Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC77_RS02925 Genome accession   NZ_CP027030
Coordinates   593168..594856 (-) Length   562 a.a.
NCBI ID   WP_080538712.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_077     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588168..599856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC77_RS02895 (FORC77_0514) rplS 588705..589058 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  FORC77_RS02900 (FORC77_0515) yacG 589291..589485 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  FORC77_RS02905 (FORC77_0516) zapD 589548..590288 (-) 741 WP_011079546.1 cell division protein ZapD -
  FORC77_RS02910 (FORC77_0517) coaE 590321..590929 (-) 609 WP_039554617.1 dephospho-CoA kinase -
  FORC77_RS02915 (FORC77_0518) pilD 590931..591800 (-) 870 WP_131069647.1 A24 family peptidase Machinery gene
  FORC77_RS02920 (FORC77_0519) pilC 591894..593120 (-) 1227 WP_011079549.1 type II secretion system F family protein Machinery gene
  FORC77_RS02925 (FORC77_0520) pilB 593168..594856 (-) 1689 WP_080538712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC77_RS02930 (FORC77_0521) pilA 594861..595286 (-) 426 WP_072608924.1 pilin Machinery gene
  FORC77_RS02935 (FORC77_0522) nadC 595509..596396 (-) 888 WP_072608923.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC77_RS02940 (FORC77_0523) ampD 596489..597061 (+) 573 WP_072608922.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC77_RS02945 (FORC77_0524) pdhR 597449..598216 (+) 768 WP_131069648.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62431.41 Da        Isoelectric Point: 5.5349

>NTDB_id=274591 FORC77_RS02925 WP_080538712.1 593168..594856(-) (pilB) [Vibrio vulnificus strain FORC_077]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=274591 FORC77_RS02925 WP_080538712.1 593168..594856(-) (pilB) [Vibrio vulnificus strain FORC_077]
GTGCAATCCAACCTTGCCACGATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGTACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAG
CTGATAACCCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCCGGTTTAATACTCGCAGTTGCAGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCTGATTGCCAAGAGCTGCAAGCGG
CGATTCGCAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTGAGCCGCTTTAT
CAACCAAATCCTGCTTGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCAGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio parahaemolyticus RIMD 2210633

75.979

100

0.76
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.787

99.466

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment