Detailed information NTG0027470

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLMG_RS09780
• Genome accession: NC_009004
• Coordinates: 1937133..1937789 (-)
• Length: 218 a.a.
• NCBI ID: WP_014735120.1
• Uniprot ID: -
• Organism: Lactococcus cremoris subsp. cremoris MG1363
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1932133..1942789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLMG_RS09755 (llmg_1950)	atpF	1932204..1932710 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLMG_RS09760 (llmg_1951)	atpB	1932725..1933438 (-)	714	WP_011835693.1	F0F1 ATP synthase subunit A	-
LLMG_RS09765 (llmg_1952)	-	1933485..1933700 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLMG_RS09770 (llmg_1953)	-	1933883..1934659 (-)	777	WP_011835694.1	alpha/beta hydrolase	-
LLMG_RS09775 (llmg_pseudo_60)	-	1934943..1937152 (-)	2210	Protein_1905	DNA internalization-related competence protein ComEC/Rec2	-
LLMG_RS09780 (llmg_1955)	comEA	1937133..1937789 (-)	657	WP_014735120.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLMG_RS09785 (llmg_1956)	-	1937847..1939208 (-)	1362	WP_011835696.1	ABC transporter permease	-
LLMG_RS09790 (llmg_1957)	-	1939205..1940137 (-)	933	WP_011835697.1	ABC transporter ATP-binding protein	-
LLMG_RS09800 (llmg_1959)	-	1940757..1941317 (-)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
LLMG_RS09805 (llmg_1960)	-	1941491..1942669 (-)	1179	WP_011835700.1	SLC13 family permease	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23674.86 Da
• Isoelectric Point: 5.1730

>NTDB_id=27470 LLMG_RS09780 WP_014735120.1 1937133..1937789(-) (comEA) [Lactococcus cremoris subsp. cremoris MG1363]
MEKILDKLKEYWKIMLLVVCALIAGGILYIFTNSPKPAETLSIENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADPKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQVSTTSGPKVNIN
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=27470 LLMG_RS09780 WP_014735120.1 1937133..1937789(-) (comEA) [Lactococcus cremoris subsp. cremoris MG1363]
ATGGAAAAAATTTTAGATAAATTAAAAGAATACTGGAAAATAATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAAT
TCTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAATAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCCAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAGTAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.165	100	0.982
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.41	100	0.431
comEA/celA/cilE	Streptococcus mitis SK321	41.778	100	0.431
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.529	100	0.422
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	37.021	100	0.399
comEA	Staphylococcus aureus MW2	35.931	100	0.381
comEA	Staphylococcus aureus N315	36.866	99.541	0.367