTTGGCTATTTAAGCTTATGGAGTGTGTTTTGGTTATTTAAGTTACTAACCGGCA
AAGAAGGCATGGGTTATGGCGATTTTAAACTACTCGCTGTATTTGGCGCACTTCTTGGTTGGCAATCACTTTTAACTATT
ATTCTGCTTTCGAGCGTAGTGGGTGCAATTATAGGTATTGCCCTTTTAAGCATTCAAGGTAAAGACAAAGCCACACCAAT
CCCTTTTGGCCCTTATTTAGCAATAGCGGGTTGGATAACACTATTGTGGGGCAGTCAAATTCAAAATATGTATTTTAATT
TAATTGGCTATTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-134)

• Type IV leader peptidase family (Peptidase_A24) (145-253)

3D structure

• AlphaFold DB: A0A2K4XCR3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	53.082	98.316	0.522
pilD	Acinetobacter baumannii D1279779	51.536	98.653	0.508
pilD	Acinetobacter nosocomialis M2	51.195	98.653	0.505
pilD	Vibrio cholerae strain A1552	50.859	97.98	0.498
pilD	Neisseria gonorrhoeae MS11	45.489	89.562	0.407